Annotations for this protein have been verified by the authors of the corresponding papers



DP00014: ClusterinFASTA viewXML view

General information
DisProt:DP00014
Name:Clusterin
Synonym(s):CLUS_RAT
Sulfated glycoprotein 2
SGP-2
Dimeric acid glycoprotein
DAG
Testosterone repressed prostate message 2
TRPM-2
Clusterin beta chain (cleavage chain 1)
Clusterin alpha chain (cleavage chain 2)
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P05371
UniGene:Rn.1780
SwissProt: CLUS_RAT
TrEMBL:  
NCBI (GI): 461756
Source organism:Rattus norvegicus (Rat)
Sequence length:447
Percent disordered:26%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MKILLLCVAL LLTWDNGMVL GEQEFSDNEL QELSTQGSRY VNKEIQNAVQ GVKHIKTLIE - 60
KTNAERKSLL NSLEEAKKKK EGALDDTRDS EMKLKAFPEV CNETMMALWE ECKPCLKHTC - 120
MKFYARVCRS GSGLVGRQLE EFLNQSSPFY FWMNGDRIDS LLESDRQQSQ VLDAMQDSFT - 180
RASGIIDTLF QDRFFTHEPQ DIHHFSPMGF PHKRPHFLYP KSRLVRSLMP LSHYGPLSFH - 240
NMFQPFFDMI HQAQQAMDVQ LHSPALQFPD VDFLKEGEDD PTVCKEIRHN STGCLKMKGQ - 300
CEKCQEILSV DCSTNNPAQA NLRQELNDSL QVAERLTQQY NELLHSLQSK MLNTSSLLEQ - 360
LNDQFTWVSQ LANLTQGDDQ YLRVSTVTTH SSDSEVPSRV TEVVVKLFDS DPITVVLPEE - 420
VSKDNPKFMD TVAEKALQEY RRKSRME



Functional narrative    

Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death.

Region 1: 22-41 Region 2: 66-97 Region 3: 386-447

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:22 - 41
Length:20
Region sequence:

EQEFSDNELQELSTQGSRYV

Modification type:  
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Protein detergent
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; Clusterin protein (prepared in PBS) 4 uM)

  2. Nuclear magnetic resonance (NMR)

  3. Sensitivity to proteolysis (310 K; Clusterin (renatured or partially denatured); Deionized H2O (200 uL); Tris (pH 7.3, 10 uL) 1 M; Trypsin (4 ug))

References:
  1. Bailey RW, Dunker AK, Brown CJ, Garner EC, Griswold MD. "Clusterin, a binding protein with a molten globule-like region." Biochemistry. 2001; 40(39): 11828-40. PubMed: 11570883

Comments:
This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.




Region 2
Type:Disordered
Name: 
Location:66 - 97
Length:32
Region sequence:

RKSLLNSLEEAKKKKEGALDDTRDSEMKLKAF

Modification type:  
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Protein detergent
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; Clusterin protein (prepared in PBS) 4 uM)

  2. Nuclear magnetic resonance (NMR)

  3. Sensitivity to proteolysis (310 K; Clusterin (renatured or partially denatured); Deionized H2O (200 uL); Tris (pH 7.3, 10 uL) 1 M; Trypsin (4 ug))

References:
  1. Bailey RW, Dunker AK, Brown CJ, Garner EC, Griswold MD. "Clusterin, a binding protein with a molten globule-like region." Biochemistry. 2001; 40(39): 11828-40. PubMed: 11570883

Comments:
This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.




Region 3
Type:Disordered
Name: 
Location:386 - 447
Length:62
Region sequence:

TVTTHSSDSEVPSRVTEVVVKLFDSDPITVVLPEEVSKDNPKFMDTVAEKALQEYRRKSR
ME

Modification type:  
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Protein detergent
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; Clusterin protein (prepared in PBS) 4 uM)

  2. Nuclear magnetic resonance (NMR)

  3. Sensitivity to proteolysis (310 K; Clusterin (renatured or partially denatured); Deionized H2O (200 uL); Tris (pH 7.3, 10 uL) 1 M; Trypsin (4 ug))

References:
  1. Bailey RW, Dunker AK, Brown CJ, Garner EC, Griswold MD. "Clusterin, a binding protein with a molten globule-like region." Biochemistry. 2001; 40(39): 11828-40. PubMed: 11570883

Comments:
This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.




References

  1. Barghorn S, Davies P, Mandelkow E. "Tau paired helical filaments from Alzheimer's disease brain and assembled in vitro are based on beta-structure in the core domain." Biochemistry. 2004; 43(6): 1694-703. PubMed: 14769047

  2. Tsuruta JK, Wong K, Fritz IB, Griswold MD. "Structural analysis of sulphated glycoprotein 2 from amino acid sequence. Relationship to clusterin and serum protein 40,40." Biochem J. 1990; 268(3): 571-8. PubMed: 2363694



Comments


This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.


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