| General information | | DisProt: | DP00432 | | Name: | Non-histone chromosomal protein 6A | | Synonym(s): | NHP6A_YEAST
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P11632 | | UniGene: | | | SwissProt: | NHP6A_YEAST | | TrEMBL: | | | NCBI (GI): | 128182 | | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | | Sequence length: | 93 | | Percent disordered: | 18% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MVTPREPKKR TTRKKKDPNA PKRALSAYMF FANENRDIVR SENPDITFGQ VGKKLGEKWK - 60
ALTPEEKQPY EAKAQADKKR YESEKELYNA TLA
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| Functional narrative |
DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6B.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 7 | | Length: | 7 | | Region sequence: |
MVTPREP | | Modification type: | | | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (293 K; pH: 5.5; NaCl 100 mM; NaPO4 10 mM)
- Nuclear magnetic resonance (NMR) (310 K; pH: 6; NaCl 50 mM; NaPO4 10 mM)
| References:
- Allain FH, Yen YM, Masse JE, Schultze P, Dieckmann T, Johnson RC, Feigon J. "Solution structure of the HMG protein NHP6A and its interaction with DNA reveals the structural determinants for non-sequence-specific binding." EMBO J. 1999; 18(9): 2563-79. PubMed: 10228169
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 8 - 17 | | Length: | 10 | | Region sequence: |
KKRTTRKKKD | | Modification type: | | | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (293 K; pH: 5.5; NaCl 100 mM; NaPO4 10 mM)
- Nuclear magnetic resonance (NMR) (310 K; pH: 6; NaCl 100 mM; NaPO4 10 mM)
| References:
- Allain FH, Yen YM, Masse JE, Schultze P, Dieckmann T, Johnson RC, Feigon J. "Solution structure of the HMG protein NHP6A and its interaction with DNA reveals the structural determinants for non-sequence-specific binding." EMBO J. 1999; 18(9): 2563-79. PubMed: 10228169
| Comments:
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