| General information | | DisProt: | DP00024 | | Name: | Protein E7 | | Synonym(s): | VE7_HPV16
| | First appeared in release: | Release 2.2 (04/14/2005) | | UniProt: | P03129 | | UniGene: | | | SwissProt: | VE7_HPV16 | | TrEMBL: | | | NCBI (GI): | 137791 | | Source organism: | Human papillomavirus type 16 | | Sequence length: | 98 | | Percent disordered: | 100% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MHGDTPTLHE YMLDLQPETT DLYCYEQLSD SSEEEDEIDG PAGQAEPDRA HYNIVTFCCK - 60
CDSTLRLCVQ STHVDIRTLE DLLMGTLGIV CPICSQKP
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| Functional narrative |
E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription By similarity.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 98 | | Length: | 98 | | Region sequence: |
MHGDTPTLHEYMLDLQPETTDLYCYEQLSDSSEEEDEIDGPAGQAEPDRAHYNIVTFCCK
CDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | | | Functional subclasses: | Metal binding
Phosphorylation
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (278 K; pH: 7.5; DTT 0.1 mM; EDTA 0.1 mM; Jasco J-600 (spectropolarimeter); path length (quartz cell) 0.2 cm; protein (sample) 0.78 mM; Tris 1 mM; zinc or cadmium chloride 1 mM)
| References:
- Pahel G, Aulabaugh A, Short SA, Barnes JA, Painter GR, Ray P, Phelps WC. "Structural and functional characterization of the HPV16 E7 protein expressed in bacteria." J Biol Chem. 1993; 268(34): 26018-25. PubMed: 8245034
| Comments:
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| References |
- Chemes LB, Sanchez IE, Smal C, de Prat-Gay G. "Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein." FEBS J. . PubMed: 20088881
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| Comments |
AV (6-17-10) PubMed: 20088881
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| If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us. |
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