MRLRKYNKSLGWLSLFAGTVLLSGCN

1. X-ray crystallography (PEG 1500 (approximate (w/v)) 9 %; sodium chloride 100 mM; magnesium chloride 100 mM; ethanol ((v/v)) 5 %)

1. Abramson J, Riistama S, Larsson G, Jasaitis A, Svensson-Ek M, Laakkonen L, Puustinen A, Iwata S, Wikstrom M. "The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site." Nat Struct Biol. 2000; 7(10): 910-7. PubMed: 11017202
   
   

This region is located in the periplasmic space.

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Abramson et al. could not assign a sequence for residues 1-36 or 113-124. However the PDB file locates electron density for residues 27-283.

HGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH

1. X-ray crystallography (ethanol ((v/v)) 5 %; magnesium chloride 100 mM; PEG 1500 (approximate (w/v)) 9 %; sodium chloride 100 mM)

1. Abramson J, Riistama S, Larsson G, Jasaitis A, Svensson-Ek M, Laakkonen L, Puustinen A, Iwata S, Wikstrom M. "The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site." Nat Struct Biol. 2000; 7(10): 910-7. PubMed: 11017202
   
   

This region is located in the periplasmic space.

MRLRKYNKSLGWLS

1. X-ray crystallography (277 K; ammonium sulfate (precipitant) 35 %; citrate/bis-Tris-propane ((pH 9.0-9.5)) 100 mM; dimethylsulphoxide ((to obtain sharp edges) v/v) 2 %; DTT (buffer) 1 mM; EDTA (buffer) 1 mM; NaN3 (buffer) 1 mM)

1. van der Oost J, Lappalainen P, Musacchio A, Warne A, Lemieux L, Rumbley J, Gennis RB, Aasa R, Pascher T, Malmstrom BG, et al. "Restoration of a lost metal-binding site: construction of two different copper sites into a subunit of the E. coli cytochrome o quinol oxidase complex." Embo J. 1992; 11(9): 3209-17. PubMed: 1324168
   
   
2. van der Oost J, Musacchio A, Pauptit RA, Ceska TA, Wierenga RK, Saraste M. "Crystallization and preliminary X-ray analysis of the periplasmic fragment of CyoA-a subunit of the Escherichia coli cytochrome o complex." J Mol Biol. 1993; 229(3): 794-6. PubMed: 8433374
   
   
3. Wilmanns M, Lappalainen P, Kelly M, Sauer-Eriksson E, Saraste M. "Crystal structure of the membrane-exposed domain from a respiratory quinol oxidase complex with an engineered dinuclear copper center." Proc Natl Acad Sci U S A. 1995; 92(26): 11955-9. PubMed: 8618822
   
   

This region is located in the periplasmic space.

AHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH

1. X-ray crystallography (277 K; ammonium sulfphate (precipitant) 35 %; citrate/bis-Tris-propane ((pH 9.0-9.5)) 100 mM; dimethylsuphoxide ((to obtain sharp edges) v/v) 2 %; DTT (buffer) 1 mM; EDTA (buffer) 1 mM; NaN3 (buffer) 1 mM)

1. van der Oost J, Lappalainen P, Musacchio A, Warne A, Lemieux L, Rumbley J, Gennis RB, Aasa R, Pascher T, Malmstrom BG, et al. "Restoration of a lost metal-binding site: construction of two different copper sites into a subunit of the E. coli cytochrome o quinol oxidase complex." Embo J. 1992; 11(9): 3209-17. PubMed: 1324168
   
   
2. van der Oost J, Musacchio A, Pauptit RA, Ceska TA, Wierenga RK, Saraste M. "Crystallization and preliminary X-ray analysis of the periplasmic fragment of CyoA-a subunit of the Escherichia coli cytochrome o complex." J Mol Biol. 1993; 229(3): 794-6. PubMed: 8433374
   
   
3. Wilmanns M, Lappalainen P, Kelly M, Sauer-Eriksson E, Saraste M. "Crystal structure of the membrane-exposed domain from a respiratory quinol oxidase complex with an engineered dinuclear copper center." Proc Natl Acad Sci U S A. 1995; 92(26): 11955-9. PubMed: 8618822
   
   

This region is located in the periplasmic space.

1. Chepuri V, Lemieux L, Au DC, Gennis RB. "The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases." J Biol Chem. 1990; 265(19): 11185-92. PubMed: 2162835
   
   

A periplasmic fragment of subunit II is located under the PDB identification of 1CYW. It is residues 125-283 of subunit II as described by Abramson et. al.