| General information | | DisProt: | DP00594_A002 | | Name: | Probable nuclear hormone receptor HR38 [Isoform Short] | | Synonym(s): | HR38_DROME
dHR38
Nuclear receptor subfamily 4 group A member 4
P49869-2
| | First appeared in release: | Release 5.6 (01/10/2011) | | UniProt: | P49869 | | UniGene: | | | SwissProt: | HR38_DROME | | TrEMBL: | | | NCBI (GI): | | | Source organism: | Drosophila melanogaster (Fruit fly) | | Sequence length: | 551 | | Percent disordered: | 39% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MDEDCFPPLS GGWSASPPAP SQLQQLHTLQ SQAQMSHPNS SNNSSNNAGN SHNNSGGYNY - 60
HGHFNAINAS ANLSPSSSAS SLYEYNGVSA ADNFYGQQQQ QQQQSYQQHN YNSHNGERYS - 120
LPTFPTISEL AAATAAVEAA AAATVGGPPP VRRASLPVQR TVSPAGSTAQ SPKLAKITLN - 180
QRHSHAHAHA LQLNSAPNSA ASSPASADLQ AGRLLQAPSQ LCAVCGDTAA CQHYGVRTCE - 240
GCKGFFKRTV QKGSKYVCLA DKNCPVDKRR RNRCQFCRFQ KCLVVGMVKE VVRTDSLKGR - 300
RGRLPSKPKS PQESPPSPPI SLITALVRSH VDTTPDPSCL DYSHYEEQSM SEADKVQQFY - 360
QLLTSSVDVI KQFAEKIPGY FDLLPEDQEL LFQSASLELF VLRLAYRARI DDTKLIFCNG - 420
TVLHRTQCLR SFGEWLNDIM EFSRSLHNLE IDISAFACLC ALTLITERHG LREPKKVEQL - 480
QMKIIGSLRD HVTYNAEAQK KQHYFSRLLG KLPELRSLSV QGLQRIFYLK LEDLVPAPAL - 540
IENMFVTTLP F
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| Functional narrative |
Binds to NGFI-B response elements. Plays an important role in late stages of epidermal metamorphosis. Ubiquitously expressed in preblastoderm embryos, specifically in central nervous system and intestinal tract. Highly expressed in third instar larval imaginal disks and brain complexes, but not in ovaries. (UniProt)
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Pre-Molten Globule | | Name: | N-terminal domain | | Location: | 1 - 216 | | Length: | 216 | | Region sequence: |
MDEDCFPPLSGGWSASPPAPSQLQQLHTLQSQAQMSHPNSSNNSSNNAGNSHNNSGGYNY
HGHFNAINASANLSPSSSASSLYEYNGVSAADNFYGQQQQQQQQSYQQHNYNSHNGERYS
LPTFPTISELAAATAAVEAAAAATVSSPSVGGPPPVRRASLPVQRTVSPAGSTAQSPKLA
KITLNQRHSHAHAHALQLNSAPNSAASSPASADLQA | | Modification type: | Engineered
Fragment
| | PDB: | | | Structural/functional type: | Function arises via a pre-molten globule to order transition
| | Functional classes: | Modification site
Molecular recognition effectors
| | Functional subclasses: | Phosphorylation
Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (296 K; pH: 7; dHR38-NTD 4.5 uM; Na2HPO4 x 2H20 (buffer) 50 mM; NaCl (buffer) 150 mM; glycerol (buffer (v/v)) 10 %; DTT (buffer) 1 mM; GdmCl, TMAO or TFE (in absence or presence of); temperature range (293K - 353K))
- Fluorescence, intrinsic (296 K; pH: 7; dHR38-NTD 4.5 uM; DTT (buffer) 1 mM; GdmCl or TFE (in absence or presence of); glycerol (buffer (v/v)) 10 %; Na2HPO4 x 2H20 (buffer) 50 mM; NaCl (buffer) 150 mM)
- Size exclusion/gel filtration chromatography (298 K; pH: 7; dHR38-NTD 0.05 mg/mL; DTT (buffer) 1 mM; GdmCl (in absence or presence of, at appropriate concentration); glycerol (buffer (v/v)) 10 %; Na2HPO4 x 2H20 (buffer) 50 mM; NaCl (buffer) 150 mM)
- SDS-PAGE gel, Aberrant mobility on
- Sensitivity to proteolysis
- Radio-labelled Enzyme Activity Assay (303 K; pH: 7; ATP (2 uCi of [gamma-32P]ATP) 100 uM; dHR38-NTD 7 ug; DTT 1 mM; KCl 100 mM; MgCl2 10 mM; PKA or GSK-3beta (6.2 U or 8 U, respectively); Tris-HCl 25 mM)
- Radio-labelled Enzyme Activity Assay (310 K; pH: 7; ATP (2 uCi of [gamma-32P]ATP) 50 uM; CK2 33 uU; dHR38-NTD 7 ug; DTT 1 mM; MgCl2 10 mM; Tris-HCl 20 mM)
| References:
- Dziedzic-Letka A, Rymarczyk G, Kapłon TM, Górecki A, Szamborska-Gbur A, Wojtas M, Dobryszycki P, Ożyhar A. "Intrinsic disorder of Drosophila melanogaster hormone receptor 38 N-terminal domain." 2010. PubMed: 21064127
| Comments:The fragment of dHR38-NTD used by Dziedzic-Letka et al (2010) varies from the UniProt canonical sequence; the experimental fragment contains an insertion of 5 additional amino acids at aa 146-150 (SSPSV).
Dziedzic-Letka et al (2010) found that dHR38 is involved in "an unusual ecdysteroid signaling pathway that is strictly dHR38 dependent [but] does not rely on direct binding of ecdysteroids to dHR38".
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| References |
- Dziedzic-Letka A, Rymarczyk G, Kapłon TM, Górecki A, Szamborska-Gbur A, Wojtas M, Dobryszycki P, Ożyhar A. "Intrinsic disorder of Drosophila melanogaster hormone receptor 38 N-terminal domain." 2010. PubMed: 21064127
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| Comments |
Sent for AV 1-12-2011 (PubMed: 21064127)
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