DP00032: Glycyl-tRNA synthetaseFASTA viewXML view

General information
DisProt:DP00032
Name:Glycyl-tRNA synthetase
Synonym(s):SYG_THET8
EC=6.1.1.14
Glycine--tRNA ligase
GlyRS
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P56206
UniGene: 
SwissProt: SYG_THET8
TrEMBL:  
NCBI (GI): 62296700
Source organism:Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Sequence length:505
Percent disordered:13%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
PASSLDELVA LCKRRGFIFQ SSEIYGGLQG VYDYGPLGVE LKNNLKQAWW RRNVYERDDM - 60
EGLDASVLTH RLVLHYSGHE ATFADPMVDN RITKKRYRLD HLLKEQPEEV LKRLYRAMEV - 120
EEENLHALVQ AMMQAPERAG GAMTAAGVLD PASGEPGDWT PPRYFNMMFK TYVGPVEDEA - 180
SLAYLRPETA QGIFVNFKNV LDATSRKLPF GIAQIGKAFR NEITPRNFIF RVREFEQMEI - 240
EYFVRPGEDE YWHRYWVEER LKWWQEMGLS RENLVPYQQP PEELAHYAKA TVDILYRFPH - 300
GLEELEGIAN RTDFDLGSHT KDQEALGITA RVLRNEHSTQ RLAYRDPETG KWFVPYVIEP - 360
SAGVDRGVLA LLAEAFTREE LPNGEERIVL KLKPQLAPIK VAVIPLVKNR PEITEYAKRL - 420
KARLLALGLG RVLYEDTGNI GKAYRRHDEV GTPFAVTVDY DTIGQSKDGT TRLKDTVTVR - 480
DRDTMEQIRL HVDELEGFLR ERLRW

Region 1: 91-158

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:insertion domain
Location:91 - 158
Length:68
Region sequence:

RITKKRYRLDHLLKEQPEEVLKRLYRAMEVEEENLHALVQAMMQAPERAGGAMTAAGVLD
PASGEPGD

Modification type:  
PDB: 1ATI:A, 1ATI:B, 1B76:A, 1B76:B
Structural/functional type: Relationship to function unknown
Functional classes: Molecular assembly
Functional subclasses: Fatty acylation (myristolation and palmitoylation)
Protein-tRNA binding
Detection methods:
  1. X-ray crystallography (273 K; ammonium sulfate 0.4 mM; ATP 5 mM; MgCl2 2 mM)
References:
  1. Arnez JG, Dock-Bregeon AC, Moras D. "Glycyl-tRNA synthetase uses a negatively charged pit for specific recognition and activation of glycine." J Mol Biol. 1999; 286(5): 1449-59. PubMed: 10064708

  2. Logan DT, Mazauric MH, Kern D, Moras D. "Crystal structure of glycyl-tRNA synthetase from Thermus thermophilus." Embo J. 1995; 14(17): 4156-67. PubMed: 7556056
Comments:
The PDB chains 1B76 A and B have the disordered region spanning residues 96-158.


References

  1. Mazauric MH, Keith G, Logan D, Kreutzer R, Giege R, Kern D. "Glycyl-tRNA synthetase from Thermus thermophilus--wide structural divergence with other prokaryotic glycyl-tRNA synthetases and functional inter-relation with prokaryotic and eukaryotic glycylation systems." Eur J Biochem. 1998; 251(3): 744-57. PubMed: 9490048



Comments


Av 6-28-2010 PubMed: 9490048


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