Annotation for this protein is in progress - please check future releases for more complete information



DP00395: Calmodulin-sensitive adenylate cyclaseFASTA viewXML view

General information
DisProt:DP00395
Name:Calmodulin-sensitive adenylate cyclase
Synonym(s):CYAA_BACAN
EC 4.6.1.1
ATP pyrophosphate-lyase
Adenylyl cyclase
Anthrax edema toxin adenylate cyclase component
Edema factor
EF
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P40136
UniGene: 
SwissProt: CYAA_BACAN
TrEMBL:  
NCBI (GI): 729244
Source organism:Bacillus anthracis
Sequence length:800
Percent disordered:2%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MTRNKFIPNK FSIISFSVLL FAISSSQAIE VNAMNEHYTE SDIKRNHKTE KNKTEKEKFK - 60
DSINNLVKTE FTNETLDKIQ QTQDLLKKIP KDVLEIYSEL GGEIYFTDID LVEHKELQDL - 120
SEEEKNSMNS RGEKVPFASR FVFEKKRETP KLIINIKDYA INSEQSKEVY YEIGKGISLD - 180
IISKDKSLDP EFLNLIKSLS DDSDSSDLLF SQKFKEKLEL NNKSIDINFI KENLTEFQHA - 240
FSLAFSYYFA PDHRTVLELY APDMFEYMNK LEKGGFEKIS ESLKKEGVEK DRIDVLKGEK - 300
ALKASGLVPE HADAFKKIAR ELNTYILFRP VNKLATNLIK SGVATKGLNV HGKSSDWGPV - 360
AGYIPFDQDL SKKHGQQLAV EKGNLENKKS ITEHEGEIGK IPLKLDHLRI EELKENGIIL - 420
KGKKEIDNGK KYYLLESNNQ VYEFRISDEN NEVQYKTKEG KITVLGEKFN WRNIEVMAKN - 480
VEGVLKPLTA DYDLFALAPS LTEIKKQIPQ KEWDKVVNTP NSLEKQKGVT NLLIKYGIER - 540
KPDSTKGTLS NWQKQMLDRL NEAVKYTGYT GGDVVNHGTE QDNEEFPEKD NEIFIINPEG - 600
EFILTKNWEM TGRFIEKNIT GKDYLYYFNR SYNKIAPGNK AYIEWTDPIT KAKINTIPTS - 660
AEFIKNLSSI RRSSNVGVYK DSGDKDEFAK KESVKKIAGY LSDYYNSANH IFSQEKKRKI - 720
SIFRGIQAYN EIENVLKSKQ IAPEYKNYFQ YLKERITNQV QLLLTHQKSN IEFKLLYKQL - 780
NFTENETDNF EVFQKIIDEK



Functional narrative    

One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. EF is a calmodulin-dependent adenylyl cyclase that, when associated with PA, causes edema. EF is not toxic by itself and it is required for the survival of germinated spores within macrophages at the early stages of infection. Provokes dramatic elevation of intracellular cAMP levels in the host.

Region 1: 578-591

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:Switch B
Location:578 - 591
Length:14
Region sequence:

GTEQDNEEFPEKDN

Modification type: Complex
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Modification site
Functional subclasses: Substrate/ligand binding
Detection methods:
  1. X-ray crystallography (Citric acid (pH 5.65) 100 mM; Glycerol (15%); MgCl2 2 mM; NaCl (pH 7.5) 50 mM; NiSO4 220 mM; PEG 550MME (11.5%); protein 40 mg/ml; Tris-HCl 20 mM)

References:
  1. Drum CL, Yan SZ, Bard J, Shen YQ, Lu D, Soelaiman S, Grabarek Z, Bohm A, Tang WJ. "Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin." Nature. 2002; 415(6870): 396-402. PubMed: 11807546

Comments:
 


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