| General information | | DisProt: | DP00656 | | Name: | Transcriptional repressor protein korB | | Synonym(s): | KORB2_ECOLX
korB
| | First appeared in release: | Release 5.3 (09/21/2010) | | UniProt: | P07674 | | UniGene: | | | SwissProt: | KORB2_ECOLX | | TrEMBL: | | | NCBI (GI): | | | Source organism: | | | Sequence length: | 358 | | Percent disordered: | 30% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MTAAQAKTTK KNTAAAAQEA AGAAQPSGLG LDSIGDLSSL LDAPAASQGG SGPIELDLDL - 60
IDEDPHQPRT ADNPGFSPES IAEIGATIKE RGVKSPISVR ENQEQPGRYI INHGARRYRG - 120
SKWAGKKSIP AFIDNDYNEA DQVIENLQRN ELTPREIADF IGRELAKGKK KGDIAKEIGK - 180
SPAFITQHVT LLDLPEKIAD AFNTGRVRDV TVVNELVTAF KKRPEEVEAW LDDDTQEITR - 240
GTVKLLREFL DEKGRDPNTV DAFNGQTDAE RDAEAGDGQD GEDGDQDGKD AKEKGAKEPD - 300
PDKLKKAIVQ VEHDERPARL ILNRRPPAEG YAWLKYEDDG QEFEANLADV KLVALIEG
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| Functional narrative |
In conjunction with korA, inhibits the transcription of the kilA, trfA and korAB operons. Is also involved in the negative control of the kilB operon.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | N-terminal domain | | Location: | 1 - 64 | | Length: | 64 | | Region sequence: |
MTAAQAKTTKKNTAAAAQEAAGAAQPSGLGLDSIGDLSSLLDAPAASQGGSGPIELDLDL
IDED | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Modification site
Molecular recognition effectors
| | Functional subclasses: | Protein-DNA binding
Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; KorB (protein) 0.02 mg/mL; sodium phosphate (buffer) 10 mM; NaClO4 20 mM)
- Nuclear magnetic resonance (NMR) (298 K; pH: 7; EDTA 0.1 mM; KorB (protein) 0.5 mM; NaCl 100 mM; sodium phosphate (buffer) 10 mM)
- Analytical ultracentrifugation (293 K; pH: 7; EDTA 0.1 mM; KorB protein (Concentration: 0.1-1 mg/mL); NaCl 100 mM; Tris-HCL 10 mM)
- Size exclusion/gel filtration chromatography (293 K; pH: 7; EDTA 0.1 mM; KorB protein (Concentration: 5-10 mg/mL); NaCl 100 mM; phenylmethylsulfonyl fluoride (0.8 mL/minute) 10 mg/L; Tris-HCL)
- Small-angle X-ray scattering (SAXS) (293 K; pH: 7; EDTA 0.1 mM; KorB protein (Concentration: 3-15 mg/mL); NaCl 100 mM; Tris-HCl 10 mM)
| References:
- Rajasekar K, Muntaha ST, Tame JR, Kommareddy S, Morris G, Wharton CW, Thomas CM, White SA, Hyde EI, Scott DJ. "Order and disorder in the domain organization of the plasmid partition protein KorB." J. Biol. Chem.. 2010; 285(20): 15440-9. PubMed: 20200158
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | linker region | | Location: | 252 - 294 | | Length: | 43 | | Region sequence: |
EKGRDPNTVDAFNGQTDAERDAEAGDGQDGEDGDQDGKDAKEK | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Molecular recognition effectors
| | Functional subclasses: | Flexible linkers/spacers
Protein-DNA binding
Protein inhibitor
Protein-protein binding
| Detection methods:
| References:
- Rajasekar K, Muntaha ST, Tame JR, Kommareddy S, Morris G, Wharton CW, Thomas CM, White SA, Hyde EI, Scott DJ. "Order and disorder in the domain organization of the plasmid partition protein KorB." J. Biol. Chem.. 2010; 285(20): 15440-9. PubMed: 20200158
| Comments:
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| References |
- Rajasekar K, Muntaha ST, Tame JR, Kommareddy S, Morris G, Wharton CW, Thomas CM, White SA, Hyde EI, Scott DJ. "Order and disorder in the domain organization of the plasmid partition protein KorB." J. Biol. Chem.. 2010; 285(20): 15440-9. PubMed: 20200158
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| Comments |
Sent for AV 9-15-2010 (PMID 20200158).
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