DP00051: Ornithine decarboxylaseFASTA viewXML view

General information
DisProt:DP00051
Name:Ornithine decarboxylase
Synonym(s):DCOR_TRYBB
ODC
L-ornithine carboxy-lyase
EC=4.1.1.17
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P07805
UniGene: 
SwissProt: DCOR_TRYBB
TrEMBL:  
NCBI (GI): 7404357
Source organism:Trypanosoma brucei brucei
Sequence length:425
Percent disordered:18%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
GAMDIVVNDD LSCRFLEGFN TRDALCKKIS MNTCDEGDPF FVADLGDIVR KHETWKKCLP - 60
RVTPFYAVAC NDDWRVLGTL AALGTGFDCA SNTEIQRVRG IGVPPEKIIY ANPCKQISHI - 120
RYARDSGVDV MTFDCVDELE KVAKTHPKAK MVLRISTDDS LARCRLSVKF GAKVEDCRFI - 180
LEQAKKLNID VTGVSFHVGS GSTDASTFAQ AISDSRFVFD MGTELGFNMH ILDIGGGFPG - 240
TRDAPLKFEE IAGVINNALE KHFPPDLKLT IVAEPGRYYV ASAFTLAVNV IAKKVTPGVQ - 300
TDVGAHAESN AQSFMYYVND GVYGSFNCIL YDHAVVRPLP QREPIPNEKL YPSSVWGPTC - 360
DGLDQIVERY YLPEMQVGEW LLFEDMGAYT VVGTSSFNGF QSPTIYYVVS GLPDHVVREL - 420
KSQKS



Functional narrative    

Ornithine decarboxylase (ODC) is a pyridoxal 5'-phosphate (PLP) dependent homodimeric enzyme, which belongs to the Orn/Lys/Arg decarboxylase class-II family. It is a recognized drug target against African sleeping sickness in the host Trypanosoma brucei. ODC catalyzes the first committed (rate-determining) step in the biosynthetic pathway of the polyamines. Polyamines are ubiquitous to all cells and are required for cell growth and differentiation. The functions of the disordered regions are not known.

Region 5: 1-13 Region 1: 1-35 Region 6: 160-164 Region 2: 158-165 Region 3: 298-310 Region 7: 297-311 Region 8: 409-425 Region 4: 412-425

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name: 
Location:1 - 35
Length:35
Region sequence:

GAMDIVVNDDLSCRFLEGFNTRDALCKKISMNTCD

Modification type: Engineered
PDB: 2TOD:B, 1QU4:A, 1QU4:B, 1QU4:C, 1QU4:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography

References:
  1. Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800

  2. Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.

Comments:
PDB entry 2TOD documents residues 1-36 being absent from the electron density map. PDB entry 1QU4 documents 1-34 being absent form the electron density map.




Region 2
Type:Disordered
Name: 
Location:158 - 165
Length:8
Region sequence:

DDSLARCR

Modification type: Engineered
PDB: 2TOD:A, 2TOD:B, 2TOD:C, 2TOD:D, 1QU4:A, 1QU4:B, 1QU4:C, 1QU4:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography

References:
  1. Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800

  2. Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.

Comments:
PDB 1QU4 contains an A69K substitution




Region 3
Type:Disordered
Name: 
Location:298 - 310
Length:13
Region sequence:

GVQTDVGAHAESN

Modification type: Engineered
PDB: 2TOD:A, 2TOD:B, 2TOD:C, 2TOD:D, 1QU4:A, 1QU4:B, 1QU4:C, 1QU4:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography

References:
  1. Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800

  2. Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.

Comments:
PDB 1QU4 contains an A69K substitution




Region 4
Type:Disordered
Name: 
Location:412 - 425
Length:14
Region sequence:

LPDHVVRELKSQKS

Modification type: Engineered
PDB: 2TOD:A, 2TOD:B, 2TOD:C, 2TOD:D, 1AQ4:A, 1AQ4:B, 1AQ4:C, 1AQ4:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography

References:
  1. Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800

  2. Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.

Comments:
2TOD documents amino acids 411-425 as being absent from the density map. PDB entry 1QU4 contains an A69K substitution




Region 5
Type:Disordered
Name: 
Location:1 - 13
Length:13
Region sequence:

GAMDIVVNDDLSC

Modification type: Engineered
PDB: 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (289 K; pH: 7.5; )

References:
  1. Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770

Comments:
The PDB entry documents absence from density map in regions 1-13 and then 31-36.PDB entry 1QU4 contains an A69K substitution




Region 6
Type:Disordered
Name: 
Location:160 - 164
Length:5
Region sequence:

SLARC

Modification type: Engineered
PDB: 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (289 K; pH: 7.5; )

References:
  1. Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770

Comments:
 



Region 7
Type:Disordered
Name: 
Location:297 - 311
Length:15
Region sequence:

PGVQTDVGAHAESNA

Modification type: Engineered
PDB: 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (289 K; pH: 7.5; )

References:
  1. Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770

Comments:
PDB entry 1F3T contains an A69K substitution




Region 8
Type:Disordered
Name: 
Location:409 - 425
Length:17
Region sequence:

VSGLPDHVVRELKSQKS

Modification type: Engineered
PDB: 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (289 K; pH: 7.5; )

References:
  1. Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770

Comments:
1F3T documents 423-425 as being absent from the electron density map. PDB entry 1F3T contains an A69K substitution



If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us.


Disprot-footer
Contact us