| General information | | DisProt: | DP00061 | | Name: | Replication protein A 70 kDa DNA-binding subunit | | Synonym(s): | RFA1_HUMAN
RP-A p70
Replication factor A protein 1
RF-A protein 1
Single-stranded DNA-binding protein
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | P27694 | | UniGene: | Hs.461925 | | SwissProt: | RFA1_HUMAN | | TrEMBL: | | | NCBI (GI): | 1350579 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 616 | | Percent disordered: | 9% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MVGQLSEGAI AAIMQKGDTN IKPILQVINI RPITTGNSPP RYRLLMSDGL NTLSSFMLAT - 60
QLNPLVEEEQ LSSNCVCQIH RFIVNTLKDG RRVVILMELE VLKSAEAVGV KIGNPVPYNE - 120
GLGQPQVAPP APAASPAASS RPQPQNGSSG MGSTVSKAYG ASKTFGKAAG PSLSHTSGGT - 180
QSKVVPIASL TPYQSKWTIC ARVTNKSQIR TWSNSRGEGK LFSLELVDES GEIRATAFNE - 240
QVDKFFPLIE VNKVYYFSKG TLKIANKQFT AVKNDYEMTF NNETSVMPCE DDHHLPTVQF - 300
DFTGIDDLEN KSKDSLVDII GICKSYEDAT KITVRSNNRE VAKRNIYLMD TSGKVVTATL - 360
WGEDADKFDG SRQPVLAIKG ARVSDFGGRS LSVLSSSTII ANPDIPEAYK LRGWFDAEGQ - 420
ALDGVSISDL KSGGVGGSNT NWKTLYEVKS ENLGQGDKPD YFSSVATVVY LRKENCMYQA - 480
CPTQDCNKKV IDQQNGLYRC EKCDTEFPNF KYRMILSVNI ADFQENQWVT CFQESAEAIL - 540
GQNAAYLGEL KDKNEQAFEE VFQNANFRSF IFRVRVKVET YNDESRIKAT VMDVKPVDYR - 600
EYGRRLVMSI RRSALM
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| Functional narrative |
Replication protein A (RP-A) is a three-subunit single-
stranded DNA-binding protein (composed of RPA70, RPA32, and RPA14 subunits) with important functions in DNA replication, DNA repair and DNA recombination.
The 70 kDa subunit of RP-A (hRPA70) contains an
intrinsically unstructured linker domain (IULD, hRPA70 residues 105-182) that
separates a protein interaction and weak ssDNA binding domain (DBD F, hRPA70
residues 1-104) from two high affinity ssDNA binding domains (DBD A and B, hRPA70 residues 183-422). DBD A, B, and F are structured domains
that form five stranded anti-parallel beta barrels. The IULD functions to enhance
the weak ssDNA binding affinity of DBD F, facilitating the competitive inhibition
of DBD F-protein interactions.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 115 - 168 | | Length: | 54 | | Region sequence: |
PVPYNEGLGQPQVAPPAPAASPAASSRPQPQNGSSGMGSTVSKAYGASKTFGKA | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Flexible linkers/spacers
Protein-DNA binding
Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; pH: 6.5; Varian (spectrometer))
| References:
- Jacobs DM, Lipton AS, Isern NG, Daughdrill GW, Lowry DF, Gomes X, Wold MS. "Human replication protein A: global fold of the N-terminal RPA-70 domain reveals a basic cleft and flexible C-terminal linker." J Biomol NMR. 1999; 14(4): 321-31. PubMed: 10526407
| Comments:
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| References |
- Jacobs DM, Lipton AS, Isern NG, Daughdrill GW, Lowry DF, Gomes X, Wold MS. "Human replication protein A: global fold of the N-terminal RPA-70 domain reveals a basic cleft and flexible C-terminal linker." J Biomol NMR. 1999; 14(4): 321-31. PubMed: 10526407
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| Comments |
Additional UniGene ID: Hs.595562
AV 6-28-2010 PubMed: 10526407
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