| General information | | DisProt: | DP00646 | | Name: | Gap junction alpha-5 protein | | Synonym(s): | CXA5_MOUSE
Connexin-40
Cx40
| | First appeared in release: | Release 5.1 (05/28/2010) | | UniProt: | Q01231 | | UniGene: | Mm.281816 | | SwissProt: | CXA5_MOUSE | | TrEMBL: | | | NCBI (GI): | 231921 | | Source organism: | Mus musculus (Mouse) | | Sequence length: | 358 | | Percent disordered: | 29% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA DFRCDTIQPG - 60
CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV RMQEKQKLRD AEKAKEAHRT - 120
GAYEYPVAEK AELSCWKEVD GKIVLQGTLL NTYVCTILIR TTMEVAFIVG QYLLYGIFLD - 180
TLHVCRRSPC PHPVNCYVSR PTEKNVFIVF MMAVAGLSLF LSLAELYHLG WKKIRQRFGK - 240
SRQGVDKHQL PGPPTSLVQS LTPPPDFNQC LKNSSGEKFF SDFSNNMGSR KNPDALATGE - 300
VPNQEQIPGE GFIHMHYSQK PEYASGASAG HRLPQGYHSD KRRLSKASSK ARSDDLSV
|
| Functional narrative |
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
|
| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
|
| Region 1 | | Type: | Disordered | | Name: | C-terminal | | Location: | 251 - 355 | | Length: | 105 | | Region sequence: |
PGPPTSLVQSLTPPPDFNQCLKNSSGEKFFSDFSNNMGSRKNPDALATGEVPNQEQIPGE
GFIHMHYSQKPEYASGASAGHRLPQGYHSDKRRLSKASSKARSDD | | Modification type: | Native
| | PDB: | 2K7M:A | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (280 K; )
- Fourier transform infrared spectroscopy (FTIR), aka infrared spectroscopy) (293 K; pH: 6; CaF2 100 uL; Cx40CT (sample) 60 uL; PBS (buffer) 0.7 mM; resolution 2 cm-1)
- Circular dichroism (CD) spectroscopy, near-UV (280 K; bandwidth 4 nm; integration time 1 s; path length 1 mm; protein (sample) 200 uM; scan rate 1.6 nm/s)
- Analytical ultracentrifugation (293 K; pH: 6; dithiothreitol 1 mm; PH (6.0 and 7.4); three concentrations (A280 = 0.2, 0.4, and 0.8); three rotor speeds (24,000, 25,000, and 26,000 ))
| References:
- Bouvier D, Spagnol G, Chenavas S, Kieken F, Vitrac H, Brownell S, Kellezi A, Forge V, Sorgen PL. "Characterization of the structure and intermolecular interactions between the connexin40 and connexin43 carboxyl-terminal and cytoplasmic loop domains." J Biol Chem. 2009; 284(49): 34257-71. PubMed: 19808665
| Comments:
|
| Comments |
Additional UniGene ID: Mm.444963
AV (6-4-2010)
PubMed: 19808665
|
| If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us. |
Disprot-footer
|
