| General information | | DisProt: | DP00652 | | Name: | Superoxide dismutase [Cu-Zn] | | Synonym(s): | SODC_HUMAN
EC=1.15.1.1
| | First appeared in release: | Release 5.2 (08/07/2010) | | UniProt: | P00441 | | UniGene: | Hs.443914 | | SwissProt: | SODC_HUMAN | | TrEMBL: | | | NCBI (GI): | 134611 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 154 | | Percent disordered: | 19% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS - 60
AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV - 120
HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
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| Functional narrative |
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | Subunit A Metal-free A4V mutant | | Location: | 67 - 79 | | Length: | 13 | | Region sequence: |
PLSRKHGGPKDEE | | Modification type: | Natural variant
| | PDB: | 3GZQ:A, 3GZQ:B | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Metal binding
| Detection methods:
- X-ray crystallography (298 K; pH: 6; Area Detector Systems Corporation Quantum 315 (CCD detector); hanging drop vapor diffusion method (sample); protein 17 mg/mL; sodium acetate 10 mM; sodium chloride 125 mM; ammonium sulfate 1.6 M; MES 0.1 M; TCEP 5 mM)
- Analytical ultracentrifugation (293 K; pH: 5.5; Beckman Optima XL-I (centrifuge); protein 2.4 mg/mL; sodium acetate; speed 50000 ; TCEP)
| References:
- Galaleldeen A, Strange RW, Whitson LJ, Antonyuk SV, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, Hart PJ. "Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A." Arch Biochem Biophys. 2009. PubMed: 19800308
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | Subunit A Metal-free A4V mutant | | Location: | 126 - 141 | | Length: | 16 | | Region sequence: |
DLGKGGNEESTKTGNA | | Modification type: | Natural variant
| | PDB: | 3GZQ:A, 3GZQ:B | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Metal binding
| Detection methods:
- X-ray crystallography (298 K; pH: 6; Area Detector Systems Corporation Quantum 315 (CCD detector); hanging drop vapor diffusion method; protein (sample) 17 mg/mL; sodium acetate 10 mM; sodium chloride 125 mM; ammonium sulfate 1.6 M; MES 0.1 M; TCEP 5 mM)
- Analytical ultracentrifugation (293 K; pH: 5.5; Beckman Optima XL-I (centrifuge); protein (sample) 2.4 mg/mL; sodium acetate; speed 50000 rpm; TCEP)
| References:
- Galaleldeen A, Strange RW, Whitson LJ, Antonyuk SV, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, Hart PJ. "Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A." Arch Biochem Biophys. 2009. PubMed: 19800308
| Comments:
|
| Region 3 | | Type: | Disordered | | Name: | Subunit B Metal-free A4V mutant | | Location: | 68 - 79 | | Length: | 12 | | Region sequence: |
LSRKHGGPKDEE | | Modification type: | Natural variant
| | PDB: | 3GZQ:A, 3GZQ:B | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Metal binding
| Detection methods:
- X-ray crystallography (298 K; pH: 6; Area Detector Systems Corporation Quantum 315 (CCD detector); hanging drop vapor diffusion method; protein (sample) 17 mg/mL; sodium acetate 10 mM; sodium chloride 125 mM; ammonium sulfate 1.6 M; MES 0.1 M; TCEP 5 mM)
- Analytical ultracentrifugation (293 K; pH: 5.5; Beckman Optima XL-I (centrifuge); protein (sample) 2.4 mg/mL; sodium acetate; speed 50000 ; TCEP)
| References:
- Galaleldeen A, Strange RW, Whitson LJ, Antonyuk SV, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, Hart PJ. "Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A." Arch Biochem Biophys. 2009. PubMed: 19800308
| Comments:
|
| Region 4 | | Type: | Disordered | | Name: | Subunit B Metal-free A4V mutant | | Location: | 125 - 141 | | Length: | 17 | | Region sequence: |
DDLGKGGNEESTKTGNA | | Modification type: | Natural variant
| | PDB: | 3GZQ:A, 3GZQ:B | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Metal binding
| Detection methods:
- X-ray crystallography (298 K; pH: 6; ammonium sulfate 1.6 M; Area Detector Systems Corporation Quantum 315 (CCD detector); hanging drop vapor diffusion method; MES 0.1 M; protein (sample) 17 mg/mL; sodium acetate 10 mM; sodium chloride 125 mM; TCEP 5 mM)
- Analytical ultracentrifugation (293 K; pH: 5.5; Beckman Optima XL-I (centrifuge); protein (sample) 2.4 mg/mL; sodium acetate; speed 50000 rpm; TCEP)
| References:
- Galaleldeen A, Strange RW, Whitson LJ, Antonyuk SV, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, Hart PJ. "Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A." Arch Biochem Biophys. 2009. PubMed: 19800308
| Comments:
|
| Region 5 | | Type: | Disordered | | Name: | Subunit C/D Metal-free G93A mutant | | Location: | 67 - 78 | | Length: | 12 | | Region sequence: |
PLSRKHGGPKDE | | Modification type: | Natural variant
| | PDB: | 3GZP:A, 3GZP:B, 3GZP:C, 3GZP:D | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Metal binding
| Detection methods:
- X-ray crystallography (298 K; pH: 5.5; ammonium sulfate 2.4 M; CCD detector; hanging drop vapor diffusion method; HEPES 0.1 M; protein 5 mg/mL; sodium acetate 10 mM; sodium chloride 125 mM)
- Analytical ultracentrifugation (293 K; pH: 5.5; Beckman Optima XL-I (centrifuge); protein (sample) 2.4 mg/mL; sodium acetate; speed 50000 rpm; TCEP)
| References:
- Galaleldeen A, Strange RW, Whitson LJ, Antonyuk SV, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, Hart PJ. "Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A." Arch Biochem Biophys. 2009. PubMed: 19800308
| Comments:
|
| Region 6 | | Type: | Disordered | | Name: | Subunit C/D Metal-free G93A mutant | | Location: | 125 - 139 | | Length: | 15 | | Region sequence: |
DDLGKGGNEESTKTG | | Modification type: | Natural variant
| | PDB: | 3GZP:A, 3GZP:B, 3GZP:C, 3GZP:D | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Metal binding
| Detection methods:
- X-ray crystallography (298 K; pH: 5.5; ammonium sulfate 2.4 M; CCD detector; hanging drop vapor diffusion method; HEPES 0.1 M; protein (sample) 5 mg/mL; sodium acetate 10 mM; sodium chloride 125 mM)
- Analytical ultracentrifugation (293 K; pH: 5.5; Beckman Optima XL-I (centrifuge); protein (sample) 2.4 mg/mL; sodium acetate; speed 50000 rpm; TCEP)
| References:
- Galaleldeen A, Strange RW, Whitson LJ, Antonyuk SV, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, Hart PJ. "Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A." Arch Biochem Biophys. 2009. PubMed: 19800308
| Comments:
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| References |
- Galaleldeen A, Strange RW, Whitson LJ, Antonyuk SV, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, Hart PJ. "Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A." Arch Biochem Biophys. 2009. PubMed: 19800308
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| Comments |
AV (8-2-2010) PubMed: 19800308
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