| General information | | DisProt: | DP00433 | | Name: | Lactose operon repressor | | Synonym(s): | LACI_ECOLI
Lac repressor
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P03023 | | UniGene: | | | SwissProt: | LACI_ECOLI | | TrEMBL: | | | NCBI (GI): | 33112645 | | Source organism: | Escherichia coli | | Sequence length: | 360 | | Percent disordered: | 17% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ - 60
SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS - 120
GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ - 180
QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT - 240
AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS - 300
VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ - 360
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| Functional narrative |
Repressor of the lactose operon. Binds allolactose as an inducer.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 2 | | Length: | 2 | | Region sequence: |
MK | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; KCl 0.4 M; potassium phosphate (pH 4.5) 0.06 M; NaN3 (w/v) 0.02 %; H2O/2H2O (95%/5% (v/v)))
- Nuclear magnetic resonance (NMR) (298 K; 2H2O 99.9 %; KCl 0.4 M; NaN3 (w/v) 0.02 %; potassium phosphate (pH 4.5) 0.06 M)
| References:
- Slijper M, Bonvin AM, Boelens R, Kaptein R. "Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: change of tertiary structure upon binding to the lac operator." J Mol Biol. 1996; 259(4): 761-73. PubMed: 8683581
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 25 - 31 | | Length: | 7 | | Region sequence: |
NQASHVS | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; H2O/2H2O (95%/5% (v/v)); KCl 0.4 M; NaN3 (w/v) 0.02 %; potassium phosphate (pH 4.5) 0.06 M)
- Nuclear magnetic resonance (NMR) (298 K; 2H2O 99.9 %; KCl 0.4 M; NaN3 (w/v) 0.02 %; potassium phosphate (pH 4.5) 0.06 M)
| References:
- Slijper M, Bonvin AM, Boelens R, Kaptein R. "Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: change of tertiary structure upon binding to the lac operator." J Mol Biol. 1996; 259(4): 761-73. PubMed: 8683581
| Comments:
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| Region 3 | | Type: | Disordered | | Name: | | | Location: | 50 - 56 | | Length: | 7 | | Region sequence: |
NRVAQQL | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; H2O/2H2O (95%/5% (v/v)); KCl 0.4 M; NaN3 (w/v) 0.02 %; potassium phosphate (pH 4.5) 0.06 M)
- Nuclear magnetic resonance (NMR) (298 K; 2H2O 99.9 %; KCl 0.4 M; NaN3 (w/v) 0.02 %; potassium phosphate (pH 4.5) 0.06 M)
| References:
- Slijper M, Bonvin AM, Boelens R, Kaptein R. "Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: change of tertiary structure upon binding to the lac operator." J Mol Biol. 1996; 259(4): 761-73. PubMed: 8683581
| Comments:
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| Region 4 | | Type: | Disordered | | Name: | hinge region | | Location: | 46 - 62 | | Length: | 17 | | Region sequence: |
NYIPNRVAQQLAGKQSL | | Modification type: | | | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
Entropic chain
| | Functional subclasses: | Flexible linkers/spacers
| Detection methods:
- X-ray crystallography
| References:
- Kercher MA, Lu P, Lewis M. "Lac repressor-operator complex." Curr Opin Struct Biol. 1997; 7(1): 76-85. PubMed: 9032054
| Comments:
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| Region 5 | | Type: | Disordered | | Name: | HTH region | | Location: | 6 - 25 | | Length: | 20 | | Region sequence: |
LYDVAEYAGVSYQTVSRVVN | | Modification type: | | | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- X-ray crystallography
| References:
- Kercher MA, Lu P, Lewis M. "Lac repressor-operator complex." Curr Opin Struct Biol. 1997; 7(1): 76-85. PubMed: 9032054
| Comments:
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| Region 6 | | Type: | Disordered | | Name: | | | Location: | 1 - 61 | | Length: | 61 | | Region sequence: |
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQ
S | | Modification type: | | | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- X-ray crystallography (potassium phosphate (pH 5.59); tris buffer (pH 7.4))
| References:
- Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P. "Crystal structure of the lactose operon repressor and its complexes with DNA and inducer." Science. 1996; 271(5253): 1247-54. PubMed: 8638105
| Comments:
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| References |
- Farabaugh PJ. "Sequence of the lacI gene." Nature. 1978; 274(5673): 765-9. PubMed: 355891
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