| General information | | DisProt: | DP00077 | | Name: | Regulatory protein ADR1 | | Synonym(s): | ADR1_YEAST
Transcription factor ADR1
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | P07248 | | UniGene: | | | SwissProt: | ADR1_YEAST | | TrEMBL: | | | NCBI (GI): | 56757368 | | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | | Sequence length: | 1323 | | Percent disordered: | 3% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MANVEKPNDC SGFPVVDLNS CFSNGFNNEK QEIEMETDDS PILLMSSSAS RENSNTFSVI - 60
QRTPDGKIIT TNNNMNSKIN KQLDKLPENL RLNGRTPSGK LRSFVCEVCT RAFARQEHLK - 120
RHYRSHTNEK PYPCGLCNRC FTRRDLLIRH AQKIHSGNLG ETISHTKKVS RTITKARKNS - 180
ASSVKFQTPT YGTPDNGNFL NRTTANTRRK ASPEANVKRK YLKKLTRRAS FSAQSASSYA - 240
LPDQSSLEQH PKDRVKFSTP ELVPLDLKNP ELDSSFDLNM NLDLNLNLDS NFNIALNRSD - 300
SSGSTMNLDY KLPESANNYT YSSGSPTRAY VGANTNSKNA SFNDADLLSS SYWIKAYNDH - 360
LFSVSESDET SPMNSELNDT KLIVPDFKST IHHLKDSRSS SWTVAIDNNS NNNKVSDNQP - 420
DFVDFQELLD NDTLGNDLLE TTAVLKEFEL LHDDSVSATA TSNEIDLSHL NLSNSPISPH - 480
KLIYKNKEGT NDDMLISFGL DHPSNREDDL DKLCNMTRDV QAIFSQYLKG EESKRSLEDF - 540
LSTSNRKEKP DSGNYTFYGL DCLTLSKISR ALPASTVNNN QPSHSIESKL FNEPMRNMCI - 600
KVLRYYEKFS HDSSESVMDS NPNLLSKELL MPAVSELNEY LDLFKNNFLP HFPIIHPSLL - 660
DLDLDSLQRY TNEDGYDDAE NAQLFDRLSQ GTDKEYDYEH YQILSISKIV CLPLFMATFG - 720
SLHKFGYKSQ TIELYEMSRR ILHSFLETKR RCRSTTVNDS YQNIWLMQSL ILSFMFALVA - 780
DYLEKIDSSL MKRQLSALCS TIRSNCLPTI SANSEKSINN NNEPLTFGSP LQYIIFESKI - 840
RCTLMAYDFC QFLKCFFHIK FDLSIKEKDV ETIYIPDNES KWASESIICN GHVVQKQNFY - 900
DFRNFYYSFT YGHLHSIPEF LGSSMIYYEY DLRKGTKSHV FLDRIDTKRL ERSLDTSSYG - 960
NDNMAATNKN IAILIDDTII LKNNLMSMRF IKQIDRSFTE KVRKGQIAKI YDSFLNSVRL - 1020
NFLKNYSVEV LCEFLVALNF SIRNISSLYV EEESDCSQRM NSPELPRIHL NNQALSVFNL - 1080
QGYYYCFILI IKFLLDFEAT PNFKLLRIFI ELRSLANSIL LPTLSRLYPQ EFSGFPDVVF - 1140
TQQFINKDNG MLVPGLSANE HHNGASAAVK TKLAKKINVE GLAMFINEIL VNSFNDTSFL - 1200
NMEDPIRNEF SFDNGHRAVT DLPRSAHFLS DTGLEGINFS GLNDSHQTVS TLNLLRYGEN - 1260
HSSKHKNGGK GQGFAEKYQL SLKYVTIAKL FFTNVKENYI HCHMLDKMAS DFHTLENHLK - 1320
GNS
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| Functional narrative |
The high-affinity binding region of the protein ADR-1 binds to DNA, and with the aid of zinc fingers forms a molecular complex. ADR-1, located in the nucleus, is a transcription factor and is essential in the activation of alcohol dehydrogenase 2 (ADH2) in Saccharomyces cerevisiae (Baker’s Yeast). During glucose repression, Ser-230 is phosphorylated, which prevents the transcription of ADH2. It binds to the activation sequence UAS1 in the promoter region of ADH2.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | | | Location: | 75 - 104 | | Length: | 30 | | Region sequence: |
MNSKINKQLDKLPENLRLNGRTPSGKLRSF | | Modification type: | Engineered
| | PDB: | 1ADR:A, 2ADR:A | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (310 K; pH: 6.8; )
- Nuclear magnetic resonance (NMR) (288 K; pH: 6.1; )
- Nuclear magnetic resonance (NMR) (290 K; pH: 6.3; )
| References:
- Hartshorne TA, Blumberg H, Young ET. "Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA." Nature. 1986; 320(6059): 283-7. PubMed: 3515197
- Hyre DE, Klevit RE. "A disorder-to-order transition coupled to DNA binding in the essential zinc-finger DNA-binding domain of yeast ADR1." Journal of Molecular Biology. 1998; 279(4): 929-943. PubMed: 9642072
| Comments:
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| Region 2 | | Type: | Disordered - Extended | | Name: | | | Location: | 155 - 161 | | Length: | 7 | | Region sequence: |
HSGNLGE | | Modification type: | Engineered
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (310 K; pH: 6.8; )
- Nuclear magnetic resonance (NMR) (288 K; pH: 6.1; )
- Nuclear magnetic resonance (NMR) (290 K; pH: 6.3; )
| References:
- Hartshorne TA, Blumberg H, Young ET. "Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA." Nature. 1986; 320(6059): 283-7. PubMed: 3515197
- Hyre DE, Klevit RE. "A disorder-to-order transition coupled to DNA binding in the essential zinc-finger DNA-binding domain of yeast ADR1." Journal of Molecular Biology. 1998; 279(4): 929-943. PubMed: 9642072
| Comments:
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| References |
- Hartshorne TA, Blumberg H, Young ET. "Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA." Nature. 1986; 320(6059): 283-7. PubMed: 3515197
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