| General information | | DisProt: | DP00683 | | Name: | Homeobox protein Nkx-3.1 | | Synonym(s): | NKX31_HUMAN
Homeobox protein NK-3 homolog A
| | First appeared in release: | Release 5.1 (05/28/2010) | | UniProt: | Q99801 | | UniGene: | Hs.55999 | | SwissProt: | NKX31_HUMAN | | TrEMBL: | | | NCBI (GI): | 3023954 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 234 | | Percent disordered: | 79% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MLRVPEPRPG EAKAEGAAPP TPSKPLTSFL IQDILRDGAQ RQGGRTSSQR QRDPEPEPEP - 60
EPEGGRSRAG AQNDQLSTGP RAAPEEAETL AETEPERHLG SYLLDSENTS GALPRLPQTP - 120
KQPQKRSRAA FSHTQVIELE RKFSHQKYLS APERAHLAKN LKLTETQVKI WFQNRRYKTK - 180
RKQLSSELGD LEKHSSLPAL KEEAFSRASL VSVYNSYPYY PYLYCVGSWS PAFW
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| Functional narrative |
Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Could play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate.
Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF. Interacts with WDR77. P11387:TOP1; NbExp=3; IntAct=EBI-1385894, EBI-876302;
Highly expressed in the prostate and, at a lower level, in the testis.
NKX3-1 has been thought to be one of the target gene of the 8p21 loss of heterozygosity, common in prostate cancer, but neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.
Belongs to the NK-3 homeobox family.
Contains 1 homeobox DNA-binding domain.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | N-terminal domain | | Location: | 1 - 184 | | Length: | 184 | | Region sequence: |
MLRVPEPRPGEAKAEGAAPPTPSKPLTSFLIQDILRDGAQRQGGRTSSQRQRDPEPEPEP
EPEGGRSRAGAQNDQLSTGPRAAPEEAETLAETEPERHLGSYLLDSENTSGALPRLPQTP
KQPQKRSRAAFSHTQVIELERKFSHQKYLSAPERAHLAKNLKLTETQVKIWFQNRRYKTK
RKQL | | Modification type: | Native
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Intraprotein interaction
Protein-DNA binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (pH: 6.5; Jasco J-715 (spectropolarimeter); MES (buffer) 20 mM; NaCl (buffer) 50 mM; path length (cuvette) 1 mm; termperature scanning 1 C/min)
- Nuclear magnetic resonance (NMR) (285 K; pH: 6.5; 5% D2O; MES (buffer) 20 mM; NaCl (buffer) 50 mM)
| References:
- Ju JH, Maeng JS, Lee DY, Piszczek G, Gelmann EP, Gruschus JM. "Interactions of the acidic domain and SRF interacting motifs with the NKX3.1 homeodomain." Biochem J. 2009; 48(44): 10601-10607. PubMed: 19780584
| Comments:
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| References |
- Ju JH, Maeng JS, Lee DY, Piszczek G, Gelmann EP, Gruschus JM. "Interactions of the acidic domain and SRF interacting motifs with the NKX3.1 homeodomain." Biochem J. 2009; 48(44): 10601-10607. PubMed: 19780584
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