DP00076: DNA topoisomerase 2FASTA viewXML view

General information
DisProt:DP00076
Name:DNA topoisomerase 2
Synonym(s):TOP2_YEAST
EC 5.99.1.3
DNA topoisomerase II
Top2
Topo II
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P06786
UniGene: 
SwissProt: TOP2_YEAST
TrEMBL:  
NCBI (GI): 1351262
Source organism:Saccharomyces cerevisiae (Baker's yeast)
Sequence length:1428
Percent disordered:25%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MSTEPVSASD KYQKISQLEH ILKRPDTYIG SVETQEQLQW IYDEETDCMI EKNVTIVPGL - 60
FKIFDEILVN AADNKVRDPS MKRIDVNIHA EEHTIEVKND GKGIPIEIHN KENIYIPEMI - 120
FGHLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTEFILET ADLNVGQKYV QKWENNMSIC - 180
HPPKITSYKK GPSYTKVTFK PDLTRFGMKE LDNDILGVMR RRVYDINGSV RDINVYLNGK - 240
SLKIRNFKNY VELYLKSLEK KRQLDNGEDG AAKSDIPTIL YERINNRWEV AFAVSDISFQ - 300
QISFVNSIAT TMGGTHVNYI TDQIVKKISE ILKKKKKKSV KSFQIKNNMF IFINCLIENP - 360
AFTSQTKEQL TTRVKDFGSR CEIPLEYINK IMKTDLATRM FEIADANEEN ALKKSDGTRK - 420
SRITNYPKLE DANKAGTKEG YKCTLVLTEG DSALSLAVAG LAVVGRDYYG CYPLRGKMLN - 480
VREASADQIL KNAEIQAIKK IMGLQHRKKY EDTKSLRYGH LMIMTDQDHD GSHIKGLIIN - 540
FLESSFPGLL DIQGFLLEFI TPIIKVSITK PTKNTIAFYN MPDYEKWREE ESHKFTWKQK - 600
YYKGLGTSLA QEVREYFSNL DRHLKIFHSL QGNDKDYIDL AFSKKKADDR KEWLRQYEPG - 660
TVLDPTLKEI PISDFINKEL ILFSLADNIR SIPNVLDGFK PGQRKVLYGC FKKNLKSELK - 720
VAQLAPYVSE CTAYHHGEQS LAQTIIGLAQ NFVGSNNIYL LLPNGAFGTR ATGGKDAAAA - 780
RYIYTELNKL TRKIFHPADD PLYKYIQEDE KTVEPEWYLP ILPMILVNGA EGIGTGWSTY - 840
IPPFNPLEII KNIRHLMNDE ELEQMHPWFR GWTGTIEEIE PLRYRMYGRI EQIGDNVLEI - 900
TELPARTWTS TIKEYLLLGL SGNDKIKPWI KDMEEQHDDN IKFIITLSPE EMAKTRKIGF - 960
YERFKLISPI SLMNMVAFDP HGKIKKYNSV NEILSEFYYV RLEYYQKRKD HMSERLQWEV - 1020
EKYSFQVKFI KMIIEKELTV TNKPRNAIIQ ELENLGFPRF NKEGKPYYGS PNDEIAEQIN - 1080
DVKGATSDEE DEESSHEDTE NVINGPEELY GTYEYLLGMR IWSLTKERYQ KLLKQKQEKE - 1140
TELENLLKLS AKDIWNTDLK AFEVGYQEFL QRDAEARGGN VPNKGSKTKG KGKRKLVDDE - 1200
DYDPSKKNKK STARKGKKIK LEDKNFERIL LEQKLVTKSK APTKIKKEKT PSVSETKTEE - 1260
EENAPSSTSS SSIFDIKKED KDEGELSKIS NKFKKISTIF DKMGSTSATS KENTPEQDDV - 1320
ATKKNQTTAK KTAVKPKLAK KPVRKQQKVV ELSGESDLEI LDSYTDREDS NKDEDDAIPQ - 1380
RSRRQRSSRA ASVPKKSYVE TLELSDDSFI EDDEEENQGS DVSFNEED

Region 1: 410-420 Region 5: 485-489 Region 7: 527-531 Region 8: 631-652 Region 9: 660-674 Region 2: 634-681 Region 6: 1072-1106 Region 3: 1077-1107 Region 10: 1179-1201 Region 4: 1179-1202 Region 11: 1203-1428

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:410 - 420
Length:11
Region sequence:

