Annotation for this protein is in progress - please check future releases for more complete information



DP00262: Hypoxia-inducible factor 1 alpha [Isoform 1]FASTA viewXML view

General information
DisProt:DP00262
Name:Hypoxia-inducible factor 1 alpha [Isoform 1]
Synonym(s):HIF1A_HUMAN
HIF-1 alpha
HIF1 alpha
ARNT-interacting protein
Member of PAS protein 1
Basic-helix-loop-helix-PAS protein MOP1
First appeared in release:Release 3.0 (02/17/2006)
UniProt:Q16665-1
UniGene:Hs.597216
SwissProt: HIF1A_HUMAN
TrEMBL:  
NCBI (GI): 2498017
Source organism:Homo sapiens (Human)
Sequence length:826
Percent disordered:42%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM - 60
RLTISYLRVR KLLDAGDLDI EDDMKAQMNC FYLKALDGFV MVLTDDGDMI YISDNVNKYM - 120
GLTQFELTGH SVFDFTHPCD HEEMREMLTH RNGLVKKGKE QNTQRSFFLR MKCTLTSRGR - 180
TMNIKSATWK VLHCTGHIHV YDTNSNQPQC GYKKPPMTCL VLICEPIPHP SNIEIPLDSK - 240
TFLSRHSLDM KFSYCDERIT ELMGYEPEEL LGRSIYEYYH ALDSDHLTKT HHDMFTKGQV - 300
TTGQYRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIVCVNY VVSGIIQHDL IFSLQQTECV - 360
LKPVESSDMK MTQLFTKVES EDTSSLFDKL KKEPDALTLL APAAGDTIIS LDFGSNDTET - 420
DDQQLEEVPL YNDVMLPSPN EKLQNINLAM SPLPTAETPK PLRSSADPAL NQEVALKLEP - 480
NPESLELSFT MPQIQDQTPS PSDGSTRQSS PEPNSPSEYC FYVDSDMVNE FKLELVEKLF - 540
AEDTEAKNPF STQDTDLDLE MLAPYIPMDD DFQLRSFDQL SPLESSSASP ESASPQSTVT - 600
VFQQTQIQEP TANATTTTAT TDELKTVTKD RMEDIKILIA SPSPTHIHKE TTSATSSPYR - 660
DTQSRTASPN RAGKGVIEQT EKSHPRSPNV LSVALSQRTT VPEEELNPKI LALQNAQRKR - 720
KMEHDGSLFQ AVGIGTLLQQ PDDHAATTSL SWKRVKGCKS SEQNGMEQKT IILIPSDLAC - 780
RLLGQSMDES GLPQLTSYDC EVNAPIQGSR NLLQGEELLR ALDQVN

Region 2: 403-603 Region 1: 530-698 Region 3: 776-826

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name:N-TAD and inhibitory domain
Location:530 - 698
Length:169
Region sequence:

EFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSAS
PESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHK
ETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQR

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (DTE (buffer) 1 mM; KCl (buffer) 150 mM; NaPi (buffer (pH 7.2)) 25 mM; protein 30 uM)

  2. Analytical ultracentrifugation (283 K; DTT (buffer) 1 mM; KCl (buffer) 150 mM; NaPi (buffer (pH 7.2)) 25 mM)

  3. Fluorescence polarization/anisotropy (283 K; DTT (buffer) 1 mM; HEPES (pH 7.2) 50 mM)

  4. Fluorescence polarization/anisotropy (283 K; DTT 1 mM; NaCl (buffer (0, 90, and 150 mM)); NaPi (pH 7.2) 25 mM)

References:
  1. Sanchez-Puig N, Veprintsev DB, Fersht AR. "Binding of natively unfolded HIF-1alpha ODD domain to p53." Mol Cell. 2005; 17(1): 11-21. PubMed: 15629713

Comments:
 



Region 2
Type:Disordered - Extended
Name:ODD domain
Location:403 - 603
Length:201
Region sequence:

AAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPL
RSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFY
VDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSP
LESSSASPESASPQSTVTVFQ

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (DTE (buffer) 1 mM; KCl (buffer) 150 mM; NaPi (buffer (pH 7.2)) 25 mM; protein 10 uM)

  2. Analytical ultracentrifugation (283 K; DTT (buffer) 1 mM; KCl (buffer) 150 mM; NaPi (buffer (7.2)) 25 mM)

  3. Fluorescence polarization/anisotropy (283 K; DTT (buffer) 1 mM; HEPES (pH 7.2) 50 mM)

  4. Fluorescence polarization/anisotropy (283 K; DTT 1 mM; NaCl (buffer (0, 90, 150 mM)); NaPi (pH 7.2) 25 mM)

References:
  1. Sanchez-Puig N, Veprintsev DB, Fersht AR. "Binding of natively unfolded HIF-1alpha ODD domain to p53." Mol Cell. 2005; 17(1): 11-21. PubMed: 15629713

Comments:
 



Region 3
Type:Disordered
Name:C-terminal activation domain
Location:776 - 826
Length:51
Region sequence:

SDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Modification type: Native
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes:  
Functional subclasses: Transactivation (transcriptional activation)
Detection methods:
  1. Nuclear magnetic resonance (NMR) (298 K; )

References:
  1. Dames SA, Martinez-Yamout M, De Guzman RN, Dyson HJ, Wright PE. "Structural basis for Hif-1 alpha /CBP recognition in the cellular hypoxic response." Proc Natl Acad Sci U S A. 2002; 99(8): 5271-6. PubMed: 11959977

Comments:
 



References

  1. Masson N, Willam C, Maxwell PH, Pugh CW, Ratcliffe PJ. "Independent function of two destruction domains in hypoxia-inducible factor-alpha chains activated by prolyl hydroxylation." Embo J. 2001; 20(18): 5197-206. PubMed: 11566883

  2. Wang GL, Jiang BH, Rue EA, Semenza GL. "Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension." Proc Natl Acad Sci U S A. 1995; 92(12): 5510-4. PubMed: 7539918


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