| General information | | DisProt: | DP00395 | | Name: | Calmodulin-sensitive adenylate cyclase | | Synonym(s): | CYAA_BACAN
EC 4.6.1.1
ATP pyrophosphate-lyase
Adenylyl cyclase
Anthrax edema toxin adenylate cyclase component
Edema factor
EF
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P40136 | | UniGene: | | | SwissProt: | CYAA_BACAN | | TrEMBL: | | | NCBI (GI): | 729244 | | Source organism: | Bacillus anthracis | | Sequence length: | 800 | | Percent disordered: | 2% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MTRNKFIPNK FSIISFSVLL FAISSSQAIE VNAMNEHYTE SDIKRNHKTE KNKTEKEKFK - 60
DSINNLVKTE FTNETLDKIQ QTQDLLKKIP KDVLEIYSEL GGEIYFTDID LVEHKELQDL - 120
SEEEKNSMNS RGEKVPFASR FVFEKKRETP KLIINIKDYA INSEQSKEVY YEIGKGISLD - 180
IISKDKSLDP EFLNLIKSLS DDSDSSDLLF SQKFKEKLEL NNKSIDINFI KENLTEFQHA - 240
FSLAFSYYFA PDHRTVLELY APDMFEYMNK LEKGGFEKIS ESLKKEGVEK DRIDVLKGEK - 300
ALKASGLVPE HADAFKKIAR ELNTYILFRP VNKLATNLIK SGVATKGLNV HGKSSDWGPV - 360
AGYIPFDQDL SKKHGQQLAV EKGNLENKKS ITEHEGEIGK IPLKLDHLRI EELKENGIIL - 420
KGKKEIDNGK KYYLLESNNQ VYEFRISDEN NEVQYKTKEG KITVLGEKFN WRNIEVMAKN - 480
VEGVLKPLTA DYDLFALAPS LTEIKKQIPQ KEWDKVVNTP NSLEKQKGVT NLLIKYGIER - 540
KPDSTKGTLS NWQKQMLDRL NEAVKYTGYT GGDVVNHGTE QDNEEFPEKD NEIFIINPEG - 600
EFILTKNWEM TGRFIEKNIT GKDYLYYFNR SYNKIAPGNK AYIEWTDPIT KAKINTIPTS - 660
AEFIKNLSSI RRSSNVGVYK DSGDKDEFAK KESVKKIAGY LSDYYNSANH IFSQEKKRKI - 720
SIFRGIQAYN EIENVLKSKQ IAPEYKNYFQ YLKERITNQV QLLLTHQKSN IEFKLLYKQL - 780
NFTENETDNF EVFQKIIDEK
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| Functional narrative |
One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. EF is a calmodulin-dependent adenylyl cyclase that, when associated with PA, causes edema. EF is not toxic by itself and it is required for the survival of germinated spores within macrophages at the early stages of infection. Provokes dramatic elevation of intracellular cAMP levels in the host.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | Switch B | | Location: | 578 - 591 | | Length: | 14 | | Region sequence: |
GTEQDNEEFPEKDN | | Modification type: | Complex
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Modification site
| | Functional subclasses: | Substrate/ligand binding
| Detection methods:
- X-ray crystallography (Citric acid (pH 5.65) 100 mM; Glycerol (15%); MgCl2 2 mM; NaCl (pH 7.5) 50 mM; NiSO4 220 mM; PEG 550MME (11.5%); protein 40 mg/ml; Tris-HCl 20 mM)
| References:
- Drum CL, Yan SZ, Bard J, Shen YQ, Lu D, Soelaiman S, Grabarek Z, Bohm A, Tang WJ. "Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin." Nature. 2002; 415(6870): 396-402. PubMed: 11807546
| Comments:
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