| General information | | DisProt: | DP00020 | | Name: | DNA-binding protein RAP1 | | Synonym(s): | RAP1_YEAST
SBF-E
Repressor/activator site-binding protein
TUF
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | P11938 | | UniGene: | | | SwissProt: | RAP1_YEAST | | TrEMBL: | | | NCBI (GI): | 730473 | | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | | Sequence length: | 827 | | Percent disordered: | 2% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MSSPDDFETA PAEYVDALDP SMVVVDSGSA AVTAPSDSAA EVKANQNEEN TGATAAETSE - 60
KVDQTEVEKK DDDDTTEVGV TTTTPSIADT AATANIASTS GASVTEPTTD DTAADEKKEQ - 120
VSGPPLSNMK FYLNRDADAH DSLNDIDQLA RLIRANGGEV LDSKPRESKE NVFIVSPYNH - 180
TNLPTVTPTY IKACCQSNSL LNMENYLVPY DNFREVVDSR LQEESHSNGV DNSNSNSDNK - 240
DSIRPKTEII STNTNGATED STSEKVMVDA EQQARLQEQA QLLRQHVSST ASITSGGHND - 300
LVQIEQPQKD TSNNNNSNVN DEDNDLLTQD NNPQTADEGN ASFQAQRSMI SRGALPSHNK - 360
ASFTDEEDEF ILDVVRKNPT RRTTHTLYDE ISHYVPNHTG NSIRHRFRVY LSKRLEYVYE - 420
VDKFGKLVRD DDGNLIKTKV LPPSIKRKFS ADEDYTLAIA VKKQFYRDLF QIDPDTGRSL - 480
ITDEDTPTAI ARRNMTMDPN HVPGSEPNFA AYRTQSRRGP IAREFFKHFA EEHAAHTENA - 540
WRDRFRKFLL AYGIDDYISY YEAEKAQNRE PEPMKNLTNR PKRPGVPTPG NYNSAAKRAR - 600
NYSSQRNVQP TANAASANAA AAAAAAASNS YAIPENELLD EDTMNFISSL KNDLSNISNS - 660
LPFEYPHEIA EAIRSDFSNE DIYDNIDPDT ISFPPKIATT DLFLPLFFHF GSTRQFMDKL - 720
HEVISGDYEP SQAEKLVQDL CDETGIRKNF STSILTCLSG DLMVFPRYFL NMFKDNVNPP - 780
PNVPGIWTHD DDESLKSNDQ EQIRKLVKKH GTGRMEMRKR FFEKDLL
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| Functional narrative |
Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C1-3A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers. RAP1 associates with SIR3 and SIR4 proteins to form a DNA-binding complex that initiates the repression at the HM loci and telomeres. May also target the binding of RIF1 and RIF2 to silencers and telomeres. Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | Domain 2 | | Location: | 565 - 571 | | Length: | 7 | | Region sequence: |
KAQNREP | | Modification type: | Complex
Native
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Transactivation (transcriptional activation)
Protein-DNA binding
| Detection methods:
- X-ray crystallography (100 K; pH: 6; KCl 20 mM; MES 20 mM; MPD 30 %; resolution 2.2 A; spermine 2 mM)
| References:
- Konig P, Giraldo R, Chapman L, Rhodes D. "The crystal structure of the DNA-binding domain of yeast RAP1 in complex with telomeric DNA." Cell. 1996; 85(1): 125-36. PubMed: 8620531
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | Domain 2 | | Location: | 579 - 585 | | Length: | 7 | | Region sequence: |
NRPKRPG | | Modification type: | Complex
Native
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Transactivation (transcriptional activation)
Protein-protein binding
| Detection methods:
- X-ray crystallography (100 K; pH: 6; KCl 20 mM; MES 20 mM; MPD 30 %; resolution 2.2 A; spermine 2 mM)
| References:
- Konig P, Giraldo R, Chapman L, Rhodes D. "The crystal structure of the DNA-binding domain of yeast RAP1 in complex with telomeric DNA." Cell. 1996; 85(1): 125-36. PubMed: 8620531
| Comments:
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| References |
- Konig P, Giraldo R, Chapman L, Rhodes D. "The crystal structure of the DNA-binding domain of yeast RAP1 in complex with telomeric DNA." Cell. 1996; 85(1): 125-36. PubMed: 8620531
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| Comments |
sent for AV (6-15-2010) PubMed: 8620531
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