| General information | | DisProt: | DP00389 | | Name: | Hemolysin, chromosomal | | Synonym(s): | HLYAC_ECOLX
HlyA
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P09983 | | UniGene: | | | SwissProt: | HLYAC_ECOLX | | TrEMBL: | | | NCBI (GI): | 123187 | | Source organism: | Escherichia coli | | Sequence length: | 1023 | | Percent disordered: | 6% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MPTITAAQIK STLQSAKQSA ANKLHSAGQS TKDALKKAAE QTRNAGNRLI LLIPKDYKGQ - 60
GSSLNDLVRT ADELGIEVQY DEKNGTAITK QVFGTAEKLI GLTERGVTIF APQLDKLLQK - 120
YQKAGNKLGG SAENIGDNLG KAGSVLSTFQ NFLGTALSSM KIDELIKKQK SGGNVSSSEL - 180
AKASIELINQ LVDTAASLNN VNSFSQQLNK LGSVLSNTKH LNGVGNKLQN LPNLDNIGAG - 240
LDTVSGILSA ISASFILSNA DADTGTKAAA GVELTTKVLG NVGKGISQYI IAQRAAQGLS - 300
TSAAAAGLIA SVVTLAISPL SFLSIADKFK RANKIEEYSQ RFKKLGYDGD SLLAAFHKET - 360
GAIDASLTRI STVLASVSSG ISAAATTSLV GAPVSALVGA VTGIISGILE ASKQAMFEHV - 420
ASKMADVIAE WEKKHGKNYF ENGYDARHAA FLEDNFKILS QYNKEYSVER SVLITQQHWD - 480
TLIGELAGVT RNGDKTLSGK SYIDYYEEGK RLEKKPDEFQ KQVFDPLKGN IDLSDSKSST - 540
LLKFVTPLLT PGEEIRERRQ SGKYEYITEL LVKGVDKWTV KGVQDKGSVY DYSNLIQHAS - 600
VGNNQYREIR IESHLGDGDD KVFLSAGSAN IYAGKGHDVV YYDKTDTGYL TIDGTKATEA - 660
GNYTVTRVLG GDVKVLQEVV KEQEVSVGKR TEKTQYRSYE FTHINGKNLT ETDNLYSVEE - 720
LIGTTRADKF FGSKFADIFH GADGDDHIEG NDGNDRLYGD KGNDTLSGGN GDDQLYGGDG - 780
NDKLIGGAGN NYLNGGDGDD ELQVQGNSLA KNVLSGGKGN DKLYGSEGAD LLDGGEGNDL - 840
LKGGYGNDIY RYLSGYGHHI IDDDGGKDDK LSLADIDFRD VAFRREGNDL IMYKAEGNVL - 900
SIGHKNGITF KNWFEKESGD ISNHQIEQIF DKDGRVITPD SLKKALEYQQ SNNKASYVYG - 960
NDALAYGSQG NLNPLINEIS KIISAAGNFD VKEERAAASL LQLSGNASDF SYGRNSITLT - 1020
ASA
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| Functional narrative |
Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture by mechanisms not clearly defined.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | C-terminal | | Location: | 963 - 1023 | | Length: | 61 | | Region sequence: |
ALAYGSQGNLNPLINEISKIISAAGNFDVKEERAAASLLQLSGNASDFSYGRNSITLTAS
A | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Unknown
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (296 K; pH: 7; beta-mercaptoethanol 7 mM; NaCl 200 mM; NaP 20 mM)
- Circular dichroism (CD) spectroscopy, far-UV (296 K; pH: 5.5; beta-mercaptoethanol 10 mM; NaCl 250 mM; NaPi 25 mM)
| References:
- Zhang F, Yin Y, Arrowsmith CH, Ling V. "Secretion and circular dichroism analysis of the C-terminal signal peptides of HlyA and LktA." Biochemistry. 1995; 34(13): 4193-201. PubMed: 7703231
| Comments:The 61 bp C-terminus disordered region published in the Zhang paper contains discrepancies when compared to the SwisProt sequence. The SwisProt sequence at regions 964, 965, 970 and 996 are L, A, G, A, compared to the disorder paper sequence of S, T, D, S respectively.
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| References |
- Felmlee T, Pellett S, Welch RA. "Nucleotide sequence of an Escherichia coli chromosomal hemolysin." J Bacteriol. 1985; 163(1): 94-105. PubMed: 3891743
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