Annotations for this protein have been verified by the authors of the corresponding papers


DP00031: Glycine N-methyltransferaseFASTA viewXML view

General information
DisProt:DP00031
Name:Glycine N-methyltransferase
Synonym(s):GNMT_RAT
Folate-binding protein
EC=2.1.1.20
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P13255
UniGene:Rn.11142
SwissProt: GNMT_RAT
TrEMBL:  
NCBI (GI): 121328
Source organism:Rattus norvegicus (Rat)
Sequence length:292
Percent disordered:14%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
VDSVYRTRSL GVAAEGIPDQ YADGEAARVW QLYIGDTRSR TAEYKAWLLG LLRQHGCHRV - 60
LDVACGTGVD SIMLVEEGFS VTSVDASDKM LKYALKERWN RRKEPAFDKW VIEEANWLTL - 120
DKDVPAGDGF DAVICLGNSF AHLPDSKGDQ SEHRLALKNI ASMVRPGGLL VIDHKNYDYI - 180
LSTGCAPPGK NIYYKSDLTK DITTSVLTVN NKAHMVTLDY TVQVPGAGRD GAPGFSKFRL - 240
SYYPHCLASF TELVQEAFGG RCQHSVLGDF KPYRPGQAYV PCYFIHVLKK TG

Region 1: 1-40

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:N-terminal domain
Location:1 - 40
Length:40
Region sequence:

VDSVYRTRSLGVAAEGIPDQYADGEAARVWQLYIGDTRSR

Modification type: Complex
Mutant
PDB: 1D2H:A, 1D2H:B, 1D2H:C, 1D2H:D
Structural/functional type:  
Functional classes:  
Functional subclasses: Methylation
Detection methods:
  1. X-ray crystallography (93 K; pH: 5.6; ethylene glycol (v/v) 20 %; NaCl 50 mM; PEG 3400 10 %; resolution 3 A; sodium citrate 100 mM)
References:
  1. Huang Y, Komoto J, Konishi K, Takata Y, Ogawa H, Gomi T, Fujioka M, Takusagawa F. "Mechanisms for auto-inhibition and forced product release in glycine N-methyltransferase: crystal structures of wild-type, mutant R175K and S-adenosylhomocysteine-bound R175K enzymes." J Mol Biol. 2000; 298(1): 149-62. PubMed: 10756111
Comments:
 



References

  1. Huang Y, Komoto J, Konishi K, Takata Y, Ogawa H, Gomi T, Fujioka M, Takusagawa F. "Mechanisms for auto-inhibition and forced product release in glycine N-methyltransferase: crystal structures of wild-type, mutant R175K and S-adenosylhomocysteine-bound R175K enzymes." J Mol Biol. 2000; 298(1): 149-62. PubMed: 10756111



Comments


AV 6-28-2010 PubMed: 10756111


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