| General information | | DisProt: | DP00081 | | Name: | Transcription initiation factor TFIID subunit 1 | | Synonym(s): | TAF1_DROME
EC=2.7.11.1
Transcription initiation factor TFIID 230 kDa subunit
TAFII-230
TAFII250
TBP-associated factor 230 kDa
p230
Transcription factor dTAFII230
| | First appeared in release: | Release 1.1 (12/01/2003) | | UniProt: | P51123 | | UniGene: | Dm.7380 | | SwissProt: | TAF1_DROME | | TrEMBL: | | | NCBI (GI): | 71153181 | | Source organism: | Drosophila melanogaster (Fruit fly) | | Sequence length: | 2068 | | Percent disordered: | 3% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MEMESDNSDD EGSIGNGLDL TGILFGNIDS EGRLLQDDDG EGRGGTGFDA ELRENIGSLS - 60
KLGLDSMLLE VIDLKEAEPP SDDEEEEDAR PSAVSASGGM SAFDALKAGV KREEREDGAV - 120
KAQDDAIDYS DITELSEDCP RTPPEETSTY DDLEDAIPAS KVEAKLTKDD KELMPPPSAP - 180
MRSGSGGGIE EPAKSNDASS PSDDSKSTDS KDADRKLDTP LADILPSKYQ NVDVRELFPD - 240
FRPQKVLRFS RLFGPGKPTS LPQIWRHVRQ SRRKRNQSRD QKTTNTGGSD SPSDTEEPRK - 300
RGFSLHYAAE PTPAECMSDD EDKLLGDFNS EDVRPEGPDN GENSDQKPKV ADWRFGPAQI - 360
WYDILEVPDS GEGFNYGFKT KAASTSSQQQ LKDERRVKSP EDDVEDPSIA DDAFLMVSQL - 420
HWEDDVVWDG NDIKAKVLQK LNSKTNAAGW LPSSGSRTAG AFSQPGKPSM PVGSGSSKQG - 480
SGASSKKAQQ NAQAKPAEAP DDTWYSLFPV ENEELIYYKW EDEVIWDAQQ VSKVPKPKVL - 540
TLDPNDENII LGIPDDIDPS KINKSTGPPP KIKIPHPHVK KSKILLGKAG VINVLAEDTP - 600
PPPPKSPDRD PFNISNDTYY TPKTEPTLRL KVGGNLIQHS TPVVELRAPF VPTHMGPMNV - 660
RAFHRPPLKK YSHGPMAQSI PHPVFPLLKT IAKKAKQREV ERIASGGGDV FFMRNPEDLS - 720
GRDGDIVLAE FCEEHPPLIN QVGMCSKIKN YYKRKAEKDS GPQDYVYGEV AFAHTSPFLG - 780
ILHPGQCIQA IENNMYRAPI YPHKMAHNDF LVIRTRNNYW IRSVNSIYTV GQECPLYEVP - 840
GPNSKRANNF TRDFLQVFIY RLFWKSRDNP RRIRMDDIKQ AFPAHSESSI RKRLKQCADF - 900
KRTGMDSNWW VIKPEFRLPS EEEIRAMVSP EQCCAYFSMI AAEQRLKDAG YGEKFLFAPQ - 960
EDDDEEAQLK LDDEVKVAPW NTTRAYIQAM RGKCLLQLSG PADPTGCGEG FSYVRVPNKP - 1020
TQTKEEQESQ PKRSVTGTDA DLRRLPLQRA KELLRQFKVP EEEIKKLSRW EVIDVVRTLS - 1080
TEKAKAGEEG MDKFSRGNRF SIAEHQERYK EECQRIFDLQ NRVLASSEVL STDEAESSAS - 1140
EESDLEELGK NLENMLSNKK TSTQLSRERE ELERQELLRQ LDEEHGGPSG SGGAKGAKGK - 1200
DDPGQQMLAT NNQGRILRIT RTFRGNDGKE YTRVETVRRQ PVIDAYIKIR TTKDEQFIKQ - 1260
FATLDEQQKE EMKREKRRIQ EQLRRIKRNQ ERERLAQLAQ NQKLQPGGMP TSLGDPKSSG - 1320
GHSHKERDSG YKEVSPSRKK FKLKPDLKLK CGACGQVGHM RTNKACPLYS GMQSSLSQSN - 1380
PSLADDFDEQ SEKEMTMDDD DLVNVDGTKV TLSSKILKRH GGDDGKRRSG SSSGFTLKVP - 1440
RDAMGKKKRR VGGDLHCDYL QRHNKTANRR RTDPVVVLSS ILEIIHNELR SMPDVSPFLF - 1500
PVSAKKVPDY YRVVTKPMDL QTMREYIRQR RYTSREMFLE DLKQIVDNSL IYNGPQSAYT - 1560
LAAQRMFSSC FELLAEREDK LMRLEKAINP LLDDDDQVAL SFIFDKLHSQ IKQLPESWPF - 1620
LKPVNKKQVK DYYTVIKRPM DLETIGKNIE AHRYHSRAEY LADIELIATN CEQYNGSDTR - 1680
YTKFSKKILE YAQTQLIEFS EHCGQLENNI AKTQERAREN APEFDEAWGN DDYNFDRGSR - 1740
ASSPGDDYID VEGHGGHASS SNSIHRSMGA EAGSSHTAPA VRKPAPPGPG EVKRGRGRPR - 1800
KQRDPVEEDL QCSTDDEDDD EEEDFQEVSE DENNAASILD QGERINAPAD AMDGMFDPKN - 1860
IKTEIDLEAH QMADESMDVD PNYDPSDFLA MHKQRQSLGE PSSLQGAFTN FLSHEQDDNG - 1920
PYNPAEASTS AASGADLGMD ASMAMQMAPE MPVNTMNNGM GIDDDLDISE SDEEDDGSRV - 1980
RIKKEVFDDG DYALQHQQMG QAASQSQIYM VDSSNEPTTL DYQQPPQLDF QQVQEMEQLQ - 2040
HQVMPPMQSE QLQQQQTPQG DNDYAWTF
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| Functional narrative |
TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Largest component and core scaffold of the complex. Contains N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. The C-terminal Ser/Thr kinase domain phosphorylates histone H2B at 'Ser-33', which may contribute to transcriptional activation during embryogenesis. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Negative regulator of the TATA box-binding activity of Tbp.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 11 - 77 | | Length: | 67 | | Region sequence: |
EGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLE
VIDLKEA | | Modification type: | Fragment
Native
| | PDB: | 1TBA:A | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | | | Functional subclasses: | Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; pH: 7; D2O 5 %; H2O 95 %; protein (sample) 0.7 mM; Varian 600 MHz (spectrometer))
| References:
- Liu D, Ishima R, Tong KI, Bagby S, Kokubo T, Muhandiram DR, Kay LE, Nakatani Y, Ikura M. "Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP." Cell. 1998; 94(5): 573-583. PubMed: 9741622
| Comments:
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| References |
- Liu D, Ishima R, Tong KI, Bagby S, Kokubo T, Muhandiram DR, Kay LE, Nakatani Y, Ikura M. "Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP." Cell. 1998; 94(5): 573-583. PubMed: 9741622
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| Comments |
AV (6-29-2010) PubMed: 9741622
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