| General information | | DisProt: | DP00008 | | Name: | POU domain class 2-associating factor 1 | | Synonym(s): | OBF1_MOUSE
B-cell-specific coactivator OBF-1
OCT-binding factor 1
B-cell-specific coactivator OCA-B
OCA-B
B-cell-specific coactivator BOB-1
BOB-1
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | Q64693 | | UniGene: | Mm.897 | | SwissProt: | OBF1_MOUSE | | TrEMBL: | | | NCBI (GI): | 2842674 | | Source organism: | Mus musculus (Mouse) | | Sequence length: | 256 | | Percent disordered: | 100% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAG VLPHQPLATY - 60
STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLHALA PWTPYTEYVS HEAVSCPYST - 120
DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTPRSTATPA VGPQLEGPEH QAPLTYFPWP - 180
QPLSTLPTSS LQYQPPAPTL SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE - 240
ESNTYELNHT LSVEGF
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| Functional narrative |
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. Its boost the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centres.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 256 | | Length: | 256 | | Region sequence: |
MLWQKSTAPEQAPAPPRPYQGVRVKEPVKELLRRKRGHTSVGAAGPPTAGVLPHQPLATY
STVGPSCLDMEVSASTVTEEGTLCAGWLSQPAPATLHALAPWTPYTEYVSHEAVSCPYST
DMYVQPVCPSYTVVGPSSVLTYASPPLITNVTPRSTATPAVGPQLEGPEHQAPLTYFPWP
QPLSTLPTSSLQYQPPAPTLSGPQFVQLPISIPEPVLQDMDDPRRAISSLTIDKLLLEEE
ESNTYELNHTLSVEGF | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | | | Functional subclasses: | Protein-DNA binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (DTT 5 mM; NaCl 50 mM; path length (quartz cuvette) 0.01 mm; Station 3.1 Daresbury Synchrotron (radiation source))
- Small-angle X-ray scattering (SAXS)
- Nuclear magnetic resonance (NMR) (295 K; )
| References:
- Chang JF, Phillips K, Lundback T, Gstaiger M, Ladbury JE, Luisi B. "Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding." J Mol Biol. 1999; 288(5): 941-52. PubMed: 10329190
- Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B. "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator." Biochemistry. 2001; 40(22): 6580-8. PubMed: 11380252
| Comments:
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| References |
- Chang JF, Phillips K, Lundback T, Gstaiger M, Ladbury JE, Luisi B. "Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding." J Mol Biol. 1999; 288(5): 941-52. PubMed: 10329190
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| Comments |
AV (6-15-2010) PubMed: 11380252
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