DP00118: Chromogranin-AFASTA viewXML view

General information
DisProt:DP00118
Name:Chromogranin-A
Synonym(s):CMGA_BOVIN
CgA
Pituitary secretory protein I
SP-I
Vasostatin-1 [cleavage product 1]
Chromostatin [cleavage product 2]
Chromacin [cleavage product 3]
Pancreastatin [cleavage product 4]
WE-14 [cleavage product 5]
Catestatin [cleavage product 6]
First appeared in release:Release 2.0 (02/14/2005)
UniProt:P05059
UniGene:Bt.49630
SwissProt: CMGA_BOVIN
TrEMBL:  
NCBI (GI): 116548
Source organism:Bos taurus (Bovine)
Sequence length:449
Percent disordered:6%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MRSAAVLALL LCAGQVIALP VNSPMNKGDT EVMKCIVEVI SDTLSKPSPM PVSKECFETL - 60
RGDERILSIL RHQNLLKELQ DLALQGAKER THQQKKHSSY EDELSEVLEK PNDQAEPKEV - 120
TEEVSSKDAA EKRDDFKEVE KSDEDSDGDR PQASPGLGPG PKVEEDNQAP GEEEEAPSNA - 180
HPLASLPSPK HPGPQAKEDS EGPSQGPASR EKGLSAEQGR QTEREEEEEK WEEAEAREKA - 240
VPEEESPPTA AFKAPPSLGN KETQRAAPGW PEDGAGKMGA EEAKPPEGKG EWAHSRQEEE - 300
EMARAPQVLF RGGKSGEPEQ EEQLSKEWED AKRWSKMDQL AKELTAEKRL EGEEEEEEDP - 360
DRSMRLSFRA RGYGFRGPGL QLRRGWRPNS REDSVEAGLP LQVRGYPEEK KEEEGSANRR - 420
PEDQELESLS AIEAELEKVA HQLEELRRG



Functional narrative    

Chromogranin A (CgA) is responsible for calcium storage in secretory vesicles and has been suggest as a precursor for several hormones. CgA is a member of the chromogranin/secretogranin protein family. CgA can be located in neuroendocrine and endocrine secretory granules, generally bound to the membrane at a pH of 5.5. If the surrounding pH is raised to 7.5, CgA is released. The serum level of CgA in the blood is a marker for many types of neuroendocrine tumors. It has also become associated with hypertension and other autonomic dysfunctions. The disordered region of CgA interacts with the cell membrane and mediates binding.

Region 1: 407-431

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name: 
Location:407 - 431
Length:25
Region sequence:

PEEKKEEEGSANRRPEDQELESLSA

Modification type: Engineered
Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular recognition effectors
Functional subclasses: Protein-protein binding
Detection methods:
  1. SDS-PAGE gel, Aberrant mobility on (310 K; pH: 7.5; Tris-HCl 0.2 )

  2. Circular dichroism (CD) spectroscopy, near-UV (295 K; pH: 5.5; sodium acetate (salt) 15 mM)

  3. Circular dichroism (CD) spectroscopy, near-UV (295 K; pH: 7.5; Tris-HCl 15 mM)

  4. Sensitivity to proteolysis
References:
  1. Grimes M, Iacangelo A, Eiden LE, Godfrey B, Herbert E. "Chromogranin A: the primary structure deduced from cDNA clones reveals the presence of pairs of basic amino acids." Ann N Y Acad Sci. 1987; 493: 351-378. PubMed: 3473966

  2. Yoo SH, Ferretti JA. "Nature of the pH-induced conformational changes and exposure of the C-terminal region of chromogranin A." FEBS Lett. 1993; 334(3): 373-377. PubMed: 8243650
Comments:
 



Comments


The disordered region is not found within any of the cleavage products.


If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us.


Disprot-footer
Contact us