DP00094: Tyrosine 3-monooxygenaseFASTA viewXML view

General information
DisProt:DP00094
Name:Tyrosine 3-monooxygenase
Synonym(s):TY3H_RAT
Tyrosine 3-hydroxylase
TH
EC=1.14.16.2
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P04177
UniGene:Rn.11082
SwissProt: TY3H_RAT
TrEMBL:  
NCBI (GI): 136577
Source organism:Rattus norvegicus (Rat)
Sequence length:498
Percent disordered:31%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MPTPSAPSPQ PKGFRRAVSE QDAKQAEAVT SPRFIGRRQS LIEDARKERE AAAAAAAAAV - 60
ASSEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA VKVFETFEAK IHHLETRPAQ - 120
RPLAGSPHLE YFVRFEVPSG DLAALLSSVR RVSDDVRSAR EDKVPWFPRK VSELDKCHHL - 180
VTKFDPDLDL DHPGFSDQVY RQRRKLIAEI AFQYKHGEPI PHVEYTAEEI ATWKEVYVTL - 240
KGLYATHACR EHLEGFQLLE RYCGYREDSI PQLEDVSRFL KERTGFQLRP VAGLLSARDF - 300
LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ DIGLASLGAS - 360
DEEIEKLSTV YWFTVEFGLC KQNGELKAYG AGLLSSYGEL LHSLSEEPEV RAFDPDTAAV - 420
QPYQDQTYQP VYFVSESFND AKDKLRNYAS RIQRPFSVKF DPYTLAIDVL DSPHTIQRSL - 480
EGVQDELHTL AHALSAIS

Region 1: 1-155

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:N-terminal domain
Location:1 - 155
Length:155
Region sequence:

MPTPSAPSPQPKGFRRAVSEQDAKQAEAVTSPRFIGRRQSLIEDARKEREAAAAAAAAAV
ASSEPGNPLEAVVFEERDGNAVLNLLFSLRGTKPSSLSRAVKVFETFEAKIHHLETRPAQ
RPLAGSPHLEYFVRFEVPSGDLAALLSSVRRVSDD

Modification type: Mutant
Native
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Substrate/ligand binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (pH: 7; JASCO J-600 (spectrophotometer))

  2. Circular dichroism (CD) spectroscopy, near-UV (pH: 7; JASCO -600 (spectrophotometer))

  3. Size exclusion/gel filtration chromatography (277 K; pH: 7.5; flow rate 0.5 mL/min; NaCl 150 mM; sodium phosphate 50 mM; Superose 12 HR 10/30 (column))
References:
  1. Daubner SC, Lohse DL, Fitzpatrick PF. "Expression and characterization of catalytic and regulatory domains of rat tyrosine hydroxylase." Protein Sci. 1993; 2(9): 1452-60. PubMed: 8104613
Comments:
 



References

  1. Goodwill KE, Sabatier C, Marks C, Raag R, Fitzpatrick PF, Stevens RC. "Crystal structure of tyrosine hydroxylase at 2.3 A and its implications for inherited neurodegenerative diseases." Nat Struct Biol. 1997; 4(7): 578-85. PubMed: 9228951


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