Annotations for this protein have been verified by the authors of the corresponding papers


DP00483: Prion proteinFASTA viewXML view

General information
DisProt:DP00483
Name:Prion protein
Synonym(s):Q7LKB1_YEASX
SUP35
Q8TFB8_YEAST (obsolete, replaced by Q7LKB1_YEASX)
First appeared in release:Release 3.1 (03/31/2006)
UniProt:Q8TFB8
UniGene: 
SwissProt: Q8TFB8_YEAST
TrEMBL:  
NCBI (GI): 74622196
Source organism:Saccharomyces cerevisiae (Baker's yeast)
Sequence length:685
Percent disordered:37%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MSDSNQGNNQ QNYQQYSQNG NQQQGNNRYQ GYQAYNAQAQ PAGGYYQNYQ GYSGYQQGGY - 60
QQYNPDAGYQ QQYNPQGGYQ QYNPQGGYQQ QFNPQGGRGN YKNFNYNNNL QGYQAGFQPQ - 120
SQGMSLNDFQ KQQKQAAPKP KKTLKLVSSS GIKLANATKK VDTKPAESDK KEEEKSAETK - 180
EPTKEPTKVE EPVKKEEKPV QTEEKTEEKS ELPKVEDLKI SESTHNTNNA NVTSADALIK - 240
EQEEEVDDEV VNDMFGGKDH VSLIFMGHVD AGKSTMGGNL LYLTGSVDKR TIEKYEREAK - 300
DAGRQGWYLS WVMDTNKEER NDGKTIEVGK AYFETEKRRY TILDAPGHKM YVSEMIGGAS - 360
QADVGVLVIS ARKGEYETGF ERGGQTREHA LLAKTQGVNK MVVVVNKMDD PTVNWSKERY - 420
DQCVSNVSNF LRAIGYNIKT DVVFMPVSGY SGANLKDHVD PKECPWYTGP TLLEYLDTMN - 480
HVDRHINAPF MLPIAAKMKD LGTIVEGKIE SGHIKKGQST LLMPNKTAVE IQNIYNETEN - 540
EVDMAMCGEQ VKLRIKGVEE EDISPGFVLT SPKNPIKSVT KFVAQIAIVE LKSIIAAGFS - 600
CVMHVHTAIE EVHIVKLLHK LEKGTNRKSK KPPAFAKKGM KVIAVLETEA PVCVETYQDY - 660
PQLGRFTLRD QGTTIAIGKI VKIAE

Region 1: 1-253

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:Sup35p-NM
Location:1 - 253
Length:253
Region sequence:

MSDSNQGNNQQNYQQYSQNGNQQQGNNRYQGYQAYNAQAQPAGGYYQNYQGYSGYQQGGY
QQYNPDAGYQQQYNPQGGYQQYNPQGGYQQQFNPQGGRGNYKNFNYNNNLQGYQAGFQPQ
SQGMSLNDFQKQQKQAAPKPKKTLKLVSSSGIKLANATKKVDTKPAESDKKEEEKSAETK
EPTKEPTKVEEPVKKEEKPVQTEEKTEEKSELPKVEDLKISESTHNTNNANVTSADALIK
EQEEEVDDEVVND

Modification type: Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular assembly
Functional subclasses: Unknown
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (pH: 7.4; NaCl 150 mM; potassium phosphate 5 mM)

  2. Dynamic light scattering
References:
  1. Resende CG, Outeiro TF, Sands L, Lindquist S, Tuite MF. "Prion protein gene polymorphisms in Saccharomyces cerevisiae." Mol Microbiol. 2003; 49(4): 1005-17. PubMed: 12890024

  2. Scheibel T, Lindquist SL. "The role of conformational flexibility in prion propagation and maintenance for Sup35p." Nat Struct Biol. 2001; 8(11): 958-62. PubMed: 11685242
Comments:
 



Comments


Per UniProt, entry Q7LKB1_YEASX replaces Q8TFB8_YEAST which is listed as UniProt/SwissProt cross-reference for DP00483.


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