| General information | | DisProt: | DP00500 | | Name: | DNA repair enzyme | | Synonym(s): | Q8MWS6_TOXGO
TgDRE
| | First appeared in release: | Release 3.2 (05/26/2006) | | UniProt: | Q8MWS6 | | UniGene: | | | SwissProt: | Q8MWS6_TOXGO | | TrEMBL: | | | NCBI (GI): | 21666569 | | Source organism: | Toxoplasma gondii | | Sequence length: | 466 | | Percent disordered: | 13% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MLDSLYGDLP PPSKDEGCPP KPTSLLGSLY DDLDDTPDTS SSSSASSSSS SSSALFASSS - 60
WTAERLQMLT PAVVRKQTLK SASGLGAASS QTAGQASSSK PPLDVAAVAA AASAAAAAVA - 120
AQLKAVKSEA FGDRARSLFG RGEREKAEAL GASDEAASAP GVAGGVKRDE SEKGESLRAL - 180
GRLGVETAEG RRGHQGGDKE TESSGFVSIG QMKDEYDPGT PNDYSAIYRE KIRKQQREEL - 240
ERLRQQELDK QRRDRELPGS GSSLSGVAFG DSPSTPSPPA APPAPPVLPS YIDPAKKSFA - 300
ARMMEKMGWK HGEGLGVNKQ GITAPLVAKK TAMSSGVIVQ GAEVVQAAAQ VWAQFNRPPT - 360
RVLLLLNMVG RGEVDEDLKE ETEEEAAKFG NLLQVKIVEA AEAPDDEAVR IFCEYERKEE - 420
ATRAFMTMNG RVFGGRTVKG RFYCEERWGK DDLMPNPEEE ADKPLD
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| Functional narrative |
Toxoplasma gondii is an obligate intracellular protozoan parasite infecting a broad range of warm-blooded vertebrates and up to 30% of the human population worldwide. T. gondii is recognized as an important opportunistic pathogen parasite associated with AIDS and congenital birth defects. T. gondii expresses the 55 kDa TgDRE protein which belongs to a novel family of proteins characterized by the presence of three domains, a human splicing factor 45-like motif (SF), a glycine-rich motif (G-patch), and a RNA recognition motif (RRM). The two latter domains are mainly known as RNA-binding domains, and their presence in TgDRE, whose partial DNA repair function was demonstrated, suggests that the protein could also be involved in the RNA metabolism. The SF45-like domain has a helical conformation and is involved in the oligomerization of the protein. The G-patch domain, mainly unstructured on its own as well as in the presence of the SF upstream and RRM downstream domains, is able to bind small RNA oligonucleotides.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | G-patch domain | | Location: | 284 - 343 | | Length: | 60 | | Region sequence: |
APPVLPSYIDPAKKSFAARMMEKMGWKHGEGLGVNKQGITAPLVAKKTAMSSGVIVQGAE
| | Modification type: | Fragment
Native
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-mRNA binding
Protein-DNA binding
Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7.4; 0.02 cm path length; 0.5 mg/mL protein; 20 mM phosphate)
- Nuclear magnetic resonance (NMR) (298 K; pH: 6.2; 1H, TOCSY, NOESY, and COSY; 1 mM protein; 20 mM sodium phosphate buffer with 0-150 mM NaCl)
| References:
- Frenal K, Callebaut I, Wecker K, Prochnicka-Chalufour A, Dendouga N, Zinn-Justin S, Delepierre M, Tomavo S, Wolff N. "Structural and Functional Characterization of the TgDRE Multidomain Protein, a DNA Repair Enzyme from Toxoplasma gondii." Biochemistry. 2006; 45(15): 4867-4874. PubMed: 16605254
| Comments:
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| References |
- Dendouga N, Callebaut I, Tomavo S. "A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii." Eur J Biochem. 2002; 269(14): 3393-401. PubMed: 12135477
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