Annotation for this protein is in progress - please check future releases for more complete information


DP00055: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1FASTA viewXML view

General information
DisProt:DP00055
Name:1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1
Synonym(s):PLCD1_RAT
Phosphoinositide phospholipase C
Phosphatidylinositol phospholipase C
Phospholipase C-delta-1
PLC-delta-1
PLC-III
1-phosphatidyl-D-myo-inositol-4,5-bisphosphate inositoltrisphosphohydrolase
Monophosphatidylinositol phosphodiesterase
PI-PLC
Triphosphoinositide phosphodiesterase
EC 3.1.4.11
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P10688
UniGene:Rn.12324
SwissProt: PLCD1_RAT
TrEMBL:  
NCBI (GI): 130228
Source organism:Rattus norvegicus (Rat)
Sequence length:756
Percent disordered:20%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MDSGRDFLTL HGLQDDPDLQ ALLKGSQLLK VKSSSWRRER FYKLQEDCKT IWQESRKVMR - 60
SPESQLFSIE DIQEVRMGHR TEGLEKFARD IPEDRCFSIV FKDQRNTLDL IAPSPADAQH - 120
WVQGLRKIIH HSGSMDQRQK LQHWIHSCLR KADKNKDNKM NFKELKDFLK ELNIQVDDGY - 180
ARKIFRECDH SQTDSLEDEE IETFYKMLTQ RAEIDRAFEE AAGSAETLSV ERLVTFLQHQ - 240
QREEEAGPAL ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGNAFSLA HRRVYQDMDQ - 300
PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ EPIIYHGYTF - 360
TSKILFCDVL RAIRDYAFKA SPYPVILSLE NHCSLEQQRV MARHLRAILG PILLDQPLDG - 420
VTTSLPSPEQ LKGKILLKGK KLGGLLPAGG ENGSEATDVS DEVEAAEMED EAVRSQVQHK - 480
PKEDKLKLVP ELSDMIIYCK SVHFGGFSSP GTSGQAFYEM ASFSESRALR LLQESGNGFV - 540
RHNVSCLSRI YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGPEMDVYL GCFQDNGGCG - 600
YVLKPAFLRD PNTTFNSRAL TQGPWWRPER LRVRIISGQQ LPKVNKNKNS IVDPKVIVEI - 660
HGVGRDTGSR QTAVITNNGF NPRWDMEFEF EVTVPDLALV RFMVEDYDSS SKNDFIGQST - 720
IPWNSLKQGY RHVHLLSKNG DQHPSATLFV KISIQD

Region 1: 135-206 Region 2: 445-485 Region 6: 446-488 Region 5: 502-517 Region 7: 645-650 Region 3: 643-653 Region 4: 706-714

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:135 - 206
Length:72
Region sequence:

MDQRQKLQHWIHSCLRKADKNKDNKMNFKELKDFLKELNIQVDDGYARKIFRECDHSQTD
SLEDEEIETFYK

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography
References:
  1. Grobler JA, Essen LO, Williams RL, Hurley JH. "C2 domain conformational changes in phospholipase C-delta 1." Nat Struct Biol. 1996; 3(9): 788-95. PubMed: 8784353
Comments:
 



Region 2
Type:Disordered
Name: 
Location:445 - 485
Length:41
Region sequence:

LLPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHKPKEDK

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography
References:
  1. Grobler JA, Essen LO, Williams RL, Hurley JH. "C2 domain conformational changes in phospholipase C-delta 1." Nat Struct Biol. 1996; 3(9): 788-95. PubMed: 8784353
Comments:
 



Region 3
Type:Disordered
Name: 
Location:643 - 653
Length:11
Region sequence:

KVNKNKNSIVD

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography
References:
  1. Grobler JA, Essen LO, Williams RL, Hurley JH. "C2 domain conformational changes in phospholipase C-delta 1." Nat Struct Biol. 1996; 3(9): 788-95. PubMed: 8784353
Comments:
 



Region 4
Type:Disordered
Name: 
Location:706 - 714
Length:9
Region sequence:

DYDSSSKND

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography
References:
  1. Grobler JA, Essen LO, Williams RL, Hurley JH. "C2 domain conformational changes in phospholipase C-delta 1." Nat Struct Biol. 1996; 3(9): 788-95. PubMed: 8784353
Comments:
 



Region 5
Type:Disordered
Name:Catalytic domain loop
Location:502 - 517
Length:16
Region sequence:

VHFGGFSSPGTSGQAF

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Metal binding
Substrate/ligand binding
Detection methods:
  1. X-ray crystallography (100 K; ammonium phosphate 1.4 M; CHAPSO (w/v) 0.1 %; DTT 1 mM; EGTA 0.5 mM; NaCl 100 mM; NaN3 (w/v) 0.01 %; protein (sample) 10 mg/mL; Tris-HCl 10 mM)
References:
  1. Essen LO, Perisic O, Cheung R, Katan M, Williams RL. "Crystal structure of a mammalian phosphoinositide-specific phospholipase C delta." Nature. 1996; 380(6575): 595-602. PubMed: 8602259
Comments:
 



Region 6
Type:Disordered
Name: 
Location:446 - 488
Length:43
Region sequence:

LPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHKPKEDKLKL

Modification type: Fragment
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Metal binding
Flexible linkers/spacers
Substrate/ligand binding
Detection methods:
  1. X-ray crystallography (100 K; ammonium phosphate 1.4 M; CHAPSO (w/v) 0.1 %; DTT 1 mM; EGTA 0.5 mM; NaCl 100 mM; NaN3 (w/v) 0.01 %; protein (sample) 10 mg/mL; Tris-HCl 10 mM)
References:
  1. Essen LO, Perisic O, Cheung R, Katan M, Williams RL. "Crystal structure of a mammalian phosphoinositide-specific phospholipase C delta." Nature. 1996; 380(6575): 595-602. PubMed: 8602259
Comments:
 



Region 7
Type:Disordered
Name: 
Location:645 - 650
Length:6
Region sequence:

NKNKNS

Modification type: Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography
References:
  1. Grobler JA, Essen LO, Williams RL, Hurley JH. "C2 domain conformational changes in phospholipase C-delta 1." Nat Struct Biol. 1996; 3(9): 788-95. PubMed: 8784353
Comments:
 



References

  1. Essen LO, Perisic O, Cheung R, Katan M, Williams RL. "Crystal structure of a mammalian phosphoinositide-specific phospholipase C delta." Nature. 1996; 380(6575): 595-602. PubMed: 8602259

  2. Grobler JA, Essen LO, Williams RL, Hurley JH. "C2 domain conformational changes in phospholipase C-delta 1." Nat Struct Biol. 1996; 3(9): 788-95. PubMed: 8784353



Comments


Grobler article is no longer publically available to verify this record.


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