| General information | | DisProt: | DP00475 | | Name: | H/ACA ribonucleoprotein complex subunit 3 | | Synonym(s): | NOP10_YEAST
Nucleolar protein family A member 3
snoRNP protein NOP10
Nucleolar protein 10
| | First appeared in release: | Release 3.5 (12/22/2006) | | UniProt: | Q6Q547 | | UniGene: | | | SwissProt: | NOP10_YEAST | | TrEMBL: | | | NCBI (GI): | 54036193 | | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | | Sequence length: | 58 | | Percent disordered: | 74% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MHLMYTLGPD GKRIYTLKKV TESGEITKSA HPARFSPDDK YSRQRVTLKK RFGLVPGQ
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| Functional narrative |
In eukaryotes and archaebacteria, pseudouridine modification of ribosomal RNA is mediated by box H/ACA small nucleolar RNPs (snoRNP). Box H/ACA snoRNAs are complexed with four different proteins to form the snoRNPs. In yeast (Saccharomyces cerevisiae), these are Nop10p (6 kDa), Nhp2p (22 kDa), Gar1p (22 kDa), and the putative pseudouridine synthase, Cbf5p (65 kDa). All four proteins of box H/ACA snoRNPs have been shown to be essential for viability. Nop10 was found to directly interact with the pseudouridine synthase (Cbf5p/Nap57 or aCbf5). Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 4 | | Length: | 4 | | Region sequence: |
MHLM | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- Nuclear magnetic resonance (NMR) (288 K; pH: 6.5; 15N, 13C labeled protein; KCL 100 mM; potassium phosphate 10 mM)
| References:
- Khanna M, Wu H, Johansson C, Caizergues-Ferrer M, Feigon J. "Structural study of the H/ACA snoRNP components Nop10p and the 3' hairpin of U65 snoRNA." RNA. 2006; 12(1): 40-52. PubMed: 16373493
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | L2 | | Location: | 17 - 38 | | Length: | 22 | | Region sequence: |
LKKVTESGEITKSAHPARFSPD | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (288 K; pH: 6.5; 15N, 13C labeled protein; KCL 100 mM; ptassium phosphate 10 mM)
| References:
- Khanna M, Wu H, Johansson C, Caizergues-Ferrer M, Feigon J. "Structural study of the H/ACA snoRNP components Nop10p and the 3' hairpin of U65 snoRNA." RNA. 2006; 12(1): 40-52. PubMed: 16373493
| Comments:
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| Region 3 | | Type: | Disordered | | Name: | | | Location: | 42 - 58 | | Length: | 17 | | Region sequence: |
SRQRVTLKKRFGLVPGQ | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
| References:
- Khanna M, Wu H, Johansson C, Caizergues-Ferrer M, Feigon J. "Structural study of the H/ACA snoRNP components Nop10p and the 3' hairpin of U65 snoRNA." RNA. 2006; 12(1): 40-52. PubMed: 16373493
| Comments:
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| References |
- Henras A, Henry Y, Bousquet-Antonelli C, Noaillac-Depeyre J, Gelugne JP, Caizergues-Ferrer M. "Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs." Embo J. 1998; 17(23): 7078-90. PubMed: 9843512
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