| General information | | DisProt: | DP00490 | | Name: | Growth factor receptor-bound protein 14 | | Synonym(s): | GRB14_RAT
GRB14 adapter protein
| | First appeared in release: | Release 3.2 (05/26/2006) | | UniProt: | O88900 | | UniGene: | Rn.30028 | | SwissProt: | GRB14_RAT | | TrEMBL: | | | NCBI (GI): | 6016156 | | Source organism: | Rattus norvegicus (Rat) | | Sequence length: | 538 | | Percent disordered: | 14% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MTTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP ASDATRRGAM - 60
DRRKAKDLEV QETPSIPNPF PELCCSPLTS VLSAGLFPRS NSRKKQVIKV YSEDETSRAL - 120
EVPSDVTARD VCQLLILKNH YVDDNSWTLF EHLSHTGVER TVEDHELLTE VLSHWVMEED - 180
NKLYLRKNYA KYEFFKNPMY FFPEHMVSFA TEMNGDRSLT QIPQVFLSSN TYPEIHGFLH - 240
AKEQGKKSWK KAYFFLRRSG LYFSTKGTSK EPRHLQFFSE FSTSNVYMSL AGKKKHGAPT - 300
PYGFCFKPTK AGGPRDLKML CAEEDQSRMC WVTAIRLLKY GMQLYQNYMH PSQARSACSS - 360
QSVSPMRSVS ENSLVAMDFS GQKTRVIDNP TEALSVAVEE GLAWRKKGCL RLGNHGSPTA - 420
PSQSSAVNMA LHRSQPWFHH RISRDEAQQL ITRQGPVDGV FLVRDSQSNP RTFVLSMSHG - 480
QKIKHFQIIP VEDDGEVFHT LDDGHTKFTD LIQLVEFYQL NKGVLPCKLK HYCARMAV
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| Functional narrative |
Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor which is then inhibited. The interaction is mediated by the SH2 domain. Binds to the ankyrin repeat region of TNKL via its N-terminus.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | PIR | | Location: | 361 - 435 | | Length: | 75 | | Region sequence: |
QSVSPMRSVSENSLVAMDFSGQKTRVIDNPTEALSVAVEEGLAWRKKGCLRLGNHGSPTA
PSQSSAVNMALHRSQ | | Modification type: | Fragment
Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Substrate/ligand binding
Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (300 K; pH: 5; H2O/2H2O (90/10))
- Stability at thermal extremes
- Small-angle X-ray scattering (SAXS) (NaCl 100 mM; TCEP 1 mM; Tris/HCl (pH 8.0) 50 mM)
- Circular dichroism (CD) spectroscopy, far-UV (293 K; pH: 7; sodium phosphate (buffer) 10 mM)
| References:
- Moncoq K, Broutin I, Craescu CT, Vachette P, Ducruix A, Durand D. "SAXS study of the PIR domain from the Grb14 molecular adaptor: a natively unfolded protein with a transient structure primer?" Biophys J. 2004; 87(6): 4056-64. PubMed: 15465854
- Moncoq K, Broutin I, Larue V, Perdereau D, Cailliau K, Browaeys-Poly E, Burnol AF, Ducruix A. "The PIR domain of Grb14 is an intrinsically unstructured protein: implication in insulin signaling." FEBS Lett. 2003; 554(3): 240-6. PubMed: 14623073
| Comments:Residues 399-407 may be transiently structured, and may be important in the binding of Grb14 binding to its partner via a structural transition.
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| References |
- Kasus-Jacobi A, Perdereau D, Auzan C, Clauser E, Van Obberghen E, Mauvais-Jarvis F, Girard J, Burnol AF. "Identification of the rat adapter Grb14 as an inhibitor of insulin actions." J Biol Chem. 1998; 273(40): 26026-35. PubMed: 9748281
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