DP00600: 50S ribosomal protein L4FASTA viewXML view

General information
DisProt:DP00600
Name:50S ribosomal protein L4
Synonym(s):RL4_ECOLI
First appeared in release:Release 5.5 (11/17/2010)
UniProt:P60723
UniGene: 
SwissProt: RL4_ECOLI
TrEMBL:  
NCBI (GI): 46397662
Source organism:Escherichia coli (strain K12)
Sequence length:201
Percent disordered:31%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW - 60
RQKGTGRARS GSIKSPIWRS GGVTFAARPQ DHSQKVNKKM YRGALKSILS ELVRQDRLIV - 120
VEKFSVEAPK TKLLAQKLKD MALEDVLIIT GELDENLFLA ARNLHKVDVR DATGIDPVSL - 180
IAFDKVVMTA DAVKQVEEML A



Functional narrative    

One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. The inner loop of L13 is fully disordered. Protein L4 is a both a transcriptional repressor and a translational repressor protein; these two functions are independent of each other. It regulates transcription of the S10 operon (to which L4 belongs) by causing premature termination of transcription within the S10 leader; termination absolutely requires the nusA protein. L4 controls the translation of the S10 operon by binding to its mRNA. The regions of L4 that control regulation (residues 131-210) are different from those required for ribosome assembly (residues 89-103). Forms part of the polypeptide exit tunnel. Can regulate expression from Citrobacter freundii, Haemophilus influenzae, Morganella morganii, Salmonella typhimurium, Serratia marcescens, Vibrio cholerae and Yersinia enterocolitica (but not Pseudomonas aeruginosa) S10 leaders in vitro.

Region 2: 41-96 Region 1: 45-103

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:inner loop
Location:45 - 103
Length:59
Region sequence:

AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRG

Modification type: Native
PDB:  
Structural/functional type: Function arises from the disordered state
Functional classes: Molecular recognition effectors
Functional subclasses: Protein-rRNA binding
Detection methods:
 
References:
  1. Timsit Y, Acosta Z, Allemand F, Chiaruttini C, Springer M. "The role of disordered ribosomal protein extensions in the early steps of eubacterial 50 s ribosomal subunit assembly." Int J Mol Sci. 2009; 10(3): 817-34. PubMed: 19399222

Comments:
 



Region 2
Type:Disordered
Name:Inner Loop
Location:41 - 96
Length:56
Region sequence:

QGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKV

Modification type: Native
PDB: 1DMG:A
Structural/functional type: Function arises from the disordered state
Functional classes: Molecular recognition effectors
Functional subclasses: Protein-rRNA binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (HEPES (buffer) 10 mM; Jasco J-715 (spectropolarimeter ); protein (sample) 0.1086 μg/ml)

References:
  1. Timsit, Youri, Fr├ęderic Allemand, Claude Chiaruttini, and Mathias Springer. "Coexistence of two protein folding states in the crystal structure of ribosomal protein L20." Embo J. 2006; 7(10): 1013-1018. PubMed: 1618378

Comments:
 


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