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DP00289: EntI proteinFASTA viewXML view

General information
DisProt:DP00289
Name:EntI protein
Synonym(s):Q47785_ENTFC
Enterocin A immunity protein
EntA-im
Orf2 protein
First appeared in release:Release 3.0 (02/17/2006)
UniProt:Q47785
UniGene: 
SwissProt: Q47785_ENTFC
TrEMBL:  
NCBI (GI): 75428593
Source organism:Enterococcus faecium (Streptococcus faecium)
Sequence length:103
Percent disordered:31%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MKKNAKQIVH ELYNDISISK DPKYSDILEV LQKVYLKLEK QKYELDPSPL INRLVNYLYF - 60
TAYTNKIRFT EYQEELIRNL SEIGRTAGIN GLYRADYGDK SQF

Region 7: 1-2 Region 5: 1-3 Region 3: 1-3 Region 1: 1-3 Region 2: 40-46 Region 6: 82-91 Region 8: 83-103 Region 4: 86-103

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:1 - 3
Length:3
Region sequence:

MKK

Modification type: Native
PDB: 2BL8:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (110 K; PEG 3350 (15 to 25%); diammonium hydrogen citrate 200 mM)
References:
  1. Dalhus B, Johnsen L, Nissen-Meyer J. "Crystallization and preliminary X-ray data investigation of the bacterial enterocin A immunity protein at 1.65 A resolution." Acta Crystallogr D Biol Crystallogr. 2003; 59(Pt 7): 1291-3. PubMed: 12832790

  2. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain A of the native molecule.


Region 2
Type:Disordered
Name: 
Location:40 - 46
Length:7
Region sequence:

KQKYELD

Modification type: Native
PDB: 2BL8:A
Structural/functional type: Function arises from the disordered state
Functional classes: Entropic chain
Functional subclasses: Flexible linkers/spacers
Detection methods:
  1. X-ray crystallography (110 K; diammonium hydrogen citrate 200 mM; PEG 3350 (15 to 25%))
References:
  1. Dalhus B, Johnsen L, Nissen-Meyer J. "Crystallization and preliminary X-ray data investigation of the bacterial enterocin A immunity protein at 1.65 A resolution." Acta Crystallogr D Biol Crystallogr. 2003; 59(Pt 7): 1291-3. PubMed: 12832790

  2. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain A of the native molecule.


Region 3
Type:Disordered
Name: 
Location:1 - 3
Length:3
Region sequence:

MKK

Modification type: Native
PDB: 2BL8:B
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (110 K; diammonium hydrogen citrate 200 mM; PEG 3350 (15 to 25%))
References:
  1. Dalhus B, Johnsen L, Nissen-Meyer J. "Crystallization and preliminary X-ray data investigation of the bacterial enterocin A immunity protein at 1.65 A resolution." Acta Crystallogr D Biol Crystallogr. 2003; 59(Pt 7): 1291-3. PubMed: 12832790

  2. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain B of the native molecule.


Region 4
Type:Disordered
Name: 
Location:86 - 103
Length:18
Region sequence:

TAGINGLYRADYGDKSQF

Modification type: Native
PDB: 2BL8:B
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography (110 K; diammonium hydrogen citrate 200 mM; PEG 3350 (15 to 25%))
References:
  1. Dalhus B, Johnsen L, Nissen-Meyer J. "Crystallization and preliminary X-ray data investigation of the bacterial enterocin A immunity protein at 1.65 A resolution." Acta Crystallogr D Biol Crystallogr. 2003; 59(Pt 7): 1291-3. PubMed: 12832790

  2. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain B of the native molecule.


Region 5
Type:Disordered
Name: 
Location:1 - 3
Length:3
Region sequence:

MKK

Modification type: Native
PDB: 2BL8:C
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (110 K; diammonium hydrogen citrate 200 mM; PEG 3350 (15 to 25%))
References:
  1. Dalhus B, Johnsen L, Nissen-Meyer J. "Crystallization and preliminary X-ray data investigation of the bacterial enterocin A immunity protein at 1.65 A resolution." Acta Crystallogr D Biol Crystallogr. 2003; 59(Pt 7): 1291-3. PubMed: 12832790

  2. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain C of the native molecule.


Region 6
Type:Disordered
Name: 
Location:82 - 91
Length:10
Region sequence:

EIGRTAGING

Modification type: Native
PDB: 2BL8:C
Structural/functional type: Function arises from the disordered state
Functional classes: Entropic chain
Functional subclasses: Flexible linkers/spacers
Detection methods:
  1. X-ray crystallography (110 K; diammonium hydrogen citrate 200 mM; PEG 3350 (15 to 25%))
References:
  1. Dalhus B, Johnsen L, Nissen-Meyer J. "Crystallization and preliminary X-ray data investigation of the bacterial enterocin A immunity protein at 1.65 A resolution." Acta Crystallogr D Biol Crystallogr. 2003; 59(Pt 7): 1291-3. PubMed: 12832790

  2. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain C of the native molecule.


Region 7
Type:Disordered
Name: 
Location:1 - 2
Length:2
Region sequence:

MK

Modification type: Mutant
PDB: 2BL7:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (298 K; PEG 3350 (15 to 30% (w/v)); protein (3 to 5 mg/ml) 2 ul; sodium tartrate 200 mM)
References:
  1. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain A of the mutant molecule. The mutations included two methionines being inserted in place of lysine 31 and lysine 80.




Region 8
Type:Disordered
Name: 
Location:83 - 103
Length:21
Region sequence:

IGRTAGINGLYRADYGDKSQF

Modification type: Mutant
PDB: 2BL7:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography (298 K; PEG 3350 (15 to 30% (w/v)); protein (3 to 5 mg/ml) 2 ul; sodium tartrate 200 mM)
References:
  1. Johnsen L, Dalhus B, Leiros I, Nissen-Meyer J. "1.6-Angstroms crystal structure of EntA-im. A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A." J Biol Chem. 2005; 280(19): 19045-50. PubMed: 15753083
Comments:
This region was disordered in chain A of the mutant molecule. The mutations included two methionines being inserted in place of lysine 31 and lysine 80.



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