| General information | | DisProt: | DP00065 | | Name: | Serine-aspartate repeat-containing protein D | | Synonym(s): | SDRD_STAAE
SdrD protein
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | O86488 | | UniGene: | | | SwissProt: | SDRD_STAAE | | TrEMBL: | | | NCBI (GI): | 75499264 | | Source organism: | Staphylococcus aureus (strain Newman) | | Sequence length: | 1315 | | Percent disordered: | 42% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MLNRENKTAI TRKGMVSNRL NKFSIRKYTV GTASILVGTT LIFGLGNQEA KAAESTNKEL - 60
NEATTSASDN QSSDKVDMQQ LNQEDNTKND NQKEMVSSQG NETTSNGNKL IEKESVQSTT - 120
GNKVEVSTAK SDEQASPKST NEDLNTKQTI SNQEALQPDL QENKSVVNVQ PTNEENKKVD - 180
AKTESTTLNV KSDAIKSNDE TLVDNNSNSN NENNADIILP KSTAPKRLNT RMRIAAVQPS - 240
STEAKNVNDL ITSNTTLTVV DADKNNKIVP AQDYLSLKSQ ITVDDKVKSG DYFTIKYSDT - 300
VQVYGLNPED IKNIGDIKDP NNGETIATAK HDTANNLITY TFTDYVDRFN SVQMGINYSI - 360
YMDADTIPVS KNDVEFNVTI GNTTTKTTAN IQYPDYVVNE KNSIGSAFTE TVSHVGNKEN - 420
PGYYKQTIYV NPSENSLTNA KLKVQAYHSS YPNNIGQINK DVTDIKIYQV PKGYTLNKGY - 480
DVNTKELTDV TNQYLQKITY GDNNSAVIDF GNADSAYVVM VNTKFQYTNS ESPTLVQMAT - 540
LSSTGNKSVS TGNALGFTNN QSGGAGQEVY KIGNYVWEDT NKNGVQELGE KGVGNVTVTV - 600
FDNNTNTKVG EAVTKEDGSY LIPNLPNGDY RVEFSNLPKG YEVTPSKQGN NEELDSNGLS - 660
SVITVNGKDN LSADLGIYKP KYNLGDYVWE DTNKNGIQDQ DEKGISGVTV TLKDENGNVL - 720
KTVTTDADGK YKFTDLDNGN YKVEFTTPEG YTPTTVTSGS DIEKDSNGLT TTGVINGADN - 780
MTLDSGFYKT PKYNLGNYVW EDTNKDGKQD STEKGISGVT VTLKNENGEV LQTTKTDKDG - 840
KYQFTGLENG TYKVEFETPS GYTPTQVGSG TDEGIDSNGT STTGVIKDKD NDTIDSGFYK - 900
PTYNLGDYVW EDTNKNGVQD KDEKGISGVT VTLKDENDKV LKTVTTDENG KYQFTDLNNG - 960
TYKVEFETPS GYTPTSVTSG NDTEKDSNGL TTTGVIKDAD NMTLDSGFYK TPKYSLGDYV - 1020
WYDSNKDGKQ DSTEKGIKDV KVTLLNEKGE VIGTTKTDEN GKYCFDNLDS GKYKVIFEKP - 1080
AGLTQTGTNT TEDDKDADGG EVDVTITDHD DFTLDNGYYE EETSDSDSDS DSDSDSDRDS - 1140
DSDSDSDSDS DSDSDSDSDS DSDSDSDRDS DSDSDSDSDS DSDSDSDSDS DSDSDSDSDS - 1200
DSDSDSDSDS DSDSDSDSDS DSDSDSDSDS DSDSDSDSDS DSDSDSDSDS DSDSDAGKHT - 1260
PVKPMSTTKD HHNKAKALPE TGNENSGSNN ATLFGGLFAA LGSLLLFGRR KKQNK
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| Functional narrative |
Cell surface-associated calcium-binding protein which possibly mediates interactions of S.aureus with components of the extracellular matrix of higher eukaryotes. Has been shown to bind 14-15 calcium ions.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | B1-B5 | | Location: | 569 - 1123 | | Length: | 555 | | Region sequence: |
VYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPNLPNG
DYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKYNLGDYV
WEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGNYKVEFTTP
EGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNYVWEDTNKDGK
QDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEFETPSGYTPTQVG
SGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTNKNGVQDKDEKGISG
VTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYTPTSVTSGNDTEKDSN
GLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLLNEK
GEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEVDVTITD
HDDFTLDNGYYEEET | | Modification type: | Complex
Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | | | Functional subclasses: | Metal binding
| Detection methods:
- Size exclusion/gel filtration chromatography (pH: 7.5; TSK column ; protein (sample) 50 ug; Tris-HCl (buffer) 50 mM; KCl 150 mM; Ca2+ 1 mM; EGTA 1 mM)
- Circular dichroism (CD) spectroscopy, far-UV (pH: 7.5; Jasco J-715 (spectropolarimeter); optical path 1 mm; protein (sample) 0.25 mg/mL; Tris-HCl (buffer) 10 mM)
- Circular dichroism (CD) spectroscopy, near-UV (pH: 7.5; Jasco J-715 (spectropolarimeter); optical path 1 cm; protein (sample) 1.8 mg/mL; Tris-HCl (buffer) 10 mM)
- Fluorescent probes (298 K; CaCl2 5 mM; EGTA 25 um; excitation wavelength 278 nm; guanidine-HCl 4 M; MgCl2 50 mM; Perkin-Elmer LS-5B (spectrofluorimeter); protein (sample) 2 uM; slits 5 nm)
| References:
- Josefsson E, O'Connell D, Foster TJ, Durussel I, Cox JA. "The binding of calcium to the B-repeat segment of SdrD, a cell surface protein of Staphylococcus aureus." J Biol Chem. 1998; 273(47): 31145-52. PubMed: 9813018
| Comments:
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| References |
- Josefsson E, O'Connell D, Foster TJ, Durussel I, Cox JA. "The binding of calcium to the B-repeat segment of SdrD, a cell surface protein of Staphylococcus aureus." J Biol Chem. 1998; 273(47): 31145-52. PubMed: 9813018
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| Comments |
AV (6-29-2010) PubMed: 9813018
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