DP00651_C007: Integrase p46FASTA viewXML view

General information
DisProt:DP00651_C007
Name:Integrase p46
Synonym(s):POL_MLVMO
[cleavage product of Gag-Pol polyprotein]
Integrase p46 [cleavage product 7]
IN
First appeared in release:Release 5.1 (05/28/2010)
UniProt:P03355
UniGene: 
SwissProt: POL_MLVMO
TrEMBL:  
NCBI (GI): 284018164
Source organism:Moloney murine leukemia virus (MoMLV)
Sequence length:408
Percent disordered:3%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
IENSSPYTSE HFHYTVTDIK DLTKLGAIYD KTKKYWVYQG KPVMPDQFTF ELLDFLHQLT - 60
HLSFSKMKAL LERSHSPYYM LNRDRTLKNI TETCKACAQV NASKSAVKQG TRVRGHRPGT - 120
HWEIDFTEIK PGLYGYKYLL VFIDTFSGWI EAFPTKKETA KVVTKKLLEE IFPRFGMPQV - 180
LGTDNGPAFV SKVSQTVADL LGIDWKLHCA YRPQSSGQVE RMNRTIKETL TKLTLATGSR - 240
DWVLLLPLAL YRARNTPGPH GLTPYEILYG APPPLVNFPD PDMTRVTNSP SLQAHLQALY - 300
LVQHEVWRPL AAAYQEQLDR PVVPHPYRVG DTVWVRRHQT KNLEPRWKGP YTVLLTTPTA - 360
LKVDGIAAWI HAAHVKAADP GGGPSSRLTW RVQRSQNPLK IRLTREAP



Functional narrative    

Integrase catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. This enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA. The first step in the integration process is 3' processing. This step requires a complex comprising the viral genome, matrix protein, Vpr and integrase. This complex is called the pre-integration complex (PIC). The integrase protein removes 2 nucleotides from each 3' end of the viral DNA, leaving recessed CA OH's at the 3' ends. In the second step, the PIC enters cell nucleus. This process is mediated through integrase and Vpr proteins, and allow the virus to infect a non dividing cell. This ability to enter the nucleus is specific of lentiviruses, other retroviruses cannot and rely on cell division to access cell chromosomes. In the third step, termed strand transfer, the integrase protein joins the previously processed 3' ends to the 5' ends of strands of target cellular DNA at the site of integration. The 5'-ends are produced by integrase-catalyzed staggered cuts, 5 bp apart. A Y-shaped, gapped, recombination intermediate results, with the 5'-ends of the viral DNA strands and the 3' ends of target DNA strands remaining unjoined, flanking a gap of 5 bp. The last step is viral DNA integration into host chromosome. This involves host DNA repair synthesis in which the 5 bp gaps between the unjoined strands are filled in and then ligated. Since this process occurs at both cuts flanking the HIV genome, a 5 bp duplication of host DNA is produced at the ends of HIV-1 integration. Alternatively, Integrase may catalyze the excision of viral DNA just after strand transfer, this is termed disintegration.

Region 1: 207-218

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:207 - 218
Length:12
Region sequence:

LHCAYRPQSSGQ

Modification type: Engineered
Fragment
PDB:  
Structural/functional type: Function arises from the disordered state
Functional classes: Molecular assembly
Entropic chain
Functional subclasses: Flexible linkers/spacers
Protein-DNA binding
Protein-protein binding
Detection methods:
  1. Radio-labelled Enzyme Activity Assay

  2. High relative B-factor

References:
  1. Acevedo ML, Arbildúa JJ, Monasterio O, Toledo H, León O. "Role of the 207-218 peptide region of Moloney murine leukemia virus integrase in enzyme catalysis." Arch Biochem Biophys. 2009. PubMed: 20026028

Comments:
 


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