| General information | | DisProt: | DP00665 | | Name: | Seed maturation protein PM28 | | Synonym(s): | Q9XES8_SOYBN
GmPM28
| | First appeared in release: | Release 5.1 (05/28/2010) | | UniProt: | Q9XES8 | | UniGene: | Gma.168 | | SwissProt: | Q9XES8_SOYBN | | TrEMBL: | | | NCBI (GI): | 75215230 | | Source organism: | Glycine max (Soybean) | | Sequence length: | 89 | | Percent disordered: | 100% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAKSKEDITY ATSQARLSED EAVRVAYEHG SPLEGGKIAD SQPVDLFSSA HNMPKSGQTT - 60
MDSNTSDQSQ MQRDTQEGGS KEFTTGAPG
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| Functional narrative |
Seed maturation protein PM28 is one of the Late Embryogenesis-abundant Group IV (LEA IV) proteins. Function and mechanisms are not currently known, but they may play a protective role for membranes and proteins sensitive to dehydration. LEA IV proteins are also an important component of cellular glasses.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Molten Globule | | Name: | | | Location: | 1 - 89 | | Length: | 89 | | Region sequence: |
MAKSKEDITYATSQARLSEDEAVRVAYEHGSPLEGGKIADSQPVDLFSSAHNMPKSGQTT
MDSNTSDQSQMQRDTQEGGSKEFTTGAPG | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a molten globule to order transition
| | Functional classes: | Chaperones
| | Functional subclasses: | Unknown
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (283 K; pH: 7; Potassium phosphate (buffer) 10 mM; Recombinant protein soln 10 uM; SDS (4%); Temp range 283K to 363K; TFE (50%))
- Fourier transform infrared spectroscopy (FTIR), aka infrared spectroscopy) (Aqueous lyophilized protein in D2O; Dry protein/sugar/poly-L-lysine/phospholipid (films for air-drying, 8 uL droplet of 20 ug/uL in H2O); Fast air-drying (RT, 3% RH, 3h); Slow air-drying (RT, 65% RH 16h + 3% RH, 3h); Temp range 233K to 423K)
| References:
- Shih MD, Hsieh TY, Lin TP, Hsing YI, Hoekstra FA. "Characterization of two soybean (Glycine max L.) LEA IV proteins by circular dichroism and Fourier transform infrared spectrometry." Plant Cell Physiol. . PubMed: 20071374
| Comments:
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| References |
- Shih MD, Hsieh TY, Lin TP, Hsing YI, Hoekstra FA. "Characterization of two soybean (Glycine max L.) LEA IV proteins by circular dichroism and Fourier transform infrared spectrometry." Plant Cell Physiol. . PubMed: 20071374
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