| General information | | DisProt: | DP00404_A002 | | Name: | Sulfotransferase family cytosolic 2B member 1 [Isoform 2 (SULT2B1a)(A)] | | Synonym(s): | ST2B1_HUMAN
Sulfotransferase 2B1
EC 2.8.2.2
Alcohol sulfotransferase
Hydroxysteroid sulfotransferase 2
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | O00204-2 | | UniGene: | Hs.369331 | | SwissProt: | ST2B1_HUMAN | | TrEMBL: | | | NCBI (GI): | 5701328 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 350 | | Percent disordered: | 18% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MASPPPFHSQ KLPGEYFRYK GVPFPVGLYS LESISLAENT QDVRDDDIFI ITYPKSGTTW - 60
MIEIICLILK EGDPSWIRSV PIWERAPWCE TIVGAFSLPD QYSPRLMSSH LPIQIFTKAF - 120
FSSKAKVIYM GRNPRDVVVS LYHYSKIAGQ LKDPGTPDQF LRDFLKGEVQ FGSWFDHIKG - 180
WLRMKGKDNF LFITYEELQQ DLQGSVERIC GFLGRPLGKE ALGSVVAHST FSAMKANTMS - 240
NYTLLPPSLL DHRRGAFLRK GVCGDWKNHF TVAQSEAFDR AYRKQMRGMP TFPWDEDPEE - 300
DGSPDPEPSP EPEPKPSLEP NTSLEREPRP NSSPSPSPGQ ASETPHPRPS
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| Functional narrative |
Catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 11 | | Length: | 11 | | Region sequence: |
MASPPPFHSQK | | Modification type: | Complex
Isoform
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (pH: 9; CHES 0.1 M; Li2SO4 0.2 M; sodium tartrate (0.8 to 1.0 M))
| References:
- Lee KA, Fuda H, Lee YC, Negishi M, Strott CA, Pedersen LC. "Crystal structure of human cholesterol sulfotransferase (SULT2B1b) in the presence of pregnenolone and 3'-phosphoadenosine 5'-phosphate. Rationale for specificity differences between prototypical SULT2A1 and the SULT2BG1 isoforms." J Biol Chem. 2003; 278(45): 44593-9. PubMed: 12923182
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 299 - 350 | | Length: | 52 | | Region sequence: |
EEDGSPDPEPSPEPEPKPSLEPNTSLEREPRPNSSPSPSPGQASETPHPRPS | | Modification type: | Complex
Isoform
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (pH: 9; CHES 0.1 M; Li2SO4 0.2 M; sodium tartrate (0.8 to 1.0 M))
| References:
- Lee KA, Fuda H, Lee YC, Negishi M, Strott CA, Pedersen LC. "Crystal structure of human cholesterol sulfotransferase (SULT2B1b) in the presence of pregnenolone and 3'-phosphoadenosine 5'-phosphate. Rationale for specificity differences between prototypical SULT2A1 and the SULT2BG1 isoforms." J Biol Chem. 2003; 278(45): 44593-9. PubMed: 12923182
| Comments:
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| References |
- Her C, Wood TC, Eichler EE, Mohrenweiser HW, Ramagli LS, Siciliano MJ, Weinshilboum RM. "Human hydroxysteroid sulfotransferase SULT2B1: two enzymes encoded by a single chromosome 19 gene." Genomics. 1998; 53(3): 284-95. PubMed: 9799594
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| Comments |
The sequence for Isoform II can be created using "Features which should be applied to build the isoform sequence: VSP_012510" found in the SwissProt entry for Isoform I (seen here O00204).
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