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DP00384: High mobility group protein B1FASTA viewXML view

General information
DisProt:DP00384
Name:High mobility group protein B1
Synonym(s):HMGB1_MOUSE
High mobility group protein 1
HMG-1
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P63158
UniGene:Mm.207047
SwissProt: HMGB1_MOUSE
TrEMBL:  
NCBI (GI): 52783747
Source organism:Mus musculus (Mouse)
Sequence length:215
Percent disordered:28%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF - 60
EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL - 120
SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK - 180
SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE



Functional narrative    

Binds preferentially single-stranded DNA and unwinds double stranded DNA. Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Region 1: 2-4 Region 2: 82-90 Region 3: 167-185 Region 4: 186-215

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:2 - 4
Length:3
Region sequence:

GKG

Modification type: Native
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Protein-DNA binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV

  2. Nuclear magnetic resonance (NMR) (293 K; DTT 10 mM; NaCl 150 mM; Phosphate buffer (pH 5.0) 10 mM)

References:
  1. Knapp S, Muller S, Digilio G, Bonaldi T, Bianchi ME, Musco G. "The long acidic tail of high mobility group box 1 (HMGB1) protein forms an extended and flexible structure that interacts with specific residues within and between the HMG boxes." Biochemistry. 2004; 43(38): 11992-7. PubMed: 15379539

Comments:
 



Region 2
Type:Disordered
Name: 
Location:82 - 90
Length:9
Region sequence:

KGETKKKFK

Modification type: Native
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Protein-DNA binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV

  2. Nuclear magnetic resonance (NMR) (293 K; DTT 10 mM; NaCl 150 mM; Phosphate buffer (pH 5.0) 10 mM)

References:
  1. Knapp S, Muller S, Digilio G, Bonaldi T, Bianchi ME, Musco G. "The long acidic tail of high mobility group box 1 (HMGB1) protein forms an extended and flexible structure that interacts with specific residues within and between the HMG boxes." Biochemistry. 2004; 43(38): 11992-7. PubMed: 15379539

Comments:
 



Region 3
Type:Disordered
Name: 
Location:167 - 185
Length:19
Region sequence:

KPDAAKKGVVKAEKSKKKK

Modification type: Native
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Protein-DNA binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV

  2. Nuclear magnetic resonance (NMR) (293 K; DTT 10 mM; NaCl 150 mM; Phosphate buffer (pH 5.0) 10 mM)

References:
  1. Knapp S, Muller S, Digilio G, Bonaldi T, Bianchi ME, Musco G. "The long acidic tail of high mobility group box 1 (HMGB1) protein forms an extended and flexible structure that interacts with specific residues within and between the HMG boxes." Biochemistry. 2004; 43(38): 11992-7. PubMed: 15379539

Comments:
 



Region 4
Type:Disordered
Name:acidic tail
Location:186 - 215
Length:30
Region sequence:

EEEDDEEDEEDEEEEEEEEDEDEEEDDDDE

Modification type: Native
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Protein-DNA binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV

  2. Nuclear magnetic resonance (NMR) (293 K; DTT 10 mM; NaCl 150 mM; Phosphate buffer (pH 5.0) 10 mM)

References:
  1. Knapp S, Muller S, Digilio G, Bonaldi T, Bianchi ME, Musco G. "The long acidic tail of high mobility group box 1 (HMGB1) protein forms an extended and flexible structure that interacts with specific residues within and between the HMG boxes." Biochemistry. 2004; 43(38): 11992-7. PubMed: 15379539

Comments:
 



Comments


Additional UniGene ID: Mm.313345


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