Annotation for this protein is in progress - please check future releases for more complete information



DP00345: LeukotoxinFASTA viewXML view

General information
DisProt:DP00345
Name:Leukotoxin
Synonym(s):LKA1A_PASHA
Lkt
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P16535
UniGene: 
SwissProt: LKA1A_PASHA
TrEMBL:  
NCBI (GI): 126356
Source organism:Pasteurella haemolytica (Mannheimia haemolytica)
Sequence length:953
Percent disordered:7%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MGTRLTTLSN GLKNTLTATK SGLHKAGQSL TQAGSSLKTG AKKIILYIPQ NYQYDTEQGN - 60
GLQDLVKAAE ELGIEVQREE RNNIATAQTS LGTIQTAIGL TERGIVLSAP QIDKLLQKTK - 120
AGQALGSAES IVQNANKAKT VLSGIQSILG SVLAGMDLDE ALQNNSNQHA LAKAGLELTN - 180
SLIENIANSV KTLDEFGEQI SQFGSKLQNI KGLGTLGDKL KNIGGLDKAG LGLDVISGLL - 240
SGATAALVLA DKNASTAKKV GAGFELANQV VGNITKAVSS YILAQRVAAG LSSTGPVAAL - 300
IASTVSLAIS PLAFAGIADK FNHAKSLESY AERFKKLGYD GDNLLAEYQR GTGTIDASVT - 360
AINTALAAIA GGVSAAAAGS VIASPIALLV SGITGVISTI LQYSKQAMFE HVANKIHNKI - 420
VEWEKNNHGK NYFENGYDAR YLANLQDNMK FLLNLNKELQ AERVIAITQQ QWDNNIGDLA - 480
GISRLGEKVL SGKAYVDAFE EGKHIKADKL VQLDSANGII DVSNSGKAKT QHILFRTPLL - 540
TPGTEHRERV QTGKYEYITK LNINRVDSWK ITDGAASSTF DLTNVVQRIG IELDNAGNVT - 600
KTKETKIIAK LGEGDDNVFV GSGTTEIDGG EGYDRVHYSR GNYGALTIDA TKETEQGSYT - 660
VNRFVETGKA LHEVTSTHTA LVGNREEKIE YRHSNNQHHA GYYTKDTLKA VEEIIGTSHN - 720
DIFKGSKFND AFNGGDGVDT IDGNDGNDRL FGGKGDDILD GGNGDDFIDG GKGNDLLHGG - 780
KGDDIFVHRK GDGNDIITDS DGNDKLSFSD SNLKDLTFEK VKHNLVITNS KKEKVTIQNW - 840
FREADFAKEV PNYKATKDEK IEEIIGQNGE RITSKQVDDL IAKGNGKITQ DELSKVVDNY - 900
ELLKHSKNVT NSLDKLISSV SAFTSSNDSR NVLVAPTSML DQSLSSLQFA RAA



Functional narrative    

Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca2+ and lysis of the host cell. This leukotoxin is a major contributor to the pathogenesis of lung injury in ovine pneumonic pasteurellosis. It has also week hemolytic activity.

Region 1: 884-953

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:884 - 953
Length:70
Region sequence:

GNGKITQDELSKVVDNYELLKHSKNVTNSLDKLISSVSAFTSSNDSRNVLVAPTSMLDQS
LSSLQFARAA

Modification type: Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular recognition effectors
Functional subclasses: Protein-protein binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (296 K; pH: 7; beta-mercaptoethanol (pH 7) 7 mM; NaCl 200 mM; NaP 20 mM)

References:
  1. Zhang F, Yin Y, Arrowsmith CH, Ling V. "Secretion and circular dichroism analysis of the C-terminal signal peptides of HlyA and LktA." Biochemistry. 1995; 34(13): 4193-201. PubMed: 7703231

Comments:
 


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