General information | DisProt: | DP00029 | Name: | Glial cell line-derived neurotrophic factor | Synonym(s): | GDNF_RAT
GDNF
Astrocyte-derived trophic factor
ATF
| First appeared in release: | Release 2.0 (02/14/2005) | UniProt: | Q07731 | UniGene: | | SwissProt: | GDNF_RAT | TrEMBL: | | NCBI (GI): | 729568 | Source organism: | Rattus norvegicus (Rat) | Sequence length: | 211 | Percent disordered: | 19% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MKLWDVVAVC LVLLHTASAF PLPAGKRLLE APAEDHSLGH RRVPFALTSD SNMPEDYPDQ - 60 FDDVMDFIQA TIKRLKRSPD KQAAALPRRE RNRQAAAASP ENSRGKGRRG QRGKNRGCVL - 120 TAIHLNVTDL GLGYETKEEL IFRYCSGSCE AAETMYDKIL KNLSRSRRLT SDKVGQACCR - 180 PVAFDDDLSF LDDSLVYHIL RKHSAKRCGC I
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Functional narrative |
GDNF is a secreted protein that is important in brain functions. It increases the chance of survival of dopaminergic neurons and increases their uptake of dopamine. It has been shown in the lab to restore cell function with induced Parkinson’s disease symptoms. Morphologic differentiation of neurons has been linked to GDNF as well. Some studies have also shown that it is important in kidney growth and development. It is a member of the transforming growth factor-beta family.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered - Extended | Name: | | Location: | 77 - 113 | Length: | 37 | Region sequence: |
RSPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRG | Modification type: | Engineered
| PDB: | 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D | Structural/functional type: | Relationship to function unknown | Functional classes: | Molecular assembly
| Functional subclasses: | Protein-protein binding
| Detection methods:
- X-ray crystallography (benzamidine 0.1 mM; HEPES (pH 7.0) 50 mM; LiCl 0.8 M; PEG 6000 20 %; soduim azide 0.02 %)
| References:
- Eigenbrot C, Gerber N. "X-ray structure of glial cell-derived neurotrophic factor at 1.9 A resolution and implications for receptor binding." Nat Struct Biol. 1997; 4(6): 435-8. PubMed: 9187648
- Eketjäll S, Fainzilber M, Murray-Rust J, Ibáñez CF. "Distinct structural elements in GDNF mediate binding to GFRalpha1 and activation of the GFRalpha1-c-Ret receptor complex." EMBO J. 1999; 18(21): 5901-10. PubMed: 10545102
| Comments:The first identifiable residue in 1AGQ was ASN 115 in chain A, LYS 114 in chain B, GLY 117 in chain C, and GLY 117 in chain D. This supports claims in the Eigenbrot paper about dependency on the monomer.
The experimental protein consisted of residues 78 - 211 of SwissProt entry Q07731.
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Region 2 | Type: | Disordered - Extended | Name: | | Location: | 170 - 173 | Length: | 4 | Region sequence: |
TSDK | Modification type: | Engineered
| PDB: | 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (benzamidine 0.1 mM; HEPES (pH 7.0) 50 mM; LiCl 0.8 M; PEG 6000 20 %; soduim azide 0.02 %)
| References:
- Eketjäll S, Fainzilber M, Murray-Rust J, Ibáñez CF. "Distinct structural elements in GDNF mediate binding to GFRalpha1 and activation of the GFRalpha1-c-Ret receptor complex." EMBO J. 1999; 18(21): 5901-10. PubMed: 10545102
| Comments:This sequence is part of a flexible loop that connects the alpha helix region to the second finger region of the protein.
The experimental protein consisted of residues 78 - 211 of SwissProt entry Q07731.
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Region 3 | Type: | Ordered | Name: | | Location: | 114 - 169 | Length: | 56 | Region sequence: |
KNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCEAAETMYDKILKNLSRSRRL | Modification type: | | PDB: | 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D | Structural/functional type: | | Functional classes: | | Functional subclasses: | | Detection methods: | References:
- Eigenbrot C, Gerber N. "X-ray structure of glial cell-derived neurotrophic factor at 1.9 A resolution and implications for receptor binding." Nat Struct Biol. 1997; 4(6): 435-8. PubMed: 9187648
| Comments:
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Region 4 | Type: | Ordered | Name: | | Location: | 174 - 211 | Length: | 38 | Region sequence: |
VGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGCI | Modification type: | | PDB: | 1AGQ:A, 1AGQ:B, 1AGQ:C, 1AGQ:D | Structural/functional type: | | Functional classes: | | Functional subclasses: | | Detection methods: | References:
- Eigenbrot C, Gerber N. "X-ray structure of glial cell-derived neurotrophic factor at 1.9 A resolution and implications for receptor binding." Nat Struct Biol. 1997; 4(6): 435-8. PubMed: 9187648
| Comments:
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References |
- Lin LF, Doherty DH, Lile JD, Bektesh S, Collins F. "GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons." Science. 1993; 260(5111): 1130-2. PubMed: 8493557
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