Annotation for this protein is in progress - please check future releases for more complete information



DP00197: Translation initiation factor IF-3FASTA viewXML view

General information
DisProt:DP00197
Name:Translation initiation factor IF-3
Synonym(s):IF3_ECOLI
Translation initiation factor IF-3, N-terminally processed [cleavage product 1]
Translation initiation factor IF-3S [cleavage product 2]
First appeared in release:Release 2.2 (04/14/2005)
UniProt:P0A707
UniGene: 
SwissProt: IF3_ECOLI
TrEMBL:  
NCBI (GI): 67466073
Source organism:Escherichia coli
Sequence length:180
Percent disordered:7%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MKGGKRVQTA RPNRINGEIR AQEVRLTGLE GEQLGIVSLR EALEKAEEAG VDLVEISPNA - 60
EPPVCRIMDY GKFLYEKSKS SKEQKKKQKV IQVKEIKFRP GTDEGDYQVK LRSLIRFLEE - 120
GDKAKITLRF RGREMAHQQI GMEVLNRVKD DLQELAVVES FPTKIEGRQM IMVLAPKKKQ - 180



Functional narrative    

Translation initiation factor IF-3 is an essential component of the prokaryotic translation apparatus. When it binds to the 30S ribosomal subunit, it shifts the equilibrium between 70S ribosomes and their 50S and 30S subunits and makes the 30S subunits available for initiation of protein synthesis. Another function of translation initiation factor IF-3 is as a translational repressor protein. By binding to its mRNA, it can control the translation of its own gene.

Region 1: 78-89

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:inter-domain linker
Location:78 - 89
Length:12
Region sequence:

SKSSKEQKKKQK

Modification type: Native
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Entropic chain
Functional subclasses: Flexible linkers/spacers
Detection methods:
  1. Nuclear magnetic resonance (NMR) (pH: 6.5; 20mM potassium phosphate)

References:
  1. Moreau M, de Cock E, Fortier PL, Garcia C, Albaret C, Blanquet S, Lallemand JY, Dardel F. "Heteronuclear NMR studies of E. coli translation initiation factor IF3. Evidence that the inter-domain region is disordered in solution." J Mol Biol. 1997; 266(1): 15-22. PubMed: 9054966

Comments:
 



Comments


Disordered residues lie within the regions corresponding to cleavage products 1 and 2, however, there is no experimental evidence indicating that these products are disordered. The N-terminally processed cleavage product 1 differs from the full-length protein by removal of a single initiator methionine and cleavage product 2 has the first 6 residues removed.


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