Annotations for this protein have been verified by the authors of the corresponding papers


DP00206: Fimbrial proteinFASTA viewXML view

General information
DisProt:DP00206
Name:Fimbrial protein
Synonym(s):FMP1_PSEAE
Pilin
Strain P1
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P17838
UniGene: 
SwissProt: FMP1_PSEAE
TrEMBL:  
NCBI (GI): 120420
Source organism:Pseudomonas aeruginosa
Sequence length:157
Percent disordered:26%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MKAAQKGFTL IELMIVVAII GILAAIAIPA YQDYTARAQL SERMTLASGL KTKVSDIFSQ - 60
DGSCPANTAA TAGIEKDTDI NGKYVAKVTT GGTAAASGGC TIVATMKASD VATPLRGKTL - 120
TLTLGNADKG SYTWACTSNA DNKYLPKTCQ TATTTTP



Functional narrative    

The initial stages of Pseudomonas infection depend on the type IV pilus-mediated adherence of the pathogen to the mucosal cells of a susceptible host. Type IV pilin monomers assemble to form fibers called pili that are required for a variety of bacterial functions. Each pilin protein contains a functional receptor-binding site; however, binding sites are only displayed at the tip of the pilus. This region is proposed to be the point of first contact between bacterial and host cells.

Region 1: 35-37 Region 2: 62-64 Region 3: 95-97 Region 4: 108-116 Region 5: 127-132 Region 6: 141-157

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name: 
Location:35 - 37
Length:3
Region sequence:

TAR

Modification type: Engineered
Fragment
PDB: 1HPW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (303 K; pH: 7.2; sodium chloride 100 mM; sodium phosphate 20 mM)
References:
  1. Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, Sykes BD. "Structure of a pilin monomer from Pseudomonas aeruginosa: implications for the assembly of pili." J Biol Chem. 2001; 276(26): 24186-24193. PubMed: 11294863
Comments:
The experimental sequence consisted of seven residues from the cloning vector followed by residues 8 through 157 of SwissProt sequence P17838.


Region 2
Type:Disordered - Extended
Name: 
Location:62 - 64
Length:3
Region sequence:

GSC

Modification type: Engineered
Fragment
PDB: 1HBW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (303 K; pH: 7.2; sodium chloride 100 mM; sodium phosphate 20 mM)
References:
  1. Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, Sykes BD. "Structure of a pilin monomer from Pseudomonas aeruginosa: implications for the assembly of pili." J Biol Chem. 2001; 276(26): 24186-24193. PubMed: 11294863
Comments:
The experimental sequence consisted of seven residues from the cloning vector followed by residues 8 through 157 of SwissProt sequence P17838.


Region 3
Type:Disordered - Extended
Name: 
Location:95 - 97
Length:3
Region sequence:

AAS

Modification type: Engineered
Fragment
PDB: 1HBW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (303 K; pH: 7.2; sodium chloride 100 mM; sodium phosphate 20 mM)
References:
  1. Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, Sykes BD. "Structure of a pilin monomer from Pseudomonas aeruginosa: implications for the assembly of pili." J Biol Chem. 2001; 276(26): 24186-24193. PubMed: 11294863
Comments:
The experimental sequence consisted of seven residues from the cloning vector followed by residues 8 through 157 of SwissProt sequence P17838.


Region 4
Type:Disordered - Extended
Name: 
Location:108 - 116
Length:9
Region sequence:

ASDVATPLR

Modification type: Engineered
Fragment
PDB: 1HBW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (303 K; pH: 7.2; sodium chloride 100 mM; sodium phosphate 20 mM)
References:
  1. Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, Sykes BD. "Structure of a pilin monomer from Pseudomonas aeruginosa: implications for the assembly of pili." J Biol Chem. 2001; 276(26): 24186-24193. PubMed: 11294863
Comments:
The experimental sequence consisted of seven residues from the cloning vector followed by residues 8 through 157 of SwissProt sequence P17838.


Region 5
Type:Disordered - Extended
Name: 
Location:127 - 132
Length:6
Region sequence:

ADKGSY

Modification type: Engineered
Fragment
PDB: 1HBW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (303 K; pH: 7.2; sodium chloride 100 mM; sodium phosphate 20 mM)
References:
  1. Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, Sykes BD. "Structure of a pilin monomer from Pseudomonas aeruginosa: implications for the assembly of pili." J Biol Chem. 2001; 276(26): 24186-24193. PubMed: 11294863
Comments:
The experimental sequence consisted of seven residues from the cloning vector followed by residues 8 through 157 of SwissProt sequence P17838.


Region 6
Type:Disordered - Extended
Name: 
Location:141 - 157
Length:17
Region sequence:

DNKYLPKTCQTATTTTP

Modification type: Engineered
Fragment
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (303 K; pH: 7.2; sodium chloride 100 mM; sodium phosphate 20 mM)
References:
  1. Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, Sykes BD. "Structure of a pilin monomer from Pseudomonas aeruginosa: implications for the assembly of pili." J Biol Chem. 2001; 276(26): 24186-24193. PubMed: 11294863
Comments:
The experimental sequence consisted of seven residues from the cloning vector followed by residues 8 through 157 of SwissProt sequence P17838.

If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us.


Disprot-footer
Contact us