DP00743: Cold-regulated protein 15bFASTA viewXML view

General information
DisProt:DP00743
Name:Cold-regulated protein 15b
Synonym(s):Q9SIN5_ARATH
Cold-regulated protein cor15
Cold-regulated protein cor15b
Putative cold-regulated protein cor15b
COR15B
First appeared in release:Release 6.02 (05/24/2013)
UniProt:Q9SIN5
UniGene:At.299
SwissProt: Q9SIN5_ARATH
TrEMBL:  
NCBI (GI):  
Source organism:Arabidopsis thaliana (Mouse-ear cress)
Sequence length:141
Percent disordered:64%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MAMSLSGAVL SGMGSSFHNV GAKQSGVGTV RVGRKSELVV VAQRKKSLIY AVKSDGNILD - 60
DLNEATKKAS DFVTDKTKEA LADGEKTKDY IVEKTIEANE TATEEAKKAL DYVTEKGKEA - 120
GNKAAEFVEG KAEEAKNATK S



Functional narrative    

COR15b is a cold-regulated gene of Arabidopsis thaliana that encodes a chloroplast-targeted polypeptide.

A putative signal peptide is found at aa M1-A51.

Region 1: 52-141

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:mature COR15B
Location:52 - 141
Length:90
Region sequence:

VKSDGNILDDLNEATKKASDFVTDKTKEALADGEKTKDYIVEKTIEANETATEEAKKALD
YVTEKGKEAGNKAAEFVEGKAEEAKNATKS

Modification type: Native
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes:  
Functional subclasses: Protein-lipid interaction
Protein-protein binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (or dried rCor15b 2 mg/mL; rCor15b (in H2O) 0.75 mg/mL)

  2. SDS-PAGE gel, Aberrant mobility on

References:
  1. Thalhammer A, Hundertmark M, Popova AV, Seckler R, Hincha DK. "Interaction of two intrinsically disordered plant stress proteins (COR15A and COR15B) with lipid membranes in the dry state." Biochim. Biophys. Acta. 2010; 1798(9): 1812-20. PubMed: 20510170

Comments:
Thalhammer et al (2010) found that both COR15A and COR15B are fully unstructured in solution, but gain alpha-helical structure upon drying. To locate helices, the authors ran predictions which found alpha-helices predicted at about 58-81 and 97-137. Both helices appear to have amphipathic nature.

Experiments were performed on mature COR15A and mature COR15B without the signal peptides.




Comments


See also DP00536:COR15A


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