| General information | | DisProt: | DP00003 | | Name: | Early E2A DNA-binding protein | | Synonym(s): | DNB2_ADE05
Adenovirus ssDNA binding protein
| | First appeared in release: | Release 2.0 (02/14/2005) | | UniProt: | P03265 | | UniGene: | | | SwissProt: | DNB2_ADE05 | | TrEMBL: | | | NCBI (GI): | 118736 | | Source organism: | Human adenovirus C serotype 5 (HAdV-5)(Human adenovirus 5) | | Sequence length: | 529 | | Percent disordered: | 10% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MASREEEQRE TTPERGRGAA RRPPTMEDVS SPSPSPPPPR APPKKRMRRR IESEDEEDSS - 60
QDALVPRTPS PRPSTSAADL AIAPKKKKKR PSPKPERPPS PEVIVDSEEE REDVALQMVG - 120
FSNPPVLIKH GKGGKRTVRR LNEDDPVARG MRTQEEEEEP SEAESEITVM NPLSVPIVSA - 180
WEKGMEAARA LMDKYHVDND LKANFKLLPD QVEALAAVCK TWLNEEHRGL QLTFTSNKTF - 240
VTMMGRFLQA YLQSFAEVTY KHHEPTGCAL WLHRCAEIEG ELKCLHGSIM INKEHVIEMD - 300
VTSENGQRAL KEQSSKAKIV KNRWGRNVVQ ISNTDARCCV HDAACPANQF SGKSCGMFFS - 360
EGAKAQVAFK QIKAFMQALY PNAQTGHGHL LMPLRCECNS KPGHAPFLGR QLPKLTPFAL - 420
SNAEDLDADL ISDKSVLASV HHPALIVFQC CNPVYRNSRA QGGGPNCDFK ISAPDLLNAL - 480
VMVRSLWSEN FTELPRMVVP EFKWSTKHQY RNVSLPVAHS DARQNPFDF
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| Functional narrative |
The human adenovirus type 5 is a virus that attacks the respiratory system. Often if causes infections in the upper respiratory system, and is accompanied by fever and a runny nose. Occasionally it may cause pneumonia. It accumulates in the nucleus of infected cells. The adenovirus binding protein is essential in the unwinding and replication of DNA. It also helps in viron assembly, host range determination, control of transcription, and stability of RNA. It is also stabilizes the DNA binding complex after binding to DNA has taken place.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | Flexible Loop | | Location: | 297 - 331 | | Length: | 35 | | Region sequence: |
IEMDVTSENGQRALKEQSSKAKIVKNRWGRNVVQI | | Modification type: | Native
| | PDB: | 1ADT:A, 1ADV:A, 1ADV:B, 1ANV:A | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular recognition effectors
| | Functional subclasses: | DNA unwinding
Protein-DNA binding
| Detection methods:
- X-ray crystallography (pH: 7.2; ethanol 5 %; NaCl 1.6 M; phosphate (buffer) 100 mM; protein 0.5 %)
| References:
- Dekker J, Kanellopoulos PN, van Oosterhout JA, Stier G, Tucker PA, van der Vliet PC. "ATP-independent DNA unwinding by the adenovirus single-stranded DNA binding protein requires a flexible DNA binding loop." J Mol Biol. 1998; 277(4): 825-38. PubMed: 9545375
- Tsernoglou D, Tsugita A, Tucker AD, van der Vliet PC. "Characterization of the chymotryptic core of the adenovirus DNA-binding protein." FEBS Lett. 1985; 188(2): 248-52. PubMed: 4040872
- Tucker, P., Tsernoglou, D., Tucker, A., Coenjaerts, F., Leenders, H., Vliet, P. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding." 1994; 13(13): 2994-3002. PubMed: 8039495
| Comments:This region is known as the flexible loop. It goes from disordered to ordered upon binding to DNA, helps to stabilize the binding complex and plays an important role in DNA replication.
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| Region 2 | | Type: | Disordered - Extended | | Name: | | | Location: | 401 - 406 | | Length: | 6 | | Region sequence: |
KPGHAP | | Modification type: | Native
| | PDB: | 1ADT:A, 1ADV:A, 1ADV:B, 1ANV:A | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (pH: 7.2; ethanol 5 %; NaCl 1.6 M; phosphate (buffer) 100 mM; protein 0.5 %)
| References:
- Dekker J, Kanellopoulos PN, van Oosterhout JA, Stier G, Tucker PA, van der Vliet PC. "ATP-independent DNA unwinding by the adenovirus single-stranded DNA binding protein requires a flexible DNA binding loop." J Mol Biol. 1998; 277(4): 825-38. PubMed: 9545375
- Tsernoglou D, Tsugita A, Tucker AD, van der Vliet PC. "Characterization of the chymotryptic core of the adenovirus DNA-binding protein." FEBS Lett. 1985; 188(2): 248-52. PubMed: 4040872
- Tucker, P., Tsernoglou, D., Tucker, A., Coenjaerts, F., Leenders, H., Vliet, P. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding." 1994; 13(13): 2994-3002. PubMed: 8039495
| Comments:This region is part of the C-terminal portion of the protein which is important in chain formation and DNA replication.
