DP00029: Glial cell line-derived neurotrophic factorFASTA viewXML view

General information
DisProt:DP00029
Name:Glial cell line-derived neurotrophic factor
Synonym(s):GDNF_RAT
GDNF
Astrocyte-derived trophic factor
ATF
First appeared in release:Release 2.0 (02/14/2005)
UniProt:Q07731
UniGene: 
SwissProt: GDNF_RAT
TrEMBL:  
NCBI (GI): 729568
Source organism:Rattus norvegicus (Rat)
Sequence length:211
Percent disordered:19%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MKLWDVVAVC LVLLHTASAF PLPAGKRLLE APAEDHSLGH RRVPFALTSD SNMPEDYPDQ - 60
FDDVMDFIQA TIKRLKRSPD KQAAALPRRE RNRQAAAASP ENSRGKGRRG QRGKNRGCVL - 120
TAIHLNVTDL GLGYETKEEL IFRYCSGSCE AAETMYDKIL KNLSRSRRLT SDKVGQACCR - 180
PVAFDDDLSF LDDSLVYHIL RKHSAKRCGC I



Functional narrative    

GDNF is a secreted protein that is important in brain functions. It increases the chance of survival of dopaminergic neurons and increases their uptake of dopamine. It has been shown in the lab to restore cell function with induced Parkinson’s disease symptoms. Morphologic differentiation of neurons has been linked to GDNF as well. Some studies have also shown that it is important in kidney growth and development. It is a member of the transforming growth factor-beta family.

Region 1: 77-113 Region 3: 114-169 Region 2: 170-173 Region 4: 174-211

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name: 
Location:77 - 113
Length:37
Region sequence:

RSPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRG

Modification type: Engineered
PDB: 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D
Structural/functional type: Relationship to function unknown
Functional classes: Molecular assembly
Functional subclasses: Protein-protein binding
Detection methods:
  1. X-ray crystallography (benzamidine 0.1 mM; HEPES (pH 7.0) 50 mM; LiCl 0.8 M; PEG 6000 20 %; soduim azide 0.02 %)

References:
  1. Eigenbrot C, Gerber N. "X-ray structure of glial cell-derived neurotrophic factor at 1.9 A resolution and implications for receptor binding." Nat Struct Biol. 1997; 4(6): 435-8. PubMed: 9187648

  2. Eketjäll S, Fainzilber M, Murray-Rust J, Ibáñez CF. "Distinct structural elements in GDNF mediate binding to GFRalpha1 and activation of the GFRalpha1-c-Ret receptor complex." EMBO J. 1999; 18(21): 5901-10. PubMed: 10545102

Comments:
The first identifiable residue in 1AGQ was ASN 115 in chain A, LYS 114 in chain B, GLY 117 in chain C, and GLY 117 in chain D. This supports claims in the Eigenbrot paper about dependency on the monomer.


The experimental protein consisted of residues 78 - 211 of SwissProt entry Q07731.




Region 2
Type:Disordered - Extended
Name: 
Location:170 - 173
Length:4
Region sequence:

TSDK

Modification type: Engineered
PDB: 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (benzamidine 0.1 mM; HEPES (pH 7.0) 50 mM; LiCl 0.8 M; PEG 6000 20 %; soduim azide 0.02 %)

References:
  1. Eketjäll S, Fainzilber M, Murray-Rust J, Ibáñez CF. "Distinct structural elements in GDNF mediate binding to GFRalpha1 and activation of the GFRalpha1-c-Ret receptor complex." EMBO J. 1999; 18(21): 5901-10. PubMed: 10545102

Comments:
This sequence is part of a flexible loop that connects the alpha helix region to the second finger region of the protein.


The experimental protein consisted of residues 78 - 211 of SwissProt entry Q07731.




Region 3
Type:Ordered
Name: 
Location:114 - 169
Length:56
Region sequence:

KNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCEAAETMYDKILKNLSRSRRL

Modification type:  
PDB: 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Eigenbrot C, Gerber N. "X-ray structure of glial cell-derived neurotrophic factor at 1.9 A resolution and implications for receptor binding." Nat Struct Biol. 1997; 4(6): 435-8. PubMed: 9187648

Comments:
 



Region 4
Type:Ordered
Name: 
Location:174 - 211
Length:38
Region sequence:

VGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGCI

Modification type:  
PDB: 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Eigenbrot C, Gerber N. "X-ray structure of glial cell-derived neurotrophic factor at 1.9 A resolution and implications for receptor binding." Nat Struct Biol. 1997; 4(6): 435-8. PubMed: 9187648

Comments:
 



References

  1. Lin LF, Doherty DH, Lile JD, Bektesh S, Collins F. "GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons." Science. 1993; 260(5111): 1130-2. PubMed: 8493557


If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us.


Disprot-footer
Contact us