Annotations for this protein have been verified by the authors of the corresponding papers


DP00610: Translocase of chloroplast 132, chloroplasticFASTA viewXML view

General information
DisProt:DP00610
Name:Translocase of chloroplast 132, chloroplastic
Synonym(s):TC132_ARATH
AtToc132
132 kDa chloroplast outer envelope protein
Translocon at the outer envelope membrane of chloroplasts 132
First appeared in release:Release 5.1 (05/28/2010)
UniProt:Q9SLF3
UniGene:At.21953
SwissProt: TC132_ARATH
TrEMBL:  
NCBI (GI): 75206664
Source organism:Arabidopsis thaliana (Mouse-ear cress)
Sequence length:1206
Percent disordered:38%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MGDGTEFVVR SDREDKKLAE DRISDEQVVK NELVRSDEVR DDNEDEVFEE AIGSENDEQE - 60
EEEDPKRELF ESDDLPLVET LKSSMVEHEV EDFEEAVGDL DETSSNEGGV KDFTAVGESH - 120
GAGEAEFDVL ATKMNGDKGE GGGGGSYDKV ESSLDVVDTT ENATSTNTNG SNLAAEHVGI - 180
ENGKTHSFLG NGIASPKNKE VVAEVIPKDD GIEEPWNDGI EVDNWEERVD GIQTEQEVEE - 240
GEGTTENQFE KRTEEEVVEG EGTSKNLFEK QTEQDVVEGE GTSKDLFENG SVCMDSESEA - 300
ERNGETGAAY TSNIVTNASG DNEVSSAVTS SPLEESSSGE KGETEGDSTC LKPEQHLASS - 360
PHSYPESTEV HSNSGSPGVT SREHKPVQSA NGGHDVQSPQ PNKELEKQQS SRVHVDPEIT - 420
ENSHVETEPE VVSSVSPTES RSNPAALPPA RPAGLGRASP LLEPASRAPQ QSRVNGNGSH - 480
NQFQQAEDST TTEADEHDET REKLQLIRVK FLRLAHRLGQ TPHNVVVAQV LYRLGLAEQL - 540
RGRNGSRVGA FSFDRASAMA EQLEAAGQDP LDFSCTIMVL GKSGVGKSAT INSIFDEVKF - 600
CTDAFQMGTK RVQDVEGLVQ GIKVRVIDTP GLLPSWSDQA KNEKILNSVK AFIKKNPPDI - 660
VLYLDRLDMQ SRDSGDMPLL RTISDVFGPS IWFNAIVGLT HAASVPPDGP NGTASSYDMF - 720
VTQRSHVIQQ AIRQAAGDMR LMNPVSLVEN HSACRTNRAG QRVLPNGQVW KPHLLLLSFA - 780
SKILAEANAL LKLQDNIPGR PFAARSKAPP LPFLLSSLLQ SRPQPKLPEQ QYGDEEDEDD - 840
LEESSDSDEE SEYDQLPPFK SLTKAQMATL SKSQKKQYLD EMEYREKLLM KKQMKEERKR - 900
RKMFKKFAAE IKDLPDGYSE NVEEESGGPA SVPVPMPDLS LPASFDSDNP THRYRYLDSS - 960
NQWLVRPVLE THGWDHDIGY EGVNAERLFV VKEKIPISVS GQVTKDKKDA NVQLEMASSV - 1020
KHGEGKSTSL GFDMQTVGKE LAYTLRSETR FNNFRRNKAA AGLSVTHLGD SVSAGLKVED - 1080
KFIASKWFRI VMSGGAMTSR GDFAYGGTLE AQLRDKDYPL GRFLTTLGLS VMDWHGDLAI - 1140
GGNIQSQVPI GRSSNLIARA NLNNRGAGQV SVRVNSSEQL QLAMVAIVPL FKKLLSYYYP - 1200
QTQYGQ



Functional narrative    

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast.

Region 1: 1-455

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:N-terminal acidic (A-) domain
Location:1 - 455
Length:455
Region sequence:

MGDGTEFVVRSDREDKKLAEDRISDEQVVKNELVRSDEVRDDNEDEVFEEAIGSENDEQE
EEEDPKRELFESDDLPLVETLKSSMVEHEVEDFEEAVGDLDETSSNEGGVKDFTAVGESH
GAGEAEFDVLATKMNGDKGEGGGGGSYDKVESSLDVVDTTENATSTNTNGSNLAAEHVGI
ENGKTHSFLGNGIASPKNKEVVAEVIPKDDGIEEPWNDGIEVDNWEERVDGIQTEQEVEE
GEGTTENQFEKRTEEEVVEGEGTSKNLFEKQTEQDVVEGEGTSKDLFENGSVCMDSESEA
ERNGETGAAYTSNIVTNASGDNEVSSAVTSSPLEESSSGEKGETEGDSTCLKPEQHLASS
PHSYPESTEVHSNSGSPGVTSREHKPVQSANGGHDVQSPQPNKELEKQQSSRVHVDPEIT
ENSHVETEPEVVSSVSPTESRSNPAALPPARPAGL

Modification type: Native
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular recognition effectors
Functional subclasses: Substrate/ligand binding
Protein-protein binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 8; )
References:
  1. Richardson LG, Jelokhani-Niaraki M, Smith MD. "The acidic domains of the Toc159 chloroplast preprotein receptor family are intrinsically disordered protein domains." BMC Biochem. 2009; 10(1): 35. PubMed: 20042108
Comments:
The A-domain was shown experimentally to be disordered under non-denaturing conditions, and underwent structural changes characteristic of IDPs at extremes of temperature and pH. Furthermore, in the presence of 50% TFE, the A-domain gained considerable structure.


References

  1. Richardson LG, Jelokhani-Niaraki M, Smith MD. "The acidic domains of the Toc159 chloroplast preprotein receptor family are intrinsically disordered protein domains." BMC Biochem. 2009; 10(1): 35. PubMed: 20042108



Comments


[PMID 20042108 ]


Additional UniGene ID: At.67099


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