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| Region 3 | | Type: | Disordered - Extended | | Name: | | | Location: | 453 - 464 | | Length: | 12 | | Region sequence: |
PVYRNSRAQGGG | | Modification type: | Native
| | PDB: | 1ADT:A, 1ADV:A, 1ADV:B, 1ANV:A | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (pH: 7.2; ethanol 5 %; NaCl 1.6 M; phosphate (buffer) 100 mM; protein 0.5 %)
| References:
- Dekker J, Kanellopoulos PN, van Oosterhout JA, Stier G, Tucker PA, van der Vliet PC. "ATP-independent DNA unwinding by the adenovirus single-stranded DNA binding protein requires a flexible DNA binding loop." J Mol Biol. 1998; 277(4): 825-38. PubMed: 9545375
- Tsernoglou D, Tsugita A, Tucker AD, van der Vliet PC. "Characterization of the chymotryptic core of the adenovirus DNA-binding protein." FEBS Lett. 1985; 188(2): 248-52. PubMed: 4040872
- Tucker, P., Tsernoglou, D., Tucker, A., Coenjaerts, F., Leenders, H., Vliet, P. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding." 1994; 13(13): 2994-3002. PubMed: 8039495
| Comments:This region is part of the C-terminal portion of the protein which is important for chain formation and DNA replication.
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| Region 4 | | Type: | Ordered | | Name: | | | Location: | 174 - 296 | | Length: | 123 | | Region sequence: |
SVPIVSAWEKGMEAARALMDKYHVDNDLKANFKLLPDQVEALAAVCKTWLNEEHRGLQLT
FTSNKTFVTMMGRFLQAYLQSFAEVTYKHHEPTGCALWLHRCAEIEGELKCLHGSIMINK
EHV | | Modification type: | | | PDB: | 1ADT:A, 1ADV:A, 1ADV:B, 1ANV:A | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
| References:
- Dekker J, Kanellopoulos PN, van Oosterhout JA, Stier G, Tucker PA, van der Vliet PC. "ATP-independent DNA unwinding by the adenovirus single-stranded DNA binding protein requires a flexible DNA binding loop." J Mol Biol. 1998; 277(4): 825-38. PubMed: 9545375
- Tsernoglou D, Tsugita A, Tucker AD, van der Vliet PC. "Characterization of the chymotryptic core of the adenovirus DNA-binding protein." FEBS Lett. 1985; 188(2): 248-52. PubMed: 4040872
- Tucker, P., Tsernoglou, D., Tucker, A., Coenjaerts, F., Leenders, H., Vliet, P. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding." 1994; 13(13): 2994-3002. PubMed: 8039495
| Comments:
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| Region 5 | | Type: | Ordered | | Name: | | | Location: | 332 - 400 | | Length: | 69 | | Region sequence: |
SNTDARCCVHDAACPANQFSGKSCGMFFSEGAKAQVAFKQIKAFMQALYPNAQTGHGHLL
MPLRCECNS | | Modification type: | | | PDB: | 1ADT:A, 1ADV:A, 1ADV:B, 1ANV:A | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
| References:
- Dekker J, Kanellopoulos PN, van Oosterhout JA, Stier G, Tucker PA, van der Vliet PC. "ATP-independent DNA unwinding by the adenovirus single-stranded DNA binding protein requires a flexible DNA binding loop." J Mol Biol. 1998; 277(4): 825-38. PubMed: 9545375
- Tsernoglou D, Tsugita A, Tucker AD, van der Vliet PC. "Characterization of the chymotryptic core of the adenovirus DNA-binding protein." FEBS Lett. 1985; 188(2): 248-52. PubMed: 4040872
- Tucker, P., Tsernoglou, D., Tucker, A., Coenjaerts, F., Leenders, H., Vliet, P. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding." 1994; 13(13): 2994-3002. PubMed: 8039495
| Comments:
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| Region 6 | | Type: | Ordered | | Name: | | | Location: | 407 - 452 | | Length: | 46 | | Region sequence: |
FLGRQLPKLTPFALSNAEDLDADLISDKSVLASVHHPALIVFQCCN | | Modification type: | | | PDB: | 1ADT:A, 1ADV:A, 1ADV:B, 1ANV:A | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
| References:
- Dekker J, Kanellopoulos PN, van Oosterhout JA, Stier G, Tucker PA, van der Vliet PC. "ATP-independent DNA unwinding by the adenovirus single-stranded DNA binding protein requires a flexible DNA binding loop." J Mol Biol. 1998; 277(4): 825-38. PubMed: 9545375
- Tsernoglou D, Tsugita A, Tucker AD, van der Vliet PC. "Characterization of the chymotryptic core of the adenovirus DNA-binding protein." FEBS Lett. 1985; 188(2): 248-52. PubMed: 4040872
- Tucker, P., Tsernoglou, D., Tucker, A., Coenjaerts, F., Leenders, H., Vliet, P. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding." 1994; 13(13): 2994-3002. PubMed: 8039495
| Comments:
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| Region 7 | | Type: | Ordered | | Name: | | | Location: | 465 - 529 | | Length: | 65 | | Region sequence: |
PNCDFKISAPDLLNALVMVRSLWSENFTELPRMVVPEFKWSTKHQYRNVSLPVAHSDARQ
NPFDF | | Modification type: | | | PDB: | 1ADT:A, 1ADV:A, 1ADV:B, 1ANV:A | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
| References:
- Dekker J, Kanellopoulos PN, van Oosterhout JA, Stier G, Tucker PA, van der Vliet PC. "ATP-independent DNA unwinding by the adenovirus single-stranded DNA binding protein requires a flexible DNA binding loop." J Mol Biol. 1998; 277(4): 825-38. PubMed: 9545375
- Tsernoglou D, Tsugita A, Tucker AD, van der Vliet PC. "Characterization of the chymotryptic core of the adenovirus DNA-binding protein." FEBS Lett. 1985; 188(2): 248-52. PubMed: 4040872
- Tucker, P., Tsernoglou, D., Tucker, A., Coenjaerts, F., Leenders, H., Vliet, P. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding." 1994; 13(13): 2994-3002. PubMed: 8039495
| Comments:
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