NALKKSDGTRK

Modification type: Fragment
PDB: 1BGW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 2
Type:Disordered
Name: 
Location:634 - 681
Length:48
Region sequence:

DKDYIDLAFSKKKADDRKEWLRQYEPGTVLDPTLKEIPISDFINKELI

Modification type: Fragment
PDB: 1BGW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 3
Type:Disordered
Name: 
Location:1077 - 1107
Length:31
Region sequence:

EQINDVKGATSDEEDEESSHEDTENVINGPE

Modification type: Fragment
PDB: 1BGW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787

  2. Dunker AK, Brown CJ, Lawson JD, Iakoucheva LM, Obradović Z. "Intrinsic disorder and protein function." Biochemistry. 2002; 41(21): 6573-82. PubMed: 12022860
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 4
Type:Disordered
Name: 
Location:1179 - 1202
Length:24
Region sequence:

GNVPNKGSKTKGKGKRKLVDDEDY

Modification type: Fragment
PDB: 1BGW:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 5
Type:Disordered
Name: 
Location:485 - 489
Length:5
Region sequence:

SADQI

Modification type: Fragment
Monomeric
Native
PDB: 1BJT:A
Structural/functional type: Function arises from the disordered state
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 6
Type:Disordered
Name: 
Location:1072 - 1106
Length:35
Region sequence:

NDEIAEQINDVKGATSDEEDEESSHEDTENVINGP

Modification type: Fragment
Monomeric
Native
PDB: 1BJT:A
Structural/functional type: Function arises from the disordered state
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 7
Type:Disordered
Name: 
Location:527 - 531
Length:5
Region sequence:

QDHDG

Modification type: Fragment
Monomeric
Native
PDB: 1BJT:A
Structural/functional type: Function arises from the disordered state
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 8
Type:Disordered
Name: 
Location:631 - 652
Length:22
Region sequence:

QGNDKDYIDLAFSKKKADDRKE

Modification type: Fragment
Monomeric
Native
PDB: 1BJT:A
Structural/functional type: Function arises from the disordered state
Functional classes: Entropic chain
Functional subclasses: Flexible linkers/spacers
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 9
Type:Disordered
Name: 
Location:660 - 674
Length:15
Region sequence:

GTVLDPTLKEIPISD

Modification type: Fragment
Monomeric
Native
PDB: 1BJT:A
Structural/functional type: Function arises from the disordered state
Functional classes: Entropic chain
Functional subclasses: Unknown
Flexible linkers/spacers
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 10
Type:Disordered
Name: 
Location:1179 - 1201
Length:23
Region sequence:

GNVPNKGSKTKGKGKRKLVDDED

Modification type: Fragment
Monomeric
Native
PDB: 1BJT:A
Structural/functional type: Function arises from the disordered state
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (277 K; protein 3 uL; reservoir buffer 3 uL)
References:
  1. Berger JM, Gamblin SJ, Harrison SC, Wang JC. "Structure and mechanism of DNA topoisomerase II." Nature. 1996; 379(6562): 225-32. PubMed: 8538787
Comments:
DP00076 - The experimental fragment was composed of residues 410 - 1202.


Region 11
Type:Disordered
Name: 
Location:1203 - 1428
Length:226
Region sequence:

DPSKKNKKSTARKGKKIKLEDKNFERILLEQKLVTKSKAPTKIKKEKTPSVSETKTEEEE
NAPSSTSSSSIFDIKKEDKDEGELSKISNKFKKISTIFDKMGSTSATSKENTPEQDDVAT
KKNQTTAKKTAVKPKLAKKPVRKQQKVVELSGESDLEILDSYTDREDSNKDEDDAIPQRS
RRQRSSRAASVPKKSYVETLELSDDSFIEDDEEENQGSDVSFNEED

Modification type: Monomeric
Native
PDB:  
Structural/functional type: Function arises from the disordered state
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
 
References:
  1. Dunker AK, Brown CJ, Lawson JD, Iakoucheva LM, Obradović Z. "Intrinsic disorder and protein function." Biochemistry. 2002; 41(21): 6573-82. PubMed: 12022860

  2. Shaiu WL, Hu T, Hsieh TS. "The hydrophilic, protease-sensitive terminal domains of eucaryotic DNA topoisomerases have essential intracellular functions." Pac Symp Biocomput. 1999: 578-589. PubMed: 10380229
Comments:
The C-terminal domain is enriched in charged amino acids and hypersensitive to protease digestion, both indications that the region is disordered.


Comments


PMID 8538787, erratum in: Nature 1996 Mar 14;380(6570):179.


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