General information | DisProt: | DP00014 | Name: | Clusterin | Synonym(s): | CLUS_RAT
Sulfated glycoprotein 2
SGP-2
Dimeric acid glycoprotein
DAG
Testosterone repressed prostate message 2
TRPM-2
Clusterin beta chain (cleavage chain 1)
Clusterin alpha chain (cleavage chain 2)
| First appeared in release: | Release 1.0 (08/01/2003) | UniProt: | P05371 | UniGene: | Rn.1780 | SwissProt: | CLUS_RAT | TrEMBL: | | NCBI (GI): | 461756 | Source organism: | Rattus norvegicus (Rat) | Sequence length: | 447 | Percent disordered: | 26% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MKILLLCVAL LLTWDNGMVL GEQEFSDNEL QELSTQGSRY VNKEIQNAVQ GVKHIKTLIE - 60 KTNAERKSLL NSLEEAKKKK EGALDDTRDS EMKLKAFPEV CNETMMALWE ECKPCLKHTC - 120 MKFYARVCRS GSGLVGRQLE EFLNQSSPFY FWMNGDRIDS LLESDRQQSQ VLDAMQDSFT - 180 RASGIIDTLF QDRFFTHEPQ DIHHFSPMGF PHKRPHFLYP KSRLVRSLMP LSHYGPLSFH - 240 NMFQPFFDMI HQAQQAMDVQ LHSPALQFPD VDFLKEGEDD PTVCKEIRHN STGCLKMKGQ - 300 CEKCQEILSV DCSTNNPAQA NLRQELNDSL QVAERLTQQY NELLHSLQSK MLNTSSLLEQ - 360 LNDQFTWVSQ LANLTQGDDQ YLRVSTVTTH SSDSEVPSRV TEVVVKLFDS DPITVVLPEE - 420 VSKDNPKFMD TVAEKALQEY RRKSRME
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Functional narrative |
Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered | Name: | | Location: | 22 - 41 | Length: | 20 | Region sequence: |
EQEFSDNELQELSTQGSRYV | Modification type: | | PDB: | | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Protein detergent
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; Clusterin protein (prepared in PBS) 4 uM)
- Nuclear magnetic resonance (NMR)
- Sensitivity to proteolysis (310 K; Clusterin (renatured or partially denatured); Deionized H2O (200 uL); Tris (pH 7.3, 10 uL) 1 M; Trypsin (4 ug))
| References:
- Bailey RW, Dunker AK, Brown CJ, Garner EC, Griswold MD. "Clusterin, a binding protein with a molten globule-like region." Biochemistry. 2001; 40(39): 11828-40. PubMed: 11570883
| Comments:This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.
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Region 2 | Type: | Disordered | Name: | | Location: | 66 - 97 | Length: | 32 | Region sequence: |
RKSLLNSLEEAKKKKEGALDDTRDSEMKLKAF | Modification type: | | PDB: | | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Protein detergent
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; Clusterin protein (prepared in PBS) 4 uM)
- Nuclear magnetic resonance (NMR)
- Sensitivity to proteolysis (310 K; Clusterin (renatured or partially denatured); Deionized H2O (200 uL); Tris (pH 7.3, 10 uL) 1 M; Trypsin (4 ug))
| References:
- Bailey RW, Dunker AK, Brown CJ, Garner EC, Griswold MD. "Clusterin, a binding protein with a molten globule-like region." Biochemistry. 2001; 40(39): 11828-40. PubMed: 11570883
| Comments:This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.
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Region 3 | Type: | Disordered | Name: | | Location: | 386 - 447 | Length: | 62 | Region sequence: |
TVTTHSSDSEVPSRVTEVVVKLFDSDPITVVLPEEVSKDNPKFMDTVAEKALQEYRRKSR ME | Modification type: | | PDB: | | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Protein detergent
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; Clusterin protein (prepared in PBS) 4 uM)
- Nuclear magnetic resonance (NMR)
- Sensitivity to proteolysis (310 K; Clusterin (renatured or partially denatured); Deionized H2O (200 uL); Tris (pH 7.3, 10 uL) 1 M; Trypsin (4 ug))
| References:
- Bailey RW, Dunker AK, Brown CJ, Garner EC, Griswold MD. "Clusterin, a binding protein with a molten globule-like region." Biochemistry. 2001; 40(39): 11828-40. PubMed: 11570883
| Comments:This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.
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References |
- Barghorn S, Davies P, Mandelkow E. "Tau paired helical filaments from Alzheimer's disease brain and assembled in vitro are based on beta-structure in the core domain." Biochemistry. 2004; 43(6): 1694-703. PubMed: 14769047
- Tsuruta JK, Wong K, Fritz IB, Griswold MD. "Structural analysis of sulphated glycoprotein 2 from amino acid sequence. Relationship to clusterin and serum protein 40,40." Biochem J. 1990; 268(3): 571-8. PubMed: 2363694
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Comments |
This protein has been determined disordered based on numerous experimental results that indicate it contains flexible loops. However, no exact amino acid locations of disorder are known and the domains specified were based on PONDR predictions.
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