Annotations for this protein have been verified by the authors of the corresponding papers


DP00275: Yersinia crystallinFASTA viewXML view

General information
DisProt:DP00275
Name:Yersinia crystallin
Synonym(s):Q7CJN0_YERPE
Beta-gamma-crystallin
Putative exported protein
Putative uncharacterized protein
First appeared in release:Release 3.0 (02/17/2006)
UniProt:Q7CJN0
UniGene: 
SwissProt: Q7CJN0_YERPE
TrEMBL:  
NCBI (GI): 123776891
Source organism:Yersinia pestis
Sequence length:806
Percent disordered:24%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MRYNRNHMLT LMFSLLIFDA YANEPKVCFY MDDDYNGESL CAAQGNSVAS IPDKWNDRIS - 60
SISIPHGLVV TVYEDVDFLG ASRSFEADVD LVSDKDLIYL NDNISAFKIK KAVCFYGESN - 120
FTGDSLCLSS GEQFDLYRGN YPERKQSHLV NPLNDEVYSI KIPPGMQTTV YEDDDYNGKY - 180
FVLTEDYTPD DLLIIRMNNK ISSMRVSQDE DFICDRFCSI KKSIKFKIQK SFGSYWIDPR - 240
IKYRDILLDF QLSDTDDYSI KFFDEGLIKV KNYKLFFFDG NEKHGEGFLF NLSDKSSNLS - 300
FLLRFNGGFF QIQFVESLNN EIVYSSPLIG TLFDDANSDV IFDINNVDAN SPIVIDKMVL - 360
TVGKEHNRAE RSTLGLATCW AIPILSIYNY IVQGHCNQAD KFVHNAADFF GSPADKILQI - 420
FGSSLPLPPK NSNETLDDES ALINFMSDVK GTLTYITTNM GEHALTVPAT ALACKASMRE - 480
KILPHLRNRR DLAPGCIDWT LSILTDFTLL FGASIEYWNS KNFGRVIENI VREGEIGSAV - 540
ADVDTASRLI ESVQAHVAEA TEVADIIHLK TAFDFSQLSY VSYVRHTEEE VVSPQSAQAL - 600
PLGRYELALV DYHFIETVPR IRQDGRWVER PDLHFDIEVI SGVPEETDAS RQVLLPIIEA - 660
WQHSYSQQEL LFHASDILPE SSLARYEIEP LLEASRLTSE VAISWLRTSR DDFVYVVVRL - 720
EGEVISLTMA VDININDVGV AGSLTNPDYV ITPNAEGAVR GAGTAAIRAL ADHFKRKGKI - 780
SLVSSVISQP SAIVKKKVGF RFIEEL

Region 1: 21-112 Region 2: 110-215

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:Domain 1
Location:21 - 112
Length:92
Region sequence:

YANEPKVCFYMDDDYNGESLCAAQGNSVASIPDKWNDRISSISIPHGLVVTVYEDVDFLG
ASRSFEADVDLVSDKDLIYLNDNISAFKIKKA

Modification type: Engineered
Fragment
Monomeric
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Metal sponge
Functional subclasses: Metal binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (298 K; Calcium chloride; DTT 1 mM; KCl 150 mM; Tris (pH 7.5) 20 mM)

  2. Circular dichroism (CD) spectroscopy, near-UV (Calcium chloride; NaCl 100 mM; Tris (pH 7.5) 50 mM)
References:
  1. Jobby MK, Sharma Y. "Calcium-binding crystallins from Yersinia pestis. Characterization of two single betagamma-crystallin domains of a putative exported protein." J Biol Chem. 2005; 280(2): 1209-16. PubMed: 15536081
Comments:
 



Region 2
Type:Disordered
Name:Domain 2
Location:110 - 215
Length:106
Region sequence:

KKAVCFYGESNFTGDSLCLSSGEQFDLYRGNYPERKQSHLVNPLNDEVYSIKIPPGMQTT
VYEDDDYNGKYFVLTEDYTPDDLLIIRMNNKISSMRVSQDEDFICD

Modification type: Engineered
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Metal sponge
Functional subclasses: Metal binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (298 K; Calcium chloride; DTT 1 mM; KCl 100 mM; Tris (pH 7.5) 15 mM)
References:
  1. Jobby MK, Sharma Y. "Calcium-binding crystallins from Yersinia pestis. Characterization of two single betagamma-crystallin domains of a putative exported protein." J Biol Chem. 2005; 280(2): 1209-16. PubMed: 15536081

  2. Jobby MK, Sharma Y. "Purification of a crystallin domain of Yersinia crystallin from inclusion bodies and its comparison to native protein from the soluble fraction." Biomed Chromatogr. 2006; 20(9): 956-63. PubMed: 16470515
Comments:
 



References

  1. Parkhill J, Dougan G, James KD, Thomson NR, Pickard D, Wain J, Churcher C, Mungall KL, Bentley SD, Holden MT, Sebaihia M, Baker S, Basham D, Brooks K, Chillingworth T, Connerton P, Cronin A, Davis P, Davies RM, Dowd L, White N, Farrar J, Feltwell T, Hamlin N, Haque A, Hien TT, Holroyd S, Jagels K, Krogh A, Larsen TS, Leather S, Moule S, O'Gaora P, Parry C, Quail M, Rutherford K, Simmonds M, Skelton J, Stevens K, Whitehead S, Barrell BG. "Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Nature. 2001; 413(6858): 848-52. PubMed: 11677608


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