General information | DisProt: | DP00066_C001 | Name: | Capsid protein | Synonym(s): | POLS_SINDO
Coat protein
C
Cleavage product 1 of Structural polyprotein
| First appeared in release: | Release 1.0 (08/01/2003) | UniProt: | P27285 | UniGene: | | SwissProt: | POLS_SINDO | TrEMBL: | | NCBI (GI): | 130578 | Source organism: | Sindbis virus subtype Ockelbo (strain Edsbyn 82-5)(OCKV)(Ockelbo virus) | Sequence length: | 264 | Percent disordered: | 43% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MNRGFFNMLG RRPFPAPTAM WRPRRRRQAA PMPARNGLAS QIQQLTTAVS ALVIGQATRP - 60 QNPRPRPPPR QKKQAPKQPP KPKKPKPQEK KKKQPAKTKP GKRQRMALKL EADRLFDVKN - 120 EDGDVIGHAL AMEGKVMKPL HVKGTIDHPV LSKLKFTKSS AYDMEFAQLP VNMRSEAFTY - 180 TSEHPEGFYN WHHGAVQYSG GRFTIPRGVG GRGDSGRPIM DNSGRVVAIV LGGADEGTRT - 240 ALSVVTWNSK GKTIKTTPEG TEEW
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Functional narrative |
Capsid protein possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. New virions attach to target cells, and after endocytosis their membrane fuses with the target cell membrane. This leads to the release of the nucleocapsid into the cytoplasm, followed by an uncoating event necessary for the genomic RNA to become accessible. The uncoating might be triggered by the interaction of capsid proteins with ribosomes. Binding of ribosomes would release the genomic RNA since the same region is genomic RNA-binding and ribosome-binding.
Capsid protein is Cleavage product 1 of Structural polyprotein, comprised of aa 1-264 of the polyprotein.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered | Name: | N-terminal | Location: | 1 - 113 | Length: | 113 | Region sequence: |
MNRGFFNMLGRRPFPAPTAMWRPRRRRQAAPMPARNGLASQIQQLTTAVSALVIGQATRP QNPRPRPPPRQKKQAPKQPPKPKKPKPQEKKKKQPAKTKPGKRQRMALKLEAD | Modification type: | Native
| PDB: | | Structural/functional type: | | Functional classes: | | Functional subclasses: | Protein-genomic RNA binding
| Detection methods:
- X-ray crystallography (Siemans (area detector))
| References:
- Choi HK, Tong L, Minor W, Dumas P, Boege U, Rossmann MG, Wengler G. "Structure of Sindbis virus core protein reveals a chymotrypsin-like serine proteinase and the organization of the virion." Nature. 1991; 354(6348): 37-43. PubMed: 1944569
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References |
- Choi HK, Tong L, Minor W, Dumas P, Boege U, Rossmann MG, Wengler G. "Structure of Sindbis virus core protein reveals a chymotrypsin-like serine proteinase and the organization of the virion." Nature. 1991; 354(6348): 37-43. PubMed: 1944569
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Comments |
Previous entry DP00066 has been split into polyprotein DP00066 and cleavage product
DP00066_C001. Disorder is characterized on the cleavage product.
AV (6-29-2010) PubMed: 1944569
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