| General information | | DisProt: | DP00050 | | Name: | Neurofilament heavy polypeptide | | Synonym(s): | NFH_MOUSE
NF-H
Neurofilament H
Neurofilament triplet H protein
200 kDa neurofilament protein
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | P19246 | | UniGene: | Mm.298283 | | SwissProt: | NFH_MOUSE | | TrEMBL: | | | NCBI (GI): | 94730399 | | Source organism: | Mus musculus (Mouse) | | Sequence length: | 1087 | | Percent disordered: | 62% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MSFGSADALL GAPFAPLHGG GSLHYSLSRK AGPGGTRSAA GSSSGFHSWA RTSVSSVSAS - 60
PSRFRGAASS TDSLDTLSNG PEGCVVAAVA ARSEKEQLQA LNDRFAGYID KVRQLEAHNR - 120
SLEGEAAALR QQKGRAAMGE LYEREVREMR GAVLRLGAAR GQLRLEQEHL LEDIAHVRQR - 180
LDEEARQREE AEAAARALAF AQEAEAARVE LQKKAQALQE ECGYLRRHHQ EEVGELLGQI - 240
QGCGAAQAQA QAEARDALKC DVTSALREIR AQLEGHAVQS SLQSEEWFRV RLDRLSEAAK - 300
VNTDAMRSAQ EEITEYRRQL QARTTELEAL KSTKESLERQ RSELEDRHQA DIASYQDAIQ - 360
QLDSELRNTK WEMAAQLREY QDLLNVKMAL DIEIAAYRKL LEGEECRIGF GPSPFSLTEG - 420
LPKIPSISTH IKVKSEEMIK VVEKSEKETV IVEGQTEEIR VTEGVTEEED KEAQGQEGEE - 480
AEEGEEKEEE ELAAATSPPA EEAASPEKET KSRVKEEAKS PGEAKSPGEA KSPAEAKSPG - 540
EAKSPGEAKS PGEAKSPAEP KSPAEPKSPA EAKSPAEPKS PATVKSPGEA KSPSEAKSPA - 600
EAKSPAEAKS PAEAKSPAEA KSPAEAKSPA EAKSPATVKS PGEAKSPSEA KSPAEAKSPA - 660
EAKSPAEAKS PAEVKSPGEA KSPAEPKSPA EAKSPAEVKS PAEAKSPAEV KSPGEAKSPA - 720
AVKSPAEAKS PAAVKSPGEA KSPGEAKSPA EAKSPAEAKS PIEVKSPEKA KTPVKEGAKS - 780
PAEAKSPEKA KSPVKEDIKP PAEAKSPEKA KSPVKEGAKP PEKAKPLDVK SPEAQTPVQE - 840
EATVPTDIRP PEQVKSPAKE KAKSPEKEEA KTSEKVAPKK EEVKSPVKEE VKAKEPPKKV - 900
EEEKTLPTPK TEAKESKKDE APKEAPKPKV EEKKETPTEK PKDSTAEAKK EEAGEKKKAV - 960
ASEEETPAKL GVKEEAKPKE KTETTKTEAE DTKAKEPSKP TETEKPKKEE MPAAPEKKDT - 1020
KEEKTTESRK PEEKPKMEAK VKEDDKSLSK EPSKPKTEKA EKSSSTDQKE SQPPEKTTED - 1080
KATKGEK
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| Functional narrative |
Neurofilament triplet H protein (NF-H) is one of three neurofilament proteins that create the major cytoskelatal elements that control axonal caliber. The C-terminal domain contains an entropic bristle side arms that extend ~85nm from the core of the protein. The thermally driven motion of the side arms form an entropic brush. The long-range interactions produced by the brush maintain spacing between neighboring filaments. Entropic brushes exclude large molecules but allow entry to water molecules and small solutes. Accumulation of filaments can cause toxic neurodegenerative diseases such as amyotrophic lateral sclerosis.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 409 - 1087 | | Length: | 679 | | Region sequence: |
GFGPSPFSLTEGLPKIPSISTHIKVKSEEMIKVVEKSEKETVIVEGQTEEIRVTEGVTEE
EDKEAQGQEGEEAEEGEEKEEEELAAATSPPAEEAASPEKETKSRVKEEAKSPGEAKSPG
EAKSPAEAKSPGEAKSPGEAKSPGEAKSPAEPKSPAEPKSPAEAKSPAEPKSPATVKSPG
EAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAKSPATVKSPGEAKSPS
EAKSPAEAKSPAEAKSPAEAKSPAEVKSPGEAKSPAEPKSPAEAKSPAEVKSPAEAKSPA
EVKSPGEAKSPAAVKSPAEAKSPAAVKSPGEAKSPGEAKSPAEAKSPAEAKSPIEVKSPE
KAKTPVKEGAKSPAEAKSPEKAKSPVKEDIKPPAEAKSPEKAKSPVKEGAKPPEKAKPLD
VKSPEAQTPVQEEATVPTDIRPPEQVKSPAKEKAKSPEKEEAKTSEKVAPKKEEVKSPVK
EEVKAKEPPKKVEEEKTLPTPKTEAKESKKDEAPKEAPKPKVEEKKETPTEKPKDSTAEA
KKEEAGEKKKAVASEEETPAKLGVKEEAKPKEKTETTKTEAEDTKAKEPSKPTETEKPKK
EEMPAAPEKKDTKEEKTTESRKPEEKPKMEAKVKEDDKSLSKEPSKPKTEKAEKSSSTDQ
KESQPPEKTTEDKATKGEK | | Modification type: | | | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
- SDS-PAGE gel, Aberrant mobility on
- Sensitivity to proteolysis
| References:
- Brown HG, Hoh JH. "Entropic exclusion by neurofilament sidearms: a mechanism for maintaining interfilament spacing." Biochemistry. 1997; 36(49): 15035-40. PubMed: 9424114
- Hoh JH. "Functional protein domains from the thermally driven motion of polypeptide chains: a proposal." Proteins. 1998; 32(2): 223-8. PubMed: 9714161
- Julien JP, Cote F, Beaudet L, Sidky M, Flavell D, Grosveld F, Mushynski W. "Sequence and structure of the mouse gene coding for the largest neurofilament subunit." Gene. 1988; 68(2): 307-314. PubMed: 3220257
| Comments:According to Brown (1997), the disordered region is a portion of the C-terminus. However, the exact numbered amino acids are not documented in the literature.
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