| General information | | DisProt: | DP00046 | | Name: | Lymphoid enhancer-binding factor 1 | | Synonym(s): | LEF1_RAT
LEF-1
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | Q9QXN1 | | UniGene: | Rn.21926 | | SwissProt: | LEF1_RAT | | TrEMBL: | | | NCBI (GI): | 8928168 | | Source organism: | Rattus norvegicus (Rat) | | Sequence length: | 397 | | Percent disordered: | 26% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MPQLSGGGGG GDPELCATDE MIPFKDEGDP QKEKIFAEIS HPEEEGDLAD IKSSLVNESE - 60
IIPASNGHEV VGQTQSSQEP YHDKAREHPD DGKHPDGGLY NKGPSYSSYS GYIMMPNMNS - 120
DPYMSNGSLS PPIPRTSNKV PVVQPSHAVH PLTPLITYSD EHFSPGSHPS HIPSEVNPKQ - 180
GMSRHPPAPE MPTFYPLSPG GVGQITPPLG WQGQPVYPIT GGFRQAYPSS LSGDTSMSRF - 240
SHHMIPGPPG PHTTGIPHPA IVTPQVKQEH PHTDSDLMHV KPEHEQRKEQ EPKRPHIKKP - 300
LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL - 360
YPGWSARDNY GKKKKRKREK LQESTSGTGP RMTAAYI
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| Functional narrative |
Lymphoid enhancer-binding factor-1 is a sequence-specific and cell-type-specific DNA binding protein that is involved in the Wnt signaling pathway. Regulation of T-cell receptor alpha enhancer function is controlled by the TCF/LEF family. Lef-1 is also an architectural protein that facilitates the binding of other transcription factors to the corresponding DNA sequence to activate the transcription of target genes. In the presence of CTNNB1 and EP300 a complex is formed to facilitate gene transcription. The HMG domain (residues 279 – 365) plus the remaining C-terminal end contain the DNA-bending and protein-DNA binding disordered amino acids.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | | | Location: | 296 - 397 | | Length: | 102 | | Region sequence: |
HIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQ
LHMQLYPGWSARDNYGKKKKRKREKLQESTSGTGPRMTAAYI | | Modification type: | | | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | | | Functional subclasses: | DNA bending
Protein-DNA binding
| Detection methods:
- Nuclear magnetic resonance (NMR)
| References:
- Love JJ, Li X, Case DA, Giese K, Grosschedl R, Wright PE. "Structural basis for DNA bending by the architectural transcription factor LEF-1." Nature. 1995; 376(6543): 791-795. PubMed: 7651541
| Comments:
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| References |
- Kobielak K, Kobielak A, Trzeciak WH. "Cloning of the lymphoid enhancer binding factor-1 (Lef-1) cDNA from rat kidney: homology to the mouse sequence." Acta Biochim Pol. 1999; 46(4): 885-888. PubMed: 10824856
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