| General information | | DisProt: | DP00051 | | Name: | Ornithine decarboxylase | | Synonym(s): | DCOR_TRYBB
ODC
L-ornithine carboxy-lyase
EC=4.1.1.17
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | P07805 | | UniGene: | | | SwissProt: | DCOR_TRYBB | | TrEMBL: | | | NCBI (GI): | 7404357 | | Source organism: | Trypanosoma brucei brucei | | Sequence length: | 425 | | Percent disordered: | 18% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
GAMDIVVNDD LSCRFLEGFN TRDALCKKIS MNTCDEGDPF FVADLGDIVR KHETWKKCLP - 60
RVTPFYAVAC NDDWRVLGTL AALGTGFDCA SNTEIQRVRG IGVPPEKIIY ANPCKQISHI - 120
RYARDSGVDV MTFDCVDELE KVAKTHPKAK MVLRISTDDS LARCRLSVKF GAKVEDCRFI - 180
LEQAKKLNID VTGVSFHVGS GSTDASTFAQ AISDSRFVFD MGTELGFNMH ILDIGGGFPG - 240
TRDAPLKFEE IAGVINNALE KHFPPDLKLT IVAEPGRYYV ASAFTLAVNV IAKKVTPGVQ - 300
TDVGAHAESN AQSFMYYVND GVYGSFNCIL YDHAVVRPLP QREPIPNEKL YPSSVWGPTC - 360
DGLDQIVERY YLPEMQVGEW LLFEDMGAYT VVGTSSFNGF QSPTIYYVVS GLPDHVVREL - 420
KSQKS
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| Functional narrative |
Ornithine decarboxylase (ODC) is a pyridoxal 5'-phosphate (PLP) dependent homodimeric enzyme, which belongs to the Orn/Lys/Arg decarboxylase class-II family. It is a recognized drug target against African sleeping sickness in the host Trypanosoma brucei. ODC catalyzes the first committed (rate-determining) step in the biosynthetic pathway of the polyamines. Polyamines are ubiquitous to all cells and are required for cell growth and differentiation. The functions of the disordered regions are not known.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | | | Location: | 1 - 35 | | Length: | 35 | | Region sequence: |
GAMDIVVNDDLSCRFLEGFNTRDALCKKISMNTCD | | Modification type: | Engineered
| | PDB: | 2TOD:B, 1QU4:A, 1QU4:B, 1QU4:C, 1QU4:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography
| References:
- Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800
- Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.
| Comments:PDB entry 2TOD documents residues 1-36 being absent from the electron density map.
PDB entry 1QU4 documents 1-34 being absent form the electron density map.
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 158 - 165 | | Length: | 8 | | Region sequence: |
DDSLARCR | | Modification type: | Engineered
| | PDB: | 2TOD:A, 2TOD:B, 2TOD:C, 2TOD:D, 1QU4:A, 1QU4:B, 1QU4:C, 1QU4:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography
| References:
- Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800
- Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.
| Comments:PDB 1QU4 contains an A69K substitution
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| Region 3 | | Type: | Disordered | | Name: | | | Location: | 298 - 310 | | Length: | 13 | | Region sequence: |
GVQTDVGAHAESN | | Modification type: | Engineered
| | PDB: | 2TOD:A, 2TOD:B, 2TOD:C, 2TOD:D, 1QU4:A, 1QU4:B, 1QU4:C, 1QU4:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography
| References:
- Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800
- Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.
| Comments:PDB 1QU4 contains an A69K substitution
|
| Region 4 | | Type: | Disordered | | Name: | | | Location: | 412 - 425 | | Length: | 14 | | Region sequence: |
LPDHVVRELKSQKS | | Modification type: | Engineered
| | PDB: | 2TOD:A, 2TOD:B, 2TOD:C, 2TOD:D, 1AQ4:A, 1AQ4:B, 1AQ4:C, 1AQ4:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography
| References:
- Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ. "X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine." Biochemistry. 1999; 38(46): 15174-15184. PubMed: 10563800
- Jancarik J, Kim SH. "Sparse matrix sampling: a screening method for crystallization of proteins." J Appl Cryst. 1991; 24(Pt. 4): 409-11.
| Comments:2TOD documents amino acids 411-425 as being absent from the density map. PDB entry 1QU4 contains an A69K substitution
|
| Region 5 | | Type: | Disordered | | Name: | | | Location: | 1 - 13 | | Length: | 13 | | Region sequence: |
GAMDIVVNDDLSC | | Modification type: | Engineered
| | PDB: | 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (289 K; pH: 7.5; )
| References:
- Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770
| Comments:The PDB entry documents absence from density map in regions 1-13 and then 31-36.PDB entry 1QU4 contains an A69K substitution
|
| Region 6 | | Type: | Disordered | | Name: | | | Location: | 160 - 164 | | Length: | 5 | | Region sequence: |
SLARC | | Modification type: | Engineered
| | PDB: | 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (289 K; pH: 7.5; )
| References:
- Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770
| Comments:
|
| Region 7 | | Type: | Disordered | | Name: | | | Location: | 297 - 311 | | Length: | 15 | | Region sequence: |
PGVQTDVGAHAESNA | | Modification type: | Engineered
| | PDB: | 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (289 K; pH: 7.5; )
| References:
- Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770
| Comments:PDB entry 1F3T contains an A69K substitution
|
| Region 8 | | Type: | Disordered | | Name: | | | Location: | 409 - 425 | | Length: | 17 | | Region sequence: |
VSGLPDHVVRELKSQKS | | Modification type: | Engineered
| | PDB: | 1F3T:A, 1F3T:B, 1F3T:C, 1F3T:D | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (289 K; pH: 7.5; )
| References:
- Jackson LK, Brooks HB, Osterman AL, Goldsmith EJ, Phillips MA. "Altering the reaction specificity of eukaryotic ornithine decarboxylase." Biochemistry. 2000; 39(37): 11247-11257. PubMed: 10985770
| Comments:1F3T documents 423-425 as being absent from the electron density map.
PDB entry 1F3T contains an A69K substitution
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