| General information | | DisProt: | DP00342 | | Name: | Colicin-E9 | | Synonym(s): | CEA9_ECOLX
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P09883 | | UniGene: | | | SwissProt: | CEA9_ECOLX | | TrEMBL: | | | NCBI (GI): | 1705745 | | Source organism: | Escherichia coli | | Sequence length: | 582 | | Percent disordered: | 14% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS GSGIHWGGGS - 60
GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA GGLAVSISAS ELSAAIAGII - 120
AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP NMMSKIVTSL PADDITESPV SSLPLDKATV - 180
NVNVRVVDDV KDERQNISVV SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP - 240
AVQTLSPGVT NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR - 300
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ VYNSRKSELD - 360
AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR AQTDVNNKQA AFDAAAKEKS - 420
DADAALSAAQ ERRKQKENKE KDAKDKLDKE SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA - 480
PIPDRIADKL RDKEFKSFDD FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ - 540
VGGRKVYELH HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK
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| Functional narrative |
Colicin E9 is a protein toxin that targets E.Coli and other related bacteria by entering the target cell through the use of its translocation function located on the N-terminus. The N-terminus of this protein is predicted to be disordered in the first 83 residues. This protein toxin functions as an endonuclease and is active on both double and single stranded DNA with the killing function located on the C-terminus and occurs through the enzymatic cleavage of nucleic acids. The C-terminus is not predicted to be disordered. This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 83 | | Length: | 83 | | Region sequence: |
MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNPWGGGSGSGIHWGGGS
GRGNGGGNGNSGGGSGTGGNLSA | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (288 K; pH: 6.2; H2O/2H2O (90/10 %); Potassium Phosphate Buffer 50 mM; sodium azide (w/v) 0.01 %)
| References:
- Collins ES, Whittaker SB, Tozawa K, MacDonald C, Boetzel R, Penfold CN, Reilly A, Clayden NJ, Osborne MJ, Hemmings AM, Kleanthous C, James R, Moore GR. "Structural dynamics of the membrane translocation domain of colicin E9 and its interaction with TolB." J Mol Biol. 2002; 318(3): 787-804. PubMed: 12054823
| Comments:N-terminus responsible for translocation of the cytotoxic domain into the target cell.
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| References |
- Tozawa K, Macdonald CJ, Penfold CN, James R, Kleanthous C, Clayden NJ, Moore GR. "Clusters in an intrinsically disordered protein create a protein-binding site: the TolB-binding region of colicin E9." Biochemistry. 2005; 44(34): 11496-507. PubMed: 16114886
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