Annotations for this protein have been verified by the authors of the corresponding papers



DP00101_C006: p6-gagFASTA viewXML view

General information
DisProt:DP00101_C006
Name:p6-gag
Synonym(s):GAG_HV1N5
cleavage product 6 of Gag polyprotein
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P12493
UniGene: 
SwissProt: GAG_HV1N5
TrEMBL:  
NCBI (GI): 120839
Source organism:Human immunodeficiency virus type 1 (isolate NY5 group M subtype B)(HIV-1)
Sequence length:52
Percent disordered:69%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
LQSRPEPTAP PEESFRFGEE TTTPSQKQEP IDKELYPLAS LRSLFGSDPS SQ



Functional narrative    

p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1. p6-gag is cleavage product 6 of Gag polyprotein, comprised of aa 449-500 of the polyprotein.

Region 1: 1-13 Region 2: 19-32 Region 3: 44-52

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:1 - 13
Length:13
Region sequence:

LQSRPEPTAPPEE

Modification type: Engineered
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (pH: 7.2; Phosphate buffer; protein 0.2 mg/ml)

  2. Nuclear magnetic resonance (NMR) (308 K; pH: 3; H2O (super distilled); TFE-d2)

References:
  1. Fossen T, Wray V, Bruns K, Rachmat J, Henklein P, Tessmer U, Maczurek A, Klinger P, Schubert U. "Solution structure of the human immunodeficiency virus type 1 p6 protein." J Biol Chem. 2005. PubMed: 16234236

Comments:
 



Region 2
Type:Disordered
Name:flexible hinge region
Location:19 - 32
Length:14
Region sequence:

EETTTPSQKQEPID

Modification type: Engineered
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (pH: 7.2; Phosphate buffer; protein 0.2 mg/ml)

  2. Nuclear magnetic resonance (NMR) (308 K; pH: 3; H2O (super distilled); TFE-d2)

References:
  1. Fossen T, Wray V, Bruns K, Rachmat J, Henklein P, Tessmer U, Maczurek A, Klinger P, Schubert U. "Solution structure of the human immunodeficiency virus type 1 p6 protein." J Biol Chem. 2005. PubMed: 16234236

Comments:
 



Region 3
Type:Disordered
Name: 
Location:44 - 52
Length:9
Region sequence:

LFGSDPSSQ

Modification type: Engineered
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (pH: 7.2; Phosphate buffer; protein 0.2 mg/ml)

  2. Nuclear magnetic resonance (NMR) (308 K; pH: 3; H2O (super distilled); TFE-d2)

References:
  1. Fossen T, Wray V, Bruns K, Rachmat J, Henklein P, Tessmer U, Maczurek A, Klinger P, Schubert U. "Solution structure of the human immunodeficiency virus type 1 p6 protein." J Biol Chem. 2005. PubMed: 16234236

Comments:
 



Comments


The sequence given is the sequence of the Pr55 Gag polyprotein from HIV Type 1. p6 protein is the C-terminal part of Pr55. In its mature state p6 protein is free from Pr55. This entry is for the mature free state of p6. Secondary structure, elucidated by CD and NMR spectroscopy, strongly depends on the structure-stabilizing effect of TFE.



Previous entry DP00101 has been split into polyprotein (DP00101) and cleavage product DP00101_C006. Disorder is characterized on the cleavage product.


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