DP00083: General control protein GCN4FASTA viewXML view

General information
DisProt:DP00083
Name:General control protein GCN4
Synonym(s):GCN4_YEAST
Amino acid biosynthesis regulatory protein
Transcription factor GCN4
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P03069
UniGene: 
SwissProt: GCN4_YEAST
TrEMBL:  
NCBI (GI): 121066
Source organism:Saccharomyces cerevisiae (Baker's yeast)
Sequence length:281
Percent disordered:20%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD - 60
TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW - 120
TSLFDNDIPV TTDDVSLADK AIESTEEVSL VPSNLEVSTT SFLPTPVLED AKLTQTRKVK - 180
KPNSVVKKSH HVGKDDESRL DHLGVVAYNR KQRSIPLSPI VPESSDPAAL KRARNTEAAR - 240
RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R

Region 1: 225-281

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:DNA binding domain
Location:225 - 281
Length:57
Region sequence:

SDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER

Modification type: Complex
Native
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes:  
Functional subclasses: Protein-DNA binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; KCl 200 mM; potassium phosphate 50 mM)

  2. Size exclusion/gel filtration chromatography
References:
  1. Weiss MA, Ellenberger T, Wobbe CR, Lee JP, Harrison SC, Struhl K. "Folding transition in the DNA-binding domain of GCN4 on specific binding to DNA." Nature. 1990; 347(6293): 575-8. PubMed: 2145515
Comments:
 